Prepared September 30, 2016 for Kim Malloy on Behalf of Buckley Report ID: 00BAN 0287MARS0000V

Prepared September 30, 2016 for Kim Malloy on Behalf of Buckley Report ID: 00BAN 0287MARS0000V

Prepared September 30, 2016 for Kim Malloy on behalf of Buckley Report ID: 00BAN_0287MARS0000V Dr. Mia L Alphonso Banfield Pet Hospital #0287 3221 PEACHTREE RD, STE 250, ATLANTA, GA 30305 Fax: (404) 237-7789 Canine Genetic Analysis™ – What to Expect We’re excited to provide you this report about our findings of Buckley’s genetic heritage. You might confirm your suspicions or be surprised. Either way, you’ll get a glimpse into your dog’s heritage and find information that will help you care for Buckley in insightful ways. Dr. Alphonso also received a detailed report including disease screening results that may impact Buckley’s health, as well as an individualized Optimum Wellness Plan® map. This is designed to help provide the best quality of life working with Buckley through Banfield Pet Hospital’s unique preventive care model. This report includes: Buckley’s Geneology Breed Characteristics MDR1 Screening Results Genetic Heritage Optimum Wellness Plan: What’s Next? Certification of Genetic Ancestry Genealogy Buckley’s Ancestry Buckley is a Chihuahua, Pug, Collie, Parson Russell Terrier Cross! The Canine Genetic Analysis™ performs approximately seven million calculations using 11 models to predict the most likely combination of pure breeds and mixed breeds in the last three ancestral generations. This chart presents the best statistical result of the analysis: Collie* Parson Russell Chihuahua Chihuahua Chihuahua Chihuahua Pug Pug (US Show) Terrier* Chihuahua Chihuahua Pug / Collie Pug / Parson Russell Cross Terrier Cross Buckley Chihuahua Pug / Collie / Parson Russell Terrier Cross Learn more about the Breeds detected in Buckley on the following pages... *Breed detected, however at a lower confidence. Such results are not included in accuracy calculations. 3 Breed Characteristics Chihuahua Height: 6 - 9 in Weight (Show): 2 - 7.5 lb Weight (Pet): 2 - 9 lb Chihuahua Ears: Muzzle: Tail: The Chihuahua is one of the most ancient of dog breeds, with its origins dating back to the Mayan civilization. The breed is believed to have descended from the ancient Techichi, a dog that played an important role in both the Toltec and Aztec cultures. Some believe that the Chihuahua is a result of the crossing of the Techichi and the Chinese Crested, which came to South America from Alaska. Due to the destruction of the ancient civilizations, the breed faced extinction. However, remnants of the breed survived in Mexico and the Chihuahua was introduced to the United States in 1898. The Chihuahua was recognized as an official breed in 1904 by the American Kennel Club and by 1915, thirty Chihuahuas were registered in the States with that number jumping to over 25,000 by the early seventies. The Chihuahua has a vast amount of variation partly due to the fact that any color or combination is permitted in the breed standard. The coat comes in either long or short hair, with traits including dark masks, merle, and white markings. The colors range from blacks to various silver and fawn shades. Do you recognize any of these Chihuahua traits in Buckley? Small size makes it easy for them to live in Alert, active, and often playful dogs. smaller places such as apartments. Responds well to reward-based training using May be suspicious of strangers or bark at other treats or favorite toys. dogs when intimidated by their size. 4 Breed Characteristics Pug Height: 12 - 16 in Weight (Show): 14 - 18 lb Weight (Pet): Pug 12 - 24 lb Ears: Muzzle: Tail: Pugs are an ancient breed, dating back to the Shang Dynasty of China in the years 1766 through 1122 BC. They were kept as pets by Tibetan monks before being exported to Japan, and then later to Europe. In the late sixteenth and early seventeenth centuries, sailors of the Dutch East India Trading Company brought Pugs back with them to Holland. Shortly thereafter, the Pug became the official dog of the Dutch Royal family. Pugs soon became popular throughout Europe, particularly in England, Spain, Germany and France. Pugs accompanied William and Mary to the British throne in 1688 and Napoleon is believed to have used a Pug to deliver messages to his wife when she was imprisoned. Pugs became very popular in nineteenth century England as they were a favorite of Queen Victoria, who bred them whilst keeping them as pets. Pugs of that time were of the fawn or apricot color, but darker shades were introduced when an English aristocrat brought some back from China. Standards for the breed were established in the 1880’s and the Pug was recognized by the American Kennel Club in 1885. The Pug generally comes in fawn or black, though the fawn varies from a pale cream to a more vibrant apricot color. There is also a silver variant. The pug generally has a contrasting black mask which sometimes includes black or shaded markings on the ears or forehead. Do you recognize any of these Pug traits in Buckley? Pugs are usually good with children and often Active, playful, clever, and rambunctious dogs. are friendly dogs. Need regular activity, however require less Stubborn tendencies may be lessened by using exercise than other breeds. Can participate reward-based training involving small treats and in dog sports such as agility and rally or favorite toys. competitive obedience. 5 Breed Characteristics Collie Height: 20 - 24 in Weight (Show): 45 - 75 lb Weight (Pet): Collie 44 - 79 lb Ears: Muzzle: Tail: The Collie originated in Scotland, where they were bred for hundreds of years to work as shepherds and guard dogs. Today the Collie is one of the most popular breeds in the world. Over the years, the Collie has developed an amazing level of intelligence and the breed has been used for herding, protection, water rescue and as a guide for the blind. Queen Victoria, J.P. Morgan, Jim Reeves, Cecil Hepworth, Brooke Langton, Mario Lemieux, David Duchovny, President Coolidge and President Hoover have all owned Collies at some point during history. The Collie’s reputation for bravery, loyalty and intelligence was further enforced after the release of the film, “Lassie Come Home” in 1943 and popularity soared through the ‘40’s and ‘50’s when the TV Lassie series idealized the Collie as a family dog. The health of the breed suffered somewhat as indiscriminate breeding took place to capitalize upon the breed’s popularity, but today Collies remain consistently popular with pet owners. The breed received American Kennel Club recognition in 1885. The DNA detected from this breed is most similar to DNA sampled from American Kennel Club registered dogs. The four main AKC coat descriptions are "Sable and White" (Sable is a variety of tan shades), "Tri-Color" (Mainly black with white and tan markings), "Blue Merle" (A mottled coat of various shades) and "White" (One of the other types with more white in the coat). The coat may be either short (Smooth) or long (Rough). Blazes of white across the muzzle and chest are common in this breed. Do you recognize any of these Collie traits in Buckley? Collies are eager to learn especially with Intelligent, obedient, active, and watchful dogs. reward-based training, Collies seem to enjoy dog sports such as agility, Although they are often good with children and flyball, Frisbee, tracking, rally and competitive are usually friendly, some Collies can be wary or obedience. reserved with strangers. 6 Breed Characteristics Parson Russell Terrier Height: 11 - 15 in Weight (Show): 13 - 17 lb Weight (Pet): Terrier 11 - 23 lb Ears: Muzzle: Tail: Parson Russell “Parson Russell Terrier” is the name of the show variant of the commonly known “Jack Russell" or "Russell Terrier” breed, although all types are relatively difficult to distinguish genetically. The Parson Russell is descended from early white-bodied working terriers. The breed was developed by Reverend John Russell during the 1800’s as a fox hunter. Their small bodies allowed them to drive the fox from his lair, giving the hunters easy access. The name Parson Russell was chosen by the American Kennel Club because the name “Jack Russell Terrier” had been trademarked by a group opposed to allowing the working terrier breed into the Kennel Club. The theory was that Parson Russells are show dogs, whereas Jack Russells are working dogs. The breed was first introduced to the United States in the 1930’s. Parson Russell Terriers were first recognized by the UK Kennel Club in 1990 and the American Kennel Club in 1997. The Parson Russell Terrier may have a broken (wirehaired) or smooth coat, and is predominantly white with black, tan, cream, or black and tan markings. Grizzle (some black hairs among the coat) is also possible. Do you recognize any of these Parson Russell Terrier traits in Buckley? Parson Russell Terriers enjoy tennis ball Very intelligent, obedient, active, agile, highly retrieving, agility, flyball, and Frisbee, and have energetic, and watchful dogs. the keen sense of smell necessary to participate in earthdog trials. Eager to learn, especially with reward-based May be difficult to disengage from an activity or training. behavior, bark, or chase wildlife or other dogs. 7 How Traits Are Inherited How Genetics Influence Breed Appearance and Behavior This report includes common behavioral and physical traits associated with each of the breeds we’ve detected in your dog’s DNA. But remember, the link between genes and their expression in specific dogs is complex. It’s likely that your dog exhibits characteristics of each breed in different ways – some more subtle than others. An Example of Breed Expression in an Individual Dog We found three primary breeds in our example dog, Frankie. While overall, Frankie is one-of-a-kind, certain aspects of Frankie’s behavior and appearance indicate the influence of each of these breeds.

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