HLUTIRAMOUNTUS 20170295783A1 ( 19) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2017/ 0295783 A1 LI et al. ( 43) Pub . Date : Oct . 19 , 2017 (54 ) COMPOSITIONS AND METHODS TO Publication Classification SELECTIVELY CONTROL SPECIES (51 ) Int. Cl. ( 71 ) Applicant: STEPHEN F . AUSTIN STATE AOIN 35 /02 ( 2006 .01 ) UNIVERSITY , Nacogdoches, TX ( US ) AOIN 65 / 18 (2009 . 01 ) AOIN 65 /04 ( 2009 .01 ) ( 72 ) Inventors: Shiyou LI, Nacogdoches , TX (US ) ; A01N 35 / 06 ( 2006 .01 ) Ping WANG , Nacogdoches, TX (US ) ; A01N 65 /03 ( 2009. 01 ) Wei YUAN , Nacogdoches, TX (US ) ; A01N 43 / 20 ( 2006 .01 ) Zushang SU , Nacogdoches, TX (US ) ; AOIN 65 /40 (2009 . 01 ) Steven H . BULLARD , Nacogdoches , A01N 65 / 06 (2009 . 01) TX (US ) ( 52 ) U . S . CI. CPC .. .. .. .. A01N 35 / 02 ( 2013 .01 ) ; A01N 65 /40 ( 21) Appl . No. : 15 /523 , 576 (2013 .01 ) ; A01N 65 / 18 ( 2013 . 01 ) ; AOIN 65 /06 (2013 .01 ) ; AOIN 35 / 06 ( 2013 . 01 ) ; ( 22 ) PCT Filed : Nov. 3 , 2015 AOIN 65 / 03 ( 2013 .01 ) ; AOIN 43 / 20 ( 86 ) PCT No. : PCT/ US15 /58817 (2013 .01 ) ; AOIN 65 / 04 ( 2013 .01 ) $ 371 (c ) ( 1 ) , (57 ) ABSTRACT ( 2 ) Date : May 1, 2017 Methods and compositions for controlling an invasive or Related U . S . Application Data unwanted species by application of a composition comprised (60 ) Provisional application No . 62 /074 ,426 , filed on Nov. of an endocide derived from the same species or a closely 3 , 2014 . related species. DADID , Sig = 280 , 8 Ret = 360 , 80 (WATER SAMPLE 06- 0601. 0 ) PAUL Day 1 100 507TITI:TIT ITT DADID . SG - 250 , 5 R & t= 360 . 60 ( WATER SAMPLE 006- ODL . DE - - -- - -- - 25 -- - - mint HAU ; Day 2 100TTTTTTT: * ** DADI D , Sig = 260 ,8 Rel: 360, 80 (WATER SAMPLE10110 01. D ITAU Day 3 TTTTTIT: . : - ) 20 DADID , Sig = 280 , 8 14360 .BO (WATER SAMOLEV013 - 0 - 01. 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D ) 25 MAU Day 8 777-7 - - - - Mlima 0 * * * * * * * * * * * * * * * * * * * - * * * - - - - - * * * * * * * * 10 15 DADIÇ , Siga254 , 8 Rel360 .80 LOATING HEART :303 - 0104 . D ) mmmmmmmmmm. 25mmmmmmmmm ???? ????? ??n 6 Day 9 & U ?????????????? - - - 20 mint FIG . 2A Patent Application Publication Oct. 19 , 2017 Sheet 3 of 10 US 2017 /0295783 A1 W ruiserrurursuri HPLCPeakArea(254nm) " - ?????????? Š rrrrrrrrrr O Ferrrrrrrrrrrrr! . 1 2 3 4 5 6 7 8 9 Days FIG . 2B Patent Application Publication Oct. 19 , 2017 Sheet 4 of 10 US 2017 /0295783 A1 DADI D , Sig = 280 , 8 Rot - 360 ,80 (WATER SAMPLE _006 - 0601. D ) - - - - - - - - - - - - - - MAU Day 1 88kivéverilu TTTT T 10 15 20 . .. - DADI D , Sig = 280 , 8 Rel360 ,80 (WATER SAMPLE \008 -0101 . 03 25 .. .. .. MAU uatui. Day 2 -FFF4F PIE S L - - - - - - - - - - - - - - - - LAc - - - - - - - - - - * DADI D, Sig =20 , 8 HEX 310 BO WATER SLAPLEXIT- 01010 ) . .. .. 20. .. .. 25 .. .. 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MAU 100 calien,14.- Day 7 V .. .. .. - - - - - so - - - - - - - - - - - - - - - - - - - - - - - - - 7 - - - - - - - - - - - - - * * - - - - YYYYYY DAD # D , Sig = 280 . 8 Ref - 360 .80 ( FLOATING HEART /001 - 0301. D MAU ltexia.-L 100 Day 8 - - -- ALLERIET 1 set . wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww p owym wwwwwwwwwww w wwwwwwwwwwwww DADID , Sig = 280 , 8 R - 360 , 80 ( FLOATING10 HEART1O03 - 0101. 0 ) * . .. 20 25 .. .. .. .. mu ; Day 9 100 viewfinal. w - - - - VW ww. * D nnaconcepts - - - - - - - - - - os . .. 25 min FIG . 2C Patent Application Publication Oct. 19 , 2017 Sheet 5 of 10 US 2017 /0295783 A1 12000 10000 Tillillllllllllll ???????????????????????????????????????????????? XXXXXXXXXXXXXXXY HPLCPeakArea(280nm) ? ???bao VU 1 2 3 4 5 6 7 8 9 Days FIG . 2D Patent Application Publication Oct. 19 , 2017 Sheet 6 of 10 US 2017 /0295783 A1 DAD1 C -, - - Sig- - - - - =- - -254 - - - - - ,- - 8 - Ref- - -= 360- - - ,80 ( FLOATING HEART1004 - 0101. D ) - - - - - - - - - - Day 1 - - - ????????????????? . S Y - - - WwwwwwwwV a DAD1 C , Sig = 254 .8 Ref = 360, 80 ( FLOATING HEART\ 003 - 0101 . 0 ) 15 _ _ _ 20 _ _ _ 25 __ _ _ mini MAU Day 2 - - - -- -- - .. m - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - T T - - - - - - - - DAD 1 C , Sig = 254 .8 Ret= 360 ,80 (FLOATING HEART\ 018 -0101 . D ) 15 25 . min MAU Day 3 m - - . - -- - - www?LLLLLLLLLLLS - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - T - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 10 71 DAD1 C , Sig = 254 , 8 Ref = 360 .80 (FLOATING HEART1019 - 0201. D ) MAU Day 4 Muh INa mmad ???????????????????? 7 - - - - - - - - 20 25 _ _ _ _ _ DAD ? C , Sig - 254 , 8 Ref 360 ,80 ( FLOATING HEART1016 - 0401. D ) -- 2 Day 5 - - - V . - -- - - -- - .- - - . — .. - - -- - - - w ??????????????????????? ** * ** -- - - -- - - - 10 15 25 DAD1 C , Sig = 254 . 8 Ref = 360 ,80 (FLOATING HEART1015 - 0301. D ) MAU Day 6 V we - - - LLLLLLLLLLLLLL - - - - 20 25 DAD1 C , Sig = 254 , 8 Ref= 360 ,80 (FLOATING HEART \014 -0101 . D ) min MAU Day 7 wi -- - . - . - . ?????????? - - - - - - - - - - - - - - - - - - - - - - 10 15 min DAD1 C , Sig = 254 . 8 Ref = 360 , 80 ( FLOATING HEART 013 -0101 , D ) 20 25 Day 8 - - - - C - - - - - - - - - - - - - - - - - - - - - - - - ?????????? - - - - 15 min DAD1 C , Sig = 254 . 8 Ref = 360 ,80 ( FLOATING HEART1012 - 0201. D ) MAU : Day 9 V enice TTTTTTT min . FIG . 3A Patent Application Publication Oct. 19 , 2017 Sheet 7 of 10 US 2017 /0295783 A1 9000 8000 turn HPLCPeakArea(254nm) 7000 6000 separerepreneur +EFFIZIFFFFFFFFFFFFFFF:FFFFFF 5000 4000 REFRA 3000 2000 ???????????????????. 1000 ???? 0 ... - - * * * * * F4127777777777777777777777777777777277778Fri12EF1FFFFFFFF17FF112FF12/Frie:27174877887177777777777777777788Frrr 1 2 3 4 5 6 7 8 9 Days FERREFERREFERREFERREFERREIRA FIG . 3B Patent Application Publication Oct. 19 , 2017 Sheet 8 of 10 US 2017 /0295783 A1 DAD1 D , Sig = 280 , 8 Ret = 360 , 80 ( FLOATING HEART \004 -0101 . D ) MAU : Day 1 3 ma - - - - - - - - - - - - - o .. - - - - - - - - TT wwwwwwwwwwwwwwww DAD1 D . Sig = 280 . 8 Ref = 360, 80 (FLOATING HEART\ 003 -0101 . D ) MAU 1 Day 2 50 TIN 0 - 2 med hud 10 2020 25 DAD1 D , Sig = 280 , 8 Ref = 360 , 80 (FLOATING HEART \018 -0101 . D ) MAU Day 3 50 - 1 meer . wo - . - - - - - - - A - - - - - TTTT T TTTTT min DAD1 D , Sig = 280 ,8 Ref = 360 , 89 (FLOATING HEART \019