Course ID: MICR R100L Curriculum Committee Approval Date: 01/14/2015 Catalog Start Date: Fall 2015 COURSE OUTLINE OXNARD COLLEGE I. Course Identification and Justification: A. Proposed course id: MICR R100L Banner title: Principles of Microbiology Lab Full title: Principles of Microbiology Laboratory Previous course id: MICR R100L Banner title: Principles of Microbiology Lab Full title: Principles of Microbiology Laboratory B. Reason(s) course is offered: This course is required for professional degree and certificate programs such as nursing and dental hygiene; it is an elective for the biology degree. This course is transferable to the CSU and UC and provides credit in the following areas (A1 OC GE, B3 CSU GE; 5C IGETC). C. Reason(s) for current outline revision: In this revision, prerequisites are being modified so that they are listed for each prior course in complete (Math, Biology, English, Physiology and Anatomy). Students are entering the OC Micro course without all the prerequisites because they took these courses at other schools that have different sequential prerequisite series. The textbook was also updated. D. C-ID: 1. C-ID Descriptor: 2. C-ID Status: E. Co-listed as: Current: None Previous: II. Catalog Information: A. Units: Current: 2.00 Previous: 2.00 B. Course Hours: 1. In-Class Contact Hours: Lecture: 0 Activity: 0 Lab: 105 2. Total In-Class Contact Hours: 105 3. Total Outside-of-Class Hours: 0 4. Total Student Learning Hours: 105 C. Prerequisites, Corequisites, Advisories, and Limitations on Enrollment: 1. Prerequisites Current: MICR R100: Principles of Microbiology or Previous: MICR R100: Principles of Microbiology 2. Corequisites Current: MICR R100: Principles of Microbiology Previous: 3. Advisories: Current: Previous: 4. Limitations on Enrollment: Current: Previous: D. Catalog description: Current: This is a laboratory course designed for biological science majors and students interested in the health science professions. The exercises are intended to give the students experience in the manipulation of microorganisms and exposure to current microbial techniques. Topics covered will include microscopy methods, prokaryotic and eukaryotic cell structure, microbial metabolism, genetics, recombinant DNA, and biotechnology. Previous, if different: E. Fees: Current: $ None Previous, if different: $ F. Field trips: Current: Will be required: [ ] May be required: [X] Will not be required: [ ] Previous, if different: Will be required: [ ] May be required: [ ] Will not be required: [ ] G. Repeatability: Current: A - Not designed as repeatable Previous: A - Not designed as repeatable H. Credit basis: Current: Letter Graded Only [X] Pass/No Pass [ ] Student Option [ ] Previous, if different: Letter Graded Only [ ] Pass/No Pass [ ] Student Option [X] I. Credit by exam: Current: Petitions may be granted: [ ] Petitions will not be granted: [X] Previous, if different: Petitions may be granted: [ ] Petitions will not be granted: [ ] III. Course Objectives: Upon successful completion of this course, the student should be able to: A. Demonstrate the principles of asepsis B. Operate a light microscope C. Perform standard bacteriological staining procedures D. Cultivate, transfer and maintain pure microbial cultures E. Explain an understanding of the principles of fermentation and applied microbiology F. Perform modern recombinant DNA technology methods G. Analyze the role of microbes in the biosphere H. Identify the role of normal body flora I. State an understanding of colonization and infectious disease J. Determine the identity of a simple unknown bacterium K. Discuss the role of microbiology in medicine, food production, biotechnology and agriculture IV. Student Learning Outcomes: A. The student will have the ability to proceed stepwise biochemical laboratory tests in solving the identity of an unknown bacterium. B. The student will be capable of producing a laboratory notebook, indexed and paginated with the current semester's schedule. V. Course Content: Topics to be covered include, but are not limited to: A. Microscopy and microbial diversity 1. Care and use of the bright-field light microscope 2. Care and use of the fluorescence microscope 3. Care and use of the phase contrast microscope 4. Observation of bacteria, microscopic eukaryotes, and blood cells (slides). 5. Observation of microscopic organisms from environmental sources B. Basic Staining Techniques 1. Gram 2. Acid Fast 3. PHB 4. Endospore 5. Flagellar 6. Fluorescent C. Microbial Growth 1. Growth 2. Measurement of Growth 3. Phases of Growth in Closed Systems 4. Maintenance of pure culture D. Nutrition and Cultivation 1. Nutritional Requirements 2. Culture Conditions 3. Media 4. Culture Characteristics E. Methods of Identification 1. Entertube II or API method 2. PCR-Sequencing 3. Standard keys utilizing biochemical tests F. Antibiotic Sensitivity Testing Methods 1. Kirby-Bauer 2. E-Test G. Quality Control Methods 1. Plate Counts 2. Coliform Testing H. Investigation of Geochemical Microbiology 1. Biogas and Alcohol production 2. Winogradsky column I. Biotechnologies 1. Transformation 2. RT-PCR 3. DNA Extraction 4. DNA Sequencing (outsourced) 5. Phage plaque assay and purification J. Immunological Assays 1. Agglutination 2. Elisa 3. Western Blot 4. Immunoprecipitation K. Eukaryotic Cell Culture 1. Cell Culture Methods L. Applied Microbiology 1. Plant Virus: TMV 2. Animal Virus: Bacculovirus 3. Microbes used in food production M. Medical and Dental Microbiology 1. Specialized media for skin, throat, and urogenital cultures 2. Specialized media for oral cultures 3. Sampling methods for determination of cancer (oral, GI, skin) N. Aseptic technique 1. Determination of disinfectant and sanitizer effectiveness 2. Operation of the laminar flow biosafety cabinet 3. Appropriate use of gloves, gowns and goggles VI. Lab Content: A. Microscopy and microbial diversity 1. Care and use of the bright-field light microscope 2. Care and use of the fluorescence microscope 3. Care and use of the phase contrast microscope 4. Observation of bacteria, microscopic eukaryotes, and blood cells (slides). 5. Observation of microscopic organisms from environmental sources B. Basic Staining Techniques 1. Gram 2. Acid Fast 3. PHB 4. Endospore 5. Flagellar 6. Fluorescent C. Microbial Growth 1. Growth 2. Measurement of Growth 3. Phases of Growth in Closed Systems 4. Maintenance of pure culture D. Nutrition and Cultivation 1. Nutritional Requirements 2. Culture Conditions 3. Media 4. Culture Characteristics E. Methods of Identification 1. Entertube II or API method 2. PCR-Sequencing 3. Standard keys utilizing biochemical tests F. Antibiotic Sensitivity Testing Methods 1. Kirby-Bauer 2. E-Test G. Quality Control Methods 1. Plate Counts 2. Coliform Testing H. Investigation of Geochemical Microbiology 1. Biogas and Alcohol production 2. Winogradsky column I. Biotechnologies 1. Transformation 2. RT-PCR 3. DNA Extraction 4. DNA Sequencing (outsourced) 5. Phage plaque assay and purification J. Immunological Assays 1. Agglutination 2. Elisa 3. Western Blot 4. Immunoprecipitation K. Eukaryotic Cell Culture 1. Cell Culture Methods L. Applied Microbiology 1. Plant Virus: TMV 2. Animal Virus: Baculovirus 3. Microbes used in food production M. Medical and Dental Microbiology 1. Specialized media for skin, throat, and urogenital cultures 2. Specialized media for oral cultures 3. Sampling methods for determination of cancer (oral, GI, skin) N. Aseptic technique 1. Determination of disinfectant and sanitizer effectiveness 2. Operation of the laminar flow biosafety cabinet 3. Appropriate use of gloves, gowns and goggles VII. Methods of Instruction: Methods may include, but are not limited to: A. Short lectures using computer data and the blackboard/whiteboard to explain the procedures of microscopy, staining, dilutions, biochemical analysis, genetics, virology, immunology and recombinant DNA technologies. B. Laboratory exercises completed individually for solving the unknown bacterial sample or in groups of two or more for performing other exercises. C. The use of computer slides, overheads, video and other media to illustrate recombinant DNA technologies and other conceptually difficult topics D. Structured one-on-one interactions with students to support, monitor, and evaluate progress in completing a laboratory notebook, computer data presentation and laboratory methods related to sterile techniques, staining and solving the bacterial unknown. E. Use of a student generated laboratory book and/or report assignments and presentations to help create a study guide for efficient examination preparation. F. A pre-examination practice lab practical to familiarize student with the exam mode. G. Daily oral quizzing, group discussion and student driven current events sections of the class. H. A detailed review of the material as a take home assignment before students have been given the opportunity to take a test with similar material. Other methods of instruction: I. Short lectures using computer data and the blackboard/whiteboard to explain the procedures of microscopy, staining, dilutions, biochemical analysis, genetics, virology, immunology and recombinant DNA technologies. J. Laboratory exercises completed individually for solving the unknown bacterial sample or in groups of two or more for performing other exercises. K. The use of computer slides, overheads, video and other media to illustrate recombinant DNA technologies and other conceptually difficult topics L. Structured one-on-one interactions