For Research Use Only PRKAR2A Polyclonal antibody Catalog Number:10142-2-AP Featured Product 2 Publications www.ptglab.com Basic Information Catalog Number: GenBank Accession Number: Purification Method: 10142-2-AP BC002763 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150UL , Concentration: 400 μg/ml by 5576 WB 1:500-1:2000 Nanodrop and 313 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:2000 method using BSA as the standard; protein kinase, cAMP-dependent, for WB Source: regulatory, type II, alpha IF 1:10-1:100 Rabbit Calculated MW: Isotype: 43 kDa IgG Observed MW: Immunogen Catalog Number: 46-52 kDa AG0203 Applications Tested Applications: Positive Controls: IF, IP, WB,ELISA WB : K-562 cells, HeLa cells, human testis tissue, MCF-7 Cited Applications: cells, mouse testis tissue, PC-3 cells IHC, WB IP : HeLa cells, K-562 cells Species Specificity: human, mouse, rat IF : HeLa cells, HepG2 cells, MCF-7 cells Cited Species: human, rat The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric Background Information protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3- p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation. Notable Publications Author Pubmed ID Journal Application Haochen Wang 33131748 Life Sci WB,IHC Furong Huang 32200035 Cancer Lett WB Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data K-562 cells were subjected to SDS PAGE followed IP result of anti-PRKAR2A (10142-2-AP for IP and Immunofluorescent analysis of HeLa cells using by western blot with 10142-2-AP (PRKAR2A Detection). 10142-2-AP (PRKAR2A antibody) at dilution of 1:25 antibody) at dilution of 1:1000 incubated at room and Alexa Fluor 488-conjugated AffiniPure Goat temperature for 1.5 hours. Anti-Rabbit IgG(H+L)..