See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/319876431 Parasitofauna isolated from fish off the east Algerian coast Article in Bulletin- European Association of Fish Pathologists · January 2017 CITATIONS READS 0 150 6 authors, including: Dalia Hafir Mansouri Zouhir Ramdane Université de Béjaïa Université de Béjaïa 3 PUBLICATIONS 0 CITATIONS 27 PUBLICATIONS 146 CITATIONS SEE PROFILE SEE PROFILE Halim Hafir Rachid Amara Directorate General of Forests Université du Littoral Côte d'Opale (ULCO) 3 PUBLICATIONS 0 CITATIONS 160 PUBLICATIONS 2,500 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: improves knowledge on Algerian fish parasitofauna View project improves knowledge on Algerian fish parasitofauna View project All content following this page was uploaded by Zouhir Ramdane on 18 September 2017. The user has requested enhancement of the downloaded file. ŗŚŞǰȱǯȱǯȱǯȱȱǯǰȱřŝǻŚǼȱŘŖŗŝ ȱȱȱęȱ ěȱȱȱȱ ǯȱ ęȬ1*, Z. Ramdane1, N. Kadri2,3, ǯȱ ę4, J-P. Trilles5 and R. AmaraŜ 1Laboratoire de Zoologie Appliquée et d’Ecophysiologie Animale, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie ; 2Laboratoire de Biochimie, Biophysique, Biomathématiques et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algérie ; 3Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Bouira, 10000 Bouira, Algérie ; 4Direction Générale des Forêts, Chemin Doudou Mokhtar, B.p. 232, Ben Aknoun, Alger, Algérie ; 5Équipe Adaptation Eco Physiologique et Ontogenèse, Université de Montpellier, CC. 092, Place E. Bataillon, 34095 Montpellier cedex 05, France ; 6·ȱĴȱȱȂǰȱ·ȱǰȱǰȱȱ 8187, LOG, Laboratoire d’Océanologie et de Géosciences, F62930 Wimereux, France Abstract ȱ ¢ȱŘŖŗŗȱȱ ¢ȱŘŖŗřǰȱȱȱŗŜŚřȱǰȱȱȱȱ ȱ ȱǻƽŗśŚśǼȱȱȱǻƽşŞǼȱ ȱǯȱȱŗŚȱęȱǰȱŗŝȱȱȱ ȱ ǰȱȱ ȱȱȱȱȱ ȱȱǯȱȱȱ ȱȱ rate, some isolated parasite species induce serious necrosis, alterations and atrophies to the muscle, ȱ¢ȱȱȱȱȱȱ¢ȱPyroderma cylindricum (P=9.37%; I=1); Lerneolophus sultanus (P=9.09%; I=1), and Ceratothoa oestroidesȱǻƽŖǯŜŘƖDzȱ ƽŗǼǰȱ¢ǯȱȱȱěȱ ȱ ȱȱȱęȱȱ¢ȱNaeobranchia sygniformis (P=27.27%; I=1). Among the most abundant isolated parasites was the nematode, Anguillicola crassusȱǻƽŚŘǯśřƖDzȱ ƽŜǯŗŗǼȱȱȱ European eel, Anguilla anguillaǰȱ ȱȱȱȱĚ¢ȱȱȱȱ Ȭǯȱ ȱȱ¢ȱȱȱ ȱȱȱęȱǯ Introduction ǰȱŘŖŖŞǰȱŘŖŗŖǰȱŘŖŗŘDzȱȱȱǯǰ 2007, ȱȱȱȱȱȱ¢ȱ 2009, 2013; Ider et al., 2014; Ichalal et al., 2015). ecosystem (Zander et al., 2002), greatly contrib- ĴȱĴȱȱȱȱȱȱȱ uting to overall aquatic biodiversity. endangered species such as the European eel, Anguilla anguilla ǻǯǰȱŗŝśŞǼȱǻAnguilla; Anguil- ȱȱȱȱȱęȱ Ǽǯȱȱ¡ȱȱȱ¢ȱȱȱ ȱȱȱ¡¢ȱǻȱȱ ȱ¡ȱȱ ȱǻȬ Ǽȱǻȱȱǯǰȱ * Corresponding author’s email: [email protected] Bull. Eur. Ass. Fish Pathol., 37(4) 2017, 149 ŘŖŖşǼȱ ȱȱȱȱȱMyxi- ȱǻǼȱǻřǰȱƽŗŚşȱǼȱȱȱȱ dium giardi ǻ·¸ǰȱŗşŖŜǼȱǻMyxidiumDzȱ¢¡- ȱŗŜŚřȱęȱȱȱǻȱŘŖŗŗȱȱ diidae),ȱȱȱȱ¢ȱȱǻǼȱ ŘŖŗřǼǯȱȱęȱ ȱȱȱȱ (Boudjadi et al., ŘŖŖşǼȱȱȱȱ ǰȱ ȱ ȱęȱȱȱ ȱȱȱ ȱ by Anguillicola crassus (Kuwahara, Niimi and ¢ȱȱȱȱęȱ Hagaki, 1974) (Anguillicola; Dracunculidae). ȱȱȱęȱȱȱǯȱȱęȱ ȱȱȱ ȱȱȱȱ ȱȱȱȱ¢ȱ ȱȱȱęȱ ęȱęȱǯȱȱ ȱȱȱ ȱ¡ȱȱȱȱȱȱȱ a transparent, labelled plastic bag (date, study ęǰȱȱȱ ȱȱȱȱ area, etc.) and transported immediately (in less environments, and try to assess their potential ȱŚȱȱȬęǼȱȱȱ¢ȱȱȱǯȱ ȱěȱȱȱǯ ȱȱ ȱęȱȱȱȱ the nearest 1 mm using an ichtyometer, and Material and Methods total weight (Wt) was determined to the nearest ȱ¢ȱ ȱȱȱȱěȱ- Ŗǯŗȱȱȱȱȱǯȱȱ¡ȱ ȱ ȱȱȱȱȱȱȱǻȱŗǼDzȱ ȱ¢ȱȱȱȱ £ěȱęȱȱǻŗǰȱƽȱşŞȱǼǰȱ ȱ ǯȱȱ¢ǰȱęǰȱȱ¢ǰȱȱ ȱȱǻŘǰȱƽŗřşŜȱǼȱȱȱ ǰȱȱȱ Ȭȱ ȱ¡- Figure 1. Study area and sampling stations, 1:ȱ£ěȱęȱDzȱ2:ȱ ȱȱDzȱ3: Soummam River. 150, Bull. Eur. Ass. Fish Pathol., 37(4) 2017 ȱȱȱȱȱȱȱȱ- Caligus ligusticus ǻǰȱŗşŖŜǼȱǻCaligus; Caligi- secting stereomicroscope. Collected parasites dae); Gotocotyla acanthus (Parona et Perugia, ȱę¡ȱȱŝŖƖȱȱȱȱęǯȱ ŗŞşŜǼ (Gotocotyla; Gotocotylidae). The European Parasitological parameters (Prevalence (P %) eel, Anguilla anguillaȱȱȱȱȱ ƽȱȱȱȱȱȦȱȱȱȱ ȱ ȱȱȱȱȱ£ȱIchtyoph- ¡ȱȱƼȱŗŖŖDzȱȱ ¢ȱǻ Ǽȱƽȱȱ ȱęȱǻǰȱŗŞŝŜǼȱǻIchtyophtirius; ȱȱȱȱȱȦȱȱȱ Hymenostomatida). One nematode Anguil- ȱȱDzȱȱȱȱǻǼȱ licola crassusȱȱȱȱȱ ƽȱȱȱȱȱȱȱȦȱȱ ȱȱȱȱęȱȱȱȱ ȱȱ ȱȱ¡ȱǼȱ ȱȱ Bejaia, although have been already reported ȱȱȱȱǯȱǻŗşşŝǼǰȱȱȱȱ ȱȱȱDZȱMicro- which are summarised in Table 1. cotyle mugilisȱǻǰȱŗŞŝŞǼȱǻMicrocotyle; Micro- ¢Ǽȱȱȱȱǻȱ ęȱȱ ȱȱȱȱěȱȱȱ ǯǰȱŗşşŞǼǰȱPagellicotyle mormyriȱǻ£ǰȱŗŞŝŞǼȱ ȱȱǰȱ¢ȱȱȱȱȱA. crassus (PagellicotyleDzȱ¢Ǽȱȱ ȱȱ¢ǰȱ and Peroderma cylindricum ǻ ǰȱŗŞŜśǼȱǻPero- ȱǻǰȱŗşŜřǼȱȱȱǻǰŗşşśǼǰȱ derma; Pennellidae), a histological study was Atriaster heterodus ǻȱȱǰȱŗşŜşǼȱ ǯȱ ȱȱǻ Ȭȱȱ (AtriasterDzȱ¢Ǽȱȱ (Lebedev Ǽȱ ȱę¡ȱȱŗŖƖȱěȱ- ȱǰȱŗşŜşǼȱȱȱǻ£ȱȱ aldehyde solution, dehydrated in increasing Maillard, 1973), Polylabris tubicirrus (Paperna ethanol concentrations (70 to 95%), cleared in ȱ ǰȱŗşŜŚǼȱǻPolylabris; Microcotylidae) in ¡¢ȱȱĜȬǯȱ ȱ- ȱǻȱȱ ǰȱŗşŜŚǼǰȱȱǻ£ȱ ȱȱřΐȱȱǻȱȱȱ and Maillard,1973), Spain (Lopez-Roman, 1973) ȱȱȱǼȱ ȱȱ ȱȱȱȱȱǻ- using Leica RM2125 RTS rotary microtome. ȱȱ£ǰȱŗşŞşǼǰȱG. acanthus in Tunisia Sections were mounted on glass microscope ǻǰȱŗşşśǼȱȱA. crassus in Egypt (Koops ǰȱȱ ȱ¢Ȃȱ¡¢Ȭǰȱ ȱ ǰȱŗşŞşǼǰȱȱǻȱ ȱȱǯǰȱ ȱ ȱȱȱȱ¡ȱ ŗşşŜǼȱȱȱǻȱȱǯǰȱŗşşşǼǯȱ- by Leica DM1000 LED microscope. ȱȱȱ ȱȱȱ£ȱȱ ȱȱȱȱȱěȱȱȱ Results and Discussion the Mediterranean Sea. Diversity and infection rates of isolated parasites ȱȱȱ ȱęȱ Ceratothoa ȱęȱȱȱȱȱȱ oestroidesȱǻǰȱŗŞŘŜǼȱǻCeratothoa; Cymothoi- ęǯȱȱȱȱ ȱȱ dae) in the European pilchard Sardina pilchardus ęȱȱȱȱȱęǰȱ- (Walbaum, 1792) (Sardina; Clupeidae) and Nero- ȱŗŜȱ£ȱȱŗȱ£ȱ¡ǯȱ cila maculata ǻȱ ǰȱŗŞŚŖǼȱǻNerocila; Among the collected parasite species, three are Cymothoidae) in the tub gurnard Trigla lucerna new geographical records; Clavellisa emargi- ǻǯǰȱŗŝśŞǼȱǻTrigla; Triglidae).ȱȱ ȱęȱ nataȱǻ ¢ǰȱŗŞřŝǼ (Clavellisa; Lerneopodidae); ȱȱȱȱȱȱȱ Bull. Eur. Ass. Fish Pathol., 37(4) 2017, 151 Table 1.ȱȱȱȱȱȱȱ¡ǯ Lm Parasites Parasites Host Sites N (Min - Fs P% Am I groups species Max) 17.31 Sparus aurata 1 Şř (4.20 - // /000 ǻǯǰȱŗŝśŞǼ 31.50) Lernaeolophus sultanus Pr 9.09 0.09 1 ǻ ǰȱŗŞŜśǼ 27.75 Sparus aurata 211 (20.90 - Crustacea Naobranchia ǻǯǰȱŗŝśŞǼ 33.50) cygniformis Br 27.27 0.27 1 ǻ ǰȱŗŞŜřǼ Gnathia sp. Br, Bc 45.45 ŗŝǯŞŗ 39.2 Dicentrarchus ŗşǯŜś labrax 115 / / / 0 0 0 (7 - 32.10) ǻǯǰȱŗŝśŞǼ Lernanthropus kroyeri Br 33.33 0.33 1 Dicentrarchus řŘǯŘŜȱ (van Beneden, labrax 211 (30.30 - Crustacea ŗŞśŗǼ ǻǯǰȱŗŝśŞǼ 34) Caligus minimus ǻĴǰȱŗŞŘŗǼ Br 33.33 0.33 1 Caligus ligus- Sarpa salpa ȱŘŜǯř 230 Crustacea ticus Bc 3.33 0.033 1 ǻǯǰȱŗŝśŞǼ ǻŗŞȱȮȱřŘǼ ǻǰȱŗşŖŜǼȱȘȘ Peroderma cylin- ŗřǯŘŜ dricum Mus 9.37 0.09 1 Sardina pilchar- ǻ ǰȱŗŞŜśǼ dus 2 ŗŜŖ Crustacea (Walbaum, 1792) Ceratothoa oes- (10 - 20) troides Bc ŖǯŜŘ ŖǯŖŖŜ 1 ǻǰȱŗŞŘŜǼȱȘ Nerocila mac- Trigla lucerna 19.75 ulata 210 Crustacea ȱ 10 0.1 1 ǻǯǰȱŗŝśŞǼ ǻŗŜȱȮȱŘśǼȱ (Milne Ed- ǰȱŗŞŚŖǼȘ Lithognathus Pagellicotyle ŗŜǯŜŗ mormyrus 2 330 Monogenea mormyri Br 3.33 0.03 1 (14 – 22) ǻǯǰȱŗŝśŞǼ ǻ£ǰȱŗŞŝŞǼ Atriaster hetero- ŗŞǯşśȱ Boops boops dus 2 ŜŜŚ (10 - Monogenea Br ŜǯŖŘ 0.14 2.37 ǻǯǰȱŗŝśŞǼ (Lebedev et 27.50) ǰȱŗşŜşǼ 152, Bull. Eur. Ass. Fish Pathol., 37(4) 2017 Table 1 Continuedǯȱȱȱȱȱȱȱ¡ǯ Lm Parasites Parasites Host Sites N (Min - Fs P% Am I groups species Max) Atriaster hetero- dus Br ŗśǯŜŘ ŖǯŗŜ 1 (Lebedev et Diplodus vulgaris ŗŞ ǰȱŗşŜşǼ ǻ ě¢ȱȬ 2 ŜŚ Monogenea (14 – 29) ǰȱŗŞŗŝǼ Polylabris tubi- cirrus Br ŚǯŜş ŖǯŖŞ ŗǯŜŝ (Paperna et ǰȱŗşŜŚǼ Atriaster hetero- dus Br ŗŖǯŜŝ 0.11 1 (Lebedev et Diplodus cervinus ŘŜǯŜŝȱ ǰȱŗşŜşǼ 275 Monogenea ǻ ǰȱŗŞřŞǼ (10 - 55) Polylabris tubi- cirrus Br 4 0.07 ŗǯŜŝ (Paperna et ǰȱŗşŜŚǼ Gotocotyla acan- Diplodus annu- 12.44 thus laris 237 Monogenea Br ŗŜǯŘŘ 0.02 1.33 (10 - 17) (Parona et Peru- ǻǯǰȱŗŝśŞǼ ǰȱŗŞşŜǼȱȘȘ Gotocotyla acan- Lichia glauca ŘŞ thus 23 Monogenea Br 33.33 1.33 4 ǻǯǰȱŗŝśŞǼ ǻŘřȱȮȱřŜǼȱ (Parona et Peru- ǰȱŗŞşŜǼȱȘȘ Microcotyle Mugil cephalus 27.49 350 Monogenea mugilis Br 10 0.121.2 ǻǯǰȱŗŝśŞǼ (15 - 40) ǻǰȱŗŞŝŞǼ Anguillicola crassus Nematoda (Kuwahara, Sb 42.53 ŘǯŜ Ŝǯŗŗ řŜŗǯşş Anguilla anguilla Niimi and 3 Şŝ (197 - ǻǯǰȱŗŝśŞǼ Hagaki, 1974) ŞŘŘǼ Ichtyophtirius Protozoa ęȱ(Fou- 2.3 0.02 1 ǰȱŗŞŝŜǼ Alosa fallax alge- Clavellisa emar- 17.04 riensis (Regan, 312 Crustacea ginata (Kroyer, Br 25 0.25 1 (14 - 21) ŗşŖŜǼ ŗŞřŝǼȱȘȘ ȘDZȱ ȱȱȱȱDzȱȘȘDZȱ ȱȱȱȱDzȱDZȱȱȱ¡ȱęDzȱDZȱȱ ǰȱȬ¡DZȱȱȱ¡DzȱȱǻƖǼDZȱDzȱDZȱȱDzȱ DZȱȱ ¢Dzȱ DZȱ¡ȱDzȱDZȱȱ¢DzȱDZȱȱDzȱDZȱȱDzȱDZȱȱęDzȱDZȱȱęDzȱDZȱ Ȭ bladder ; Mus: Muscle; 1DZȱ££ȱęȱDzȱ2DZȱ ȱȱDzȱ3: Soummam River. Bull. Eur. Ass. Fish Pathol., 37(4) 2017, 153 ǯȱȱ¡ȱȱȱ ȱȱȱȱȱ the mean intensity reached 45.45% and 39.2 ȱȱȱȱȱǰȱ ȱ ȱȱȱǰȱ¢ǯȱȱ ȱȱȱȱȱȱ- ȱǰȱȱȱ ȱ¢ȱ ǯȱ ȱǰȱ ȱS. aurata and D. labrax ȱȱȱ¡ȱřřǯřřƖȱǻLernanthropus ȱȱ¢ȱȱȱȱȱ kroyeri, Caligus minimus and G. acanthus), with as Lernaeolophus sultanusȱǻ ǰȱŗŞŜśǼȱǻLernae- ȱ¡ȱȱȱȱA. crassus in the olophus; Pennellidae), Naobranchia cygniformis ȬȱȱA. anguillaȱǻƽŚŘǯśřƖDzȱ ƽŜǯŗŗȱ ǻ ǰȱŗŞŜřǼȱǻNaobranchia; Lernaeopodidae), ȱȱȱǼȱȱȱǰȱ Gnathia sp., Lernanthropus kroyeri (van Beneden, £ȱȱGnathiaȱǯȱȱȱȱȱȱ ŗŞśŗǼȱǻLernanthropus; Lernanthropidae), Caligus ¢ȱȱȱ ȱȱȱǰȱSparus minimusȱǻĴǰȱŗŞŘŗǼȱǻCaligus; Caligidae). aurata ǻǯǰȱŗŝśŞǼȱǻSparus; Sparidae) (P=45.45%; ƽřşǯŘȱȱȱȱǼǯȱ- ȱěȱȱȱ ȱȱȱȱȱȱȱ ȱȱȱȱęȱȱ ȱǯȱȬȱęȱȱ ¡ȱ ȱȬęDzȱȱȱȱ ȱĚȱȱȱ£ěǰȱS. aurata and organs were the gills and the oral cavity (Table the European sea bass, Dicentrarchus labrax ŗǼǰȱ ȱȱȱȱȱȱ ǻǯǰȱŗŝśŞǼȱǻDicentrarchus; Moronidae) did not has not been assessed in this study. Parasites ȱȱȱȱȱ ȱ ȱ ȱȱ ȱȱȱȬę¢ǰȱ ȱȱȱȱ ȱǯȱ ȱ¡ǰȱ¡ȱȱ¢¡ȱǻȱ This might be related to one or a combination ŘǼǯȱ¡ȱ ȱȱȱę¢ȱȱȱ- ȱȱ ȱDZȱȱȱȱ ȱ- ȱ ȱȱȱěǰȱ ȱ ȱęȱǻȱȱȱȱǼǰȱ observed both at macroscopic and microscopic indeed, the cages