Ascentis® ES Cyano HPLC Columns Key Benefits Cyano columns have been used for the analysis of Tricyclic Antidepressants for some time. A comparison of the most popular competitor column and the Ascentis L Extra stable for low pH mobile phases due to sterically ES Cyano phase is shown below. Better resolution is seen using the Ascentis ES protected phase Cyano than the competitor column for three critical peak pairs. In addition, note the selectivity change of the trimipramine and doxepin (peaks 1 and 2) between these L Improved retention compared two phases under these conditions to other cyano phases L Endcapped for better peak shape at neutral pH Tricyclic Antidepressants on Ascentis ES Cyano and a Competitor Cyano Phase column: as indicated; 15 cm x 4.6 mm, 5 μm particle mobile phase A: 10 mM potassium phosphate, dibasic (pH 7.0 with phosphoric acid) mobile phase B: acetonitrile 1. Trimipramine mobile phase C: methanol 2. Doxepin mixing proportions: A:B:C, 25:60:15 3. Amitriptyline flow rate: 2.0 mL/min 4. Imipramine temp.: 25 °C 5. Nordoxepin det.: 215 nm 2 6. Nortriptyline injection: 5 μL Ascentis ES Cyano 7. Desipramine sample: 100 μg /mL in 70:30, 8. Protriptyline water: methanol 3 5 1 4 6 7 8 0 2 4 6 8 10 12 14 2 Min G004971 3 Competitor Cyano 1 5 4 6 7 8 02468101214 Min G004972 Trimipramine Doxepin Amitriptylin Imipramine G004973 G004974 G004975 G004976 Desipramine Nordoxepin Nortriptyline Protriptyline G004977 G004979 G004980 G004978 Extended Stability of Ascentis ES Cyano Compared to Traditional Cyano Columns using Acidic Mobile Phases 2.5 2.0 Ascentis ES Cyano 1.5 k’ Traditional Cyano 1.0 0.5 0 0102030405060 Void Volumes (x 1,000) G004981 Ascentis ES Cyano HPLC Columns Length (cm) ID (mm) Cat. No. Length (cm) ID (mm) Cat. No. 3 μm 5 μm 5 2.1 577308-U 5 2.1 577300-U 10 2.1 577309-U 10 2.1 577301-U 15 2.1 577310-U 15 2.1 577303-U 5 4.6 577311-U 5 4.6 577304-U 10 4.6 577312-U 10 4.6 577305-U 15 4.6 577306-U 25 4.6 577307-U TRADEMARKS: Ascentis – Sigma-Aldrich Biotechnology LP ( & ")( Order/Customer Service (800) 325-3010 ,Fax (800) 325-5052 World Headquarters ') ( ) ( ) #&&*( && Technical Service (800) 325-5832 ,sigma-aldrich.com/techservice ($) % $ ' &Life Science, Development/Custom Manufacturing Inquiries (800) 244-1173 !!! sigma-aldrich.com High Technology& Service Safety-related Information sigma-aldrich.com/safetycenter 1 #!& %*#" ( %%*#!",+*+*. ' *,*&*$+%('!#'!,(#!& %*#" ('#,+2%#,#!& %*#" MPH #(,"'(%(!0#!&*')*(-,+*+(%,"*(-!"#!& %*#"'#!& %*#"'/**',+,",#,+)*(-,+(' (*&,(,"#' (*&,#('(',#'#',"#+'(,"*#!& %*#" )-%#,#('+-*"+*&-+,,*&#',"+-#,#%#,0( ,")*(-,+ (*,"#*)*,#-%*-+ #,#('%,*&+'('#,#('+&0))%0%++*.*++#( ,"#'.(#(*)$#'!+%#) T410070A.