Supplementary Table S1. List of all the proteins identified by iTRAQ analysis. OVSA OVISE OVMA RMG- JHOS4 OVCA OVSA SKOV Seque OVISE OVMA RMG- JHOS4 OVCA OVSA HO/H SKOV # OVISE /HOSE OVMA NA/HO RMG- I/HOS JHOS4 /HOSE OVCA R3/HO HO/H SKOV 3/HOS Accession nce MW calc. Subcellular /HOSE NA/HO I/HOS /HOSE R3/HO HO/H OSE2 3/HOS Pept # AAs Score Protein name /HOSE 2C NA/HO SE2C I/HOS E2C /HOSE 2C R3/HO SE2C OSE2 3/HOS E2C number covera [kDa] pI localization 2C SE2C E2C 2C SE2C OSE2 C E2C ides 2C Variabi SE2C Variabi E2C Variabi 2C Variabi SE2C Variabi C E2C Variabi ge(%) Count Count Count Count Count C Variabi Count lity [%] lity [%] lity [%] lity [%] lity [%] Count lity [%] lity [%] Extended synaptotagmin-2 OS=Homo sapiens GN=ESYT2 PE=1 SV=1 - Plasma A0FGR8 13.9 10 921 102.29 9.26 421.7 1.043 12 19.8 0.758 9 15.3 0.969 11 43.5 1.080 11 27.3 0.800 10 34.7 3.281 12 74.1 1.430 9 38.6 [ESYT2_HUMAN] membrane A0MZ66 3.65 2 631 71.60 5.33 49.5 Shootin-1 OS=Homo sapiens GN=KIAA1598 PE=1 SV=4 - [SHOT1_HUMAN] Other 2.515 3 33.6 1.495 3 13.1 1.985 2 56.9 1.684 3 5.4 1.561 3 31.4 1.881 3 22.2 2.783 3 32.1 Acetolactate synthase-like protein OS=Homo sapiens GN=ILVBL PE=1 SV=2 - A1L0T0 10.13 5 632 67.82 8.15 231.2 Other 0.820 4 20.3 0.675 4 37.0 0.833 4 37.6 0.880 4 44.3 0.789 4 37.8 3.677 4 78.5 0.929 4 13.0 [ILVBL_HUMAN] Uncharacterized protein KIAA0564 OS=Homo sapiens GN=KIAA0564 PE=1 A3KMH1 1.15 2 1905 214.69 7.40 30.9 Secreted 0.934 1 0.661 1 1.650 2 28.7 1.928 2 18.0 0.949 2 38.8 1.748 2 10.7 2.450 1 SV=2 - [K0564_HUMAN] WD repeat-containing protein 91 OS=Homo sapiens GN=WDR91 PE=1 SV=2 - A4D1P6 1.61 1 747 83.29 6.58 24.7 Other 1.837 1 [WDR91_HUMAN] Putative Rab-43-like protein ENSP00000330714 OS=Homo sapiens PE=5 SV=3 A6NDJ8 12.15 1 181 20.20 6.54 156.1 Nucleus 0.849 1 3.087 1 0.157 1 0.330 1 0.880 1 1.538 1 0.307 1 - [RB43L_HUMAN] Structural maintenance of chromosomes flexible hinge domain-containing protein A6NHR9 1.2 2 2005 226.23 7.30 53.1 Other 0.964 3 39.5 0.777 2 86.9 0.701 2 42.7 0.367 3 8.0 0.523 3 8.5 0.854 3 14.6 1.209 2 58.4 1 OS=Homo sapiens GN=SMCHD1 PE=1 SV=2 - [SMHD1_HUMAN] Ras-related protein Rap-1b-like protein OS=Homo sapiens PE=2 SV=1 - Plasma A6NIZ1 10.87 2 184 20.91 5.48 99.1 0.879 2 20.3 0.757 1 0.625 2 17.8 1.050 2 48.6 1.058 2 73.6 5.280 2 61.2 1.755 2 91.1 [RP1BL_HUMAN] membrane Putative Golgi pH regulator C OS=Homo sapiens GN=GPR89C PE=5 SV=2 - A6NKF9 3.75 1 320 36.65 9.32 82.2 Other 1.186 2 47.0 1.270 2 17.5 1.179 2 41.2 0.993 2 55.1 0.781 2 22.2 1.859 2 132.7 1.795 1 [GPHRC_HUMAN] Putative tropomyosin alpha-3 chain-like protein OS=Homo sapiens PE=5 SV=2 - A6NL28 9.87 1 223 26.25 4.51 61.1 Cytoplasm 0.536 1 0.906 1 0.792 1 0.313 1 0.876 1 1.324 1 [TPM3L_HUMAN] Protein FAM171A2 OS=Homo sapiens GN=FAM171A2 PE=1 SV=1 - A8MVW0 1.94 1 826 87.38 8.05 63.6 Other 0.570 1 0.200 1 0.333 1 0.324 1 0.490 1 1.642 1 0.343 1 [F1712_HUMAN] Uroplakin-3b-like protein OS=Homo sapiens GN=UPK3BL PE=2 SV=1 - Plasma B0FP48 8.37 2 263 28.37 8.21 152.2 1.319 4 44.8 0.494 2 8.7 0.525 3 5.6 3.651 4 105.9 6.590 4 72.9 1.625 4 24.0 0.697 2 123.5 [UPK3L_HUMAN] membrane Alkyldihydroxyacetonephosphate synthase, peroxisomal OS=Homo sapiens Peroxisom O00116 3.8 2 658 72.87 7.34 49.1 0.800 2 4.1 1.047 2 52.8 1.288 2 87.3 0.639 2 142.2 1.358 2 112.6 1.594 2 69.7 1.359 1 GN=AGPS PE=1 SV=1 - [ADAS_HUMAN] e Kinesin-like protein KIF2A OS=Homo sapiens GN=KIF2A PE=1 SV=3 - O00139 1.56 1 706 79.90 6.68 51.4 Nucleus 1.194 2 17.7 1.049 2 17.8 1.254 2 8.8 0.886 2 10.3 1.230 1 1.111 2 0.6 [KIF2A_HUMAN] ATP-dependent RNA helicase DDX39A OS=Homo sapiens GN=DDX39A PE=1 O00148 13.35 2 427 49.10 5.68 327.9 Nucleus 1.494 3 31.9 1.067 3 44.0 1.342 3 61.3 1.206 3 23.0 1.413 3 1.1 2.217 3 11.1 1.175 3 12.9 SV=2 - [DX39A_HUMAN] Plasma O00159 7.71 8 1063 121.61 9.41 179.5 Myosin-Ic OS=Homo sapiens GN=MYO1C PE=1 SV=4 - [MYO1C_HUMAN] 0.581 10 40.3 0.370 10 53.8 0.438 10 32.8 0.334 9 84.9 0.618 10 56.3 1.729 10 45.3 0.636 10 113.7 membrane Plasma O00168 13.04 1 92 10.43 9.14 195.8 Phospholemman OS=Homo sapiens GN=FXYD1 PE=1 SV=2 - [PLM_HUMAN] 0.392 1 0.532 1 2.333 1 1.538 1 1.142 2 39.1 12.872 2 94.8 7.189 1 membrane Armadillo repeat protein deleted in velo-cardio-facial syndrome OS=Homo Plasma O00192 3.85 2 962 104.58 6.81 44.1 0.697 1 0.876 1 1.594 1 sapiens GN=ARVCF PE=1 SV=1 - [ARVC_HUMAN] membrane O00214 6.94 2 317 35.79 8.25 22.3 Galectin-8 OS=Homo sapiens GN=LGALS8 PE=1 SV=4 - [LEG8_HUMAN] Cytoplasm 1.235 2 17.6 0.257 2 64.4 0.748 2 22.0 0.529 2 210.2 0.609 2 142.9 0.821 2 963.2 Tumor necrosis factor receptor superfamily member 10A OS=Homo sapiens Plasma O00220 5.34 2 468 50.06 7.03 37.1 1.452 2 20.4 1.469 2 53.9 1.339 2 62.8 1.249 2 174.4 1.142 2 359.0 0.908 2 37.4 1.348 2 99.4 GN=TNFRSF10A PE=1 SV=3 - [TR10A_HUMAN] membrane 26S proteasome non-ATPase regulatory subunit 11 OS=Homo sapiens O00231 10.9 4 422 47.43 6.48 53.0 Cytoplasm 1.238 4 38.2 0.865 4 52.4 1.090 3 7.6 0.703 3 6.2 1.068 4 32.2 1.661 4 13.8 1.409 1 GN=PSMD11 PE=1 SV=3 - [PSD11_HUMAN] 26S proteasome non-ATPase regulatory subunit 12 OS=Homo sapiens O00232 4.61 2 456 52.87 7.65 27.7 Cytoplasm 1.239 2 8.4 1.215 2 116.2 1.917 2 81.3 0.907 2 67.1 0.956 2 186.8 1.803 2 42.8 1.733 2 73.5 GN=PSMD12 PE=1 SV=3 - [PSD12_HUMAN] Signal-regulatory protein beta-1 OS=Homo sapiens GN=SIRPB1 PE=1 SV=5 - Plasma O00241 4.27 2 398 43.18 6.52 55.7 0.658 2 7.8 0.438 2 16.6 0.848 2 13.6 0.403 2 49.2 0.518 2 2.8 0.442 2 97.4 1.160 2 21.6 [SIRB1_HUMAN] membrane Copper transport protein ATOX1 OS=Homo sapiens GN=ATOX1 PE=1 SV=1 - O00244 11.76 1 68 7.40 7.24 21.0 Cytoplasm 0.502 1 0.414 1 0.647 1 0.374 1 0.296 1 1.094 1 [ATOX1_HUMAN] Membrane-associated progesterone receptor component 1 OS=Homo sapiens Plasma O00264 17.44 2 195 21.66 4.70 160.4 1.005 3 60.9 0.866 2 126.8 0.743 2 16.5 0.490 3 14.7 1.616 2 126.2 4.441 3 72.2 0.663 1 GN=PGRMC1 PE=1 SV=3 - [PGRC1_HUMAN] membrane Transcription elongation factor SPT5 OS=Homo sapiens GN=SUPT5H PE=1 O00267 4.23 5 1087 120.92 5.06 105.8 Nucleus 1.071 6 22.8 0.772 5 50.8 0.946 6 40.2 0.815 6 38.8 2.000 6 59.9 2.074 6 49.3 1.094 4 70.6 SV=1 - [SPT5H_HUMAN] DNA fragmentation factor subunit alpha OS=Homo sapiens GN=DFFA PE=1 O00273 5.14 1 331 36.50 4.79 36.9 Cytoplasm 1.403 2 36.7 1.472 1 1.327 1 1.631 2 79.3 1.586 2 51.8 SV=1 - [DFFA_HUMAN] Chloride intracellular channel protein 1 OS=Homo sapiens GN=CLIC1 PE=1 Plasma O00299 3.73 1 241 26.91 5.17 23.6 0.833 1 0.484 1 0.843 1 0.672 1 0.743 1 0.793 1 0.616 1 SV=4 - [CLIC1_HUMAN] membrane Eukaryotic translation initiation factor 3 subunit F OS=Homo sapiens GN=EIF3F O00303 7.28 2 357 37.54 5.45 97.2 Cytoplasm 1.693 3 28.5 0.883 3 3.6 1.219 3 10.0 0.736 3 5.0 1.055 3 2.4 0.679 3 34.2 1.473 2 40.5 PE=1 SV=1 - [EIF3F_HUMAN] NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Homo sapiens GN=WWP2 O00308 1.15 1 870 98.85 7.12 39.6 Cytoplasm 0.170 1 0.252 1 0.412 1 0.287 1 0.314 1 0.367 1 0.622 1 PE=1 SV=2 - [WWP2_HUMAN] Pyruvate dehydrogenase protein X component, mitochondrial OS=Homo sapiens Mitochondr O00330 4.59 2 501 54.09 8.66 141.3 1.446 2 55.9 1.065 2 41.5 1.392 2 14.8 1.998 2 54.9 1.210 2 10.4 3.350 2 41.1 1.236 2 2.1 GN=PDHX PE=1 SV=3 - [ODPX_HUMAN] ion O00410 6.29 5 1097 123.55 4.94 121.8 Importin-5 OS=Homo sapiens GN=IPO5 PE=1 SV=4 - [IPO5_HUMAN] Cytoplasm 1.640 6 22.7 0.578 6 56.6 1.071 7 23.5 0.610 5 48.5 0.994 6 74.4 1.732 7 58.3 1.081 5 44.3 DNA-directed RNA polymerase, mitochondrial OS=Homo sapiens GN=POLRMT Mitochondr O00411 1.46 2 1230 138.53 8.98 117.8 0.938 1 0.726 1 0.938 1 1.226 1 1.515 1 3.081 1 1.465 1 PE=1 SV=2 - [RPOM_HUMAN] ion Insulin-like growth factor 2 mRNA-binding protein 3 OS=Homo sapiens O00425 5.53 2 579 63.67 8.87 110.6 Nucleus 1.588 2 82.5 0.654 1 1.111 2 33.5 1.049 2 45.5 1.001 2 23.8 1.206 2 10.5 0.503 1 GN=IGF2BP3 PE=1 SV=2 - [IF2B3_HUMAN] Dynamin-1-like protein OS=Homo sapiens GN=DNM1L PE=1 SV=2 - Plasma O00429 4.89 3 736 81.83 6.81 62.1 1.317 3 3.0 0.910 2 16.1 1.218 2 15.8 1.024 2 5.0 1.410 2 15.0 1.260 3 31.9 2.115 3 15.2 [DNM1L_HUMAN] membrane Plasma O00468 9.19 14 2045 214.71 6.40 477.0 Agrin OS=Homo sapiens GN=AGRN PE=1 SV=4 - [AGRIN_HUMAN] 1.343 16 41.1 1.503 15 50.8 0.859 14 49.6 0.777 14 45.1 1.376 15 61.3 2.676 16 70.1 1.459 11 48.7 membrane Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 OS=Homo sapiens Plasma O00469 1.63 1 737 84.63 6.71 20.0 0.397 1 0.597 1 0.873 1 0.885 1 0.596 1 0.740 1 1.877 1 GN=PLOD2 PE=1 SV=2 - [PLOD2_HUMAN] membrane Butyrophilin subfamily 3 member A1 OS=Homo sapiens GN=BTN3A1 PE=1 Plasma O00481 2.53 1 513 57.64 8.03 47.7 0.728 1 1.258 1 0.635 1 0.465 1 0.624 1 0.580 1 0.858 1 SV=3 - [BT3A1_HUMAN] membrane NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 OS=Homo Mitochondr O00483 37.04 3 81 9.36 9.38 55.7 1.146 4 10.7 0.918 4 99.7 1.739 4 6.5 0.711 4 60.5 0.809 5 32.6 4.211 5 41.1 1.393 4 58.3 sapiens GN=NDUFA4 PE=1 SV=1 - [NDUA4_HUMAN] ion Plasma O00515 1.74 1 517 57.10 9.67 38.7 Ladinin-1 OS=Homo sapiens GN=LAD1 PE=1 SV=2 - [LAD1_HUMAN] 1.006 1 1.236 1 1.361 1 1.457 1 2.050 1 1.108 1 membrane Pescadillo homolog OS=Homo sapiens GN=PES1 PE=1 SV=1 - O00541 2.89 2 588 67.96 7.33 60.5 Nucleus 0.655 2 9.9 0.655 2 45.9 0.744 2 65.0 0.666 2 42.6 0.705 2 132.3 1.271 2 19.8 [PESC_HUMAN] Nucleolar protein 56 OS=Homo sapiens GN=NOP56 PE=1 SV=4 - O00567 14.98 7 594 66.01 9.19 179.9 Nucleus 1.045 9 35.3 0.648 9 57.6 1.100 8 28.8 1.112 9 27.3 1.064 9 14.4 2.927 10 75.3 1.113 8 42.0 [NOP56_HUMAN] ATP-dependent RNA helicase DDX3X OS=Homo sapiens GN=DDX3X PE=1 O00571 13.6 7 662 73.20 7.18 285.6 Nucleus 0.598 12 25.5 0.346 11 43.3 0.580 11 42.6 0.584 12 14.9 0.436 12 39.3