RESEARCH ARTICLE A kinase-dependent feedforward loop affects CREBB stability and long term memory formation Pei-Tseng Lee1, Guang Lin1, Wen-Wen Lin1, Fengqiu Diao2, Benjamin H White2, Hugo J Bellen1,3,4,5,6* 1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States; 2Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, United States; 3Howard Hughes Medical Institute, Baylor College of Medicine, Houston, United States; 4Program in Developmental Biology, Baylor College of Medicine, Houston, United States; 5Department of Neuroscience, Baylor College of Medicine, Houston, United States; 6Jan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, United States Abstract In Drosophila, long-term memory (LTM) requires the cAMP-dependent transcription factor CREBB, expressed in the mushroom bodies (MB) and phosphorylated by PKA. To identify other kinases required for memory formation, we integrated Trojan exons encoding T2A-GAL4 into genes encoding putative kinases and selected for genes expressed in MB. These lines were screened for learning/memory deficits using UAS-RNAi knockdown based on an olfactory aversive conditioning assay. We identified a novel, conserved kinase, Meng-Po (MP, CG11221, SBK1 in human), the loss of which severely affects 3 hr memory and 24 hr LTM, but not learning. Remarkably, memory is lost upon removal of the MP protein in adult MB but restored upon its *For correspondence: reintroduction. Overexpression of MP in MB significantly increases LTM in wild-type flies showing [email protected] that MP is a limiting factor for LTM. We show that PKA phosphorylates MP and that both proteins synergize in a feedforward loop to control CREBB levels and LTM. key words: Drosophila, Competing interest: See page 14 Mushroom bodies, SBK1, deGradFP, T2A-GAL4, MiMIC DOI: https://doi.org/10.7554/eLife.33007.001 Funding: See page 14 Received: 21 October 2017 Accepted: 19 February 2018 Published: 23 February 2018 Introduction Forward genetic screens coupled with classical conditioning paradigms (Tully and Quinn, 1985) Reviewing editor: K have been successful in identifying many molecular determinants of learning and memory in Dro- VijayRaghavan, National Centre sophila (Guven-Ozkan and Davis, 2014). Among the genes identified are several components of for Biological Sciences, Tata the cAMP signaling pathway, including dunce (a cAMP specific phosphdiesterase), rutabaga (a Institute of Fundamental Research, India cAMP specific adenylyl cyclase), Dc0 (Protein Kinase A, PKA) and CREBB (a transcription factor) (see Figure 1—figure supplement 1 for these and others) (McGuire et al., 2005). In adult flies, these This is an open-access article, genes act in cells of the mushroom bodies (MB), higher brain centers responsible for associating con- free of all copyright, and may be ditioned stimuli (CS) and unconditioned stimuli (US), and for storing these associations (Dubnau and freely reproduced, distributed, Tully, 1998). Associations are stored by the MB Kenyon cells (KC), which are activated by CS—such transmitted, modified, built upon, or otherwise used by as odors—via cholinergic transmission from olfactory projection neurons and US—such as electric 2+ anyone for any lawful purpose. shock—via dopamine signaling (McGuire et al., 2005). Acetylcholine receptors permit Ca entry 2+ The work is made available under into KCs, while dopamine receptors activate a Ca /Calmodulin-responsive adenylyl cyclase (ruta- the Creative Commons CC0 baga), which acts as a coincidence detector (Busto et al., 2010). cAMP produced by Rutabaga acti- public domain dedication. vates PKA and triggers a downstream MAP kinase cascade, which leads to the phosphorylation and Lee et al. eLife 2018;7:e33007. DOI: https://doi.org/10.7554/eLife.33007 1 of 16 Research article Neuroscience activation of the transcription factor CREBB (Impey et al., 1999). CREBB binds to cAMP response elements (CRE), activating the transcription of genes required for long term memory formation (DeZazzo and Tully, 1995). CREBB is phosphorylated by several kinases, including PKA and CamKII, two kinases known to be involved in memory formation (Horiuchi et al., 2004; Mayr and Montminy, 2001)(Figure 1—figure supplement 1). Here we describe the identification of a novel gene Meng-Po (MP), that is required for memory formation but not for learning. The MP protein regulates the stability of CREBB together with PKA. In the absence of MP the protein stability of CREBB is affected, and removing a single copy of MP and PKA leads to a dramatic loss of CREBB and memory formation. In addition, overex- pression of MP strongly promotes memory formation, indicating that MP is not only required but that it also plays an instructive role in memory formation. Results Genes encoding protein kinases expressed in MB in adult brain and behavioral consequences of RNA interference To identify novel protein kinases involved in learning/memory, we developed a strategy that allows us to determine which kinases are expressed in MBs. We selected 27 putative kinase-encoding genes for which fly lines were available that contained intronic insertions of the Minos Mediated Inte- gration Casette (MiMIC) (Nagarkar-Jaiswal et al., 2015; Venken et al., 2011). These MiMICs con- tain a swappable cassette, allowing integration of any DNA using recombinase-mediated cassette exchange (RMCE). We replaced these cassettes with a Trojan exon encoding SA-T2A-GAL4 to per- mit the detection of cells expressing the kinase-encoding genes using UAS-mCD8::GFP (Diao et al., 2015). Of the 27 putative protein kinase genes screened, we found 12 that are expressed in MBs (Figure 1a; Figure 1—figure supplement 2). To determine if these 12 kinases expressed in MB play a role in learning and memory we knocked down their expression using UAS-RNAi expressed under the control of the MB-selective OK107-GAL4 driver (Figure 1—figure supplement 3a) and tested the performance of learning and 3 hr memory in knockdown flies upon an olfactory aversive condi- tioning assay (Figure 1; Figure 1—figure supplement 3b). In Drosophila, Pavlovian olfactory aver- sive learning requires coincidence detection of a conditioned stimulus (CS), an odor, and an unconditioned stimulus (US), an electric shock. Through a single training session using a T-maze assay in which flies are exposed to 12 CS-US pairings in one min, flies can associate CS with US and learn to avoid the odor paired with electric shock (Tully and Quinn, 1985). After training, flies can learn (tested immediately) and form an intermediate-term/3 hr memory (tested after 3 hr) (Margulies et al., 2005). We identified two putative kinases, CG11221 and wallenda (wnd), which when knocked down, cause significant reductions in performance index for 3 hr memory, but not for learning (Figure 1b–c). Overexpression of wnd has been documented to enhance memory in Dro- sophila (Huang et al., 2012), but CG11221 has not been previously characterized in flies. We there- fore tested the effects of knocking down CG11221 expression using a second RNAi expressed specifically in MB neurons to confirm the memory loss phenotype (Figure 1—figure supplement 3b). To provide additional evidence that CG11221 is not required for learning, we used a short CS/ US association protocol (30’ CS + US instead of 60’) to train flies. Knockdown of MP did not affect learning, further indicating that MP is not required for learning (Figure 1—figure supplement 3b). CG11221 is an evolutionarily conserved serine/threonine protein kinase (human SBK1: 37% iden- tity and 75% similarity, Figure 1—figure supplement 4). SBK1 is expressed in the hippocampus, cor- tex, and cerebellum of adult rodents and loss of SBK1 in mice causes partial embryonic lethality (Nara et al., 2001; Skarnes et al., 2011). The CG11221MI03008-T2A-GAL4; UAS-mCherry flies exhibit broad expression of the gene in third instar larvae and adult flies (Figure 1—figure supplement 5). As shown in Figure 1a, the gene is also expressed in the adult brain and is prominent in the MB. In light of its importance in memory, we renamed CG11221, Meng-Po (MP), for the Lady of Forgetful- ness, a character in Chinese mythology who ensures that people are ready for reincarnation by pro- viding the ‘Tea of Forgetfulness’ so they lose the memory associated with their former life. Lee et al. eLife 2018;7:e33007. DOI: https://doi.org/10.7554/eLife.33007 2 of 16 Research article Neuroscience Figure 1. Genes encoding kinases expressed in MB of adult brains and behavioral consequences of RNA interference. (a) Expression patterns of genes encoding protein kinases in adult brains. The 12 protein kinases shown here are expressed in MB. (b–c) Screening results of behavioral assays by RNAi knockdown. The MB-GAL4 driver, OK107 was used to drive the UAS-RNAi. Flies were raised at 18˚C until eclosion, transferred to 25˚C for 3 days and behavioral assays were performed to test (b) learning and (c) 3 hr memory. The mean ±SEM is plotted for each genotype; n = 8 for each group. *p<0.05, **p<0.01. DOI: https://doi.org/10.7554/eLife.33007.002 The following figure supplements are available for figure 1: Figure supplement 1. Overview of the proteins required for olfactory aversive learning/memory formation in Drosophila. DOI: https://doi.org/10.7554/eLife.33007.003 Figure supplement 2. The T2A-GAL4 expression patterns of protein kinases that are not expressed in MB. DOI: https://doi.org/10.7554/eLife.33007.004