UNIVERSITY OF CALIFORNIA ENVIRONMENT, HEALTH & SAFETY (EH&S) LABORATORY SAFETY DESIGN GUIDE Second Edition September 2007 University of California Industrial Hygiene Program Management Group TABLE OF CONTENTS INTRODUCTION.......................................................................................................................................v ACKNOWLEDGMENTS..........................................................................................................................vi 1. GENERAL REQUIREMENTS FOR LABORATORIES....................................................................1-1 A. Scope .................................................................................................................................1-1 B. Building Requirements .......................................................................................................1-1 C. Building Design Issues.......................................................................................................1-2 D. Laboratory Design Considerations.....................................................................................1-2 E. Hazardous Materials Design Issues...................................................................................1-5 F. Entries, Exits, and Aisle Width ...........................................................................................1-6 G. Electrical and Utility Issues ................................................................................................1-7 H. Accessibility........................................................................................................................1-8 I. Nonstructural Seismic Hazard Abatement .........................................................................1-8 J. Teaching Laboratories........................................................................................................1-9 2. ELECTRICAL SAFETY .....................................................................................................................2-1 A. Scope .................................................................................................................................2-1 B. General Design Guidelines ................................................................................................2-1 C. Pre-Commission Evaluation Acceptance...........................................................................2-5 3. LABORATORY VENTILATION AND FUME HOODS ......................................................................3-1 A. Scope .................................................................................................................................3-1 B. General Laboratory Ventilation Design Issues...................................................................3-1 C. Variable Air Volume (VAV) Systems..................................................................................3-3 D. Negative Pressurization .....................................................................................................3-4 E. Manifolding .........................................................................................................................3-5 F. Room Airflow/Pressure Control Devices and Control Systems .........................................3-6 G. Supply Air Arrangements ...................................................................................................3-7 H. Ducting ...............................................................................................................................3-9 I. Exhaust Fans and Systems .............................................................................................3-10 J. Building Discharge and Wind Engineering.......................................................................3-12 K. Emergency Ventilation .....................................................................................................3-14 L. Emergency Power............................................................................................................3-15 M. Hoods — Construction and Installation............................................................................3-15 N. Hoods — Face Velocities.................................................................................................3-18 O. Hoods — Sashes .............................................................................................................3-18 P. Hoods — Perchloric/Hot Acid Use ...................................................................................3-19 Q. Air Cleaning......................................................................................................................3-20 R. Specialty, Controlled Climate, and Cold Rooms ..............................................................3-21 S. Hoods — Commissioning and Performance Testing.......................................................3-22 4. EMERGENCY EYEWASH AND SAFETY SHOWER EQUIPMENT.................................................4-1 A. Scope .................................................................................................................................4-1 B. Applications ........................................................................................................................4-1 C. Equipment Requirements...................................................................................................4-3 ii D. General Location................................................................................................................4-3 E. Pre-Commissioning Testing ...............................................................................................4-4 F. Approved Equipment..........................................................................................................4-5 Appendix 4-1. Emergency Eyewash Safety Shower Commissioning Checklist......................4-6 Appendix 4-2. Combination Emergency Eyewash Safety Shower Illustration ........................4-7 5. PRESSURE VESSEL COMPONENTS AND SYSTEMS AND COMPRESSED GAS CYLINDERS5-1 A. Scope .................................................................................................................................5-1 B. Compressed Gas Cylinder Storage ...................................................................................5-1 C. Compressed Gas Cylinder Restraint..................................................................................5-3 D. Toxic and Corrosive Gas Storage and Distribution............................................................5-3 E. Requirements for Gas Cabinets.........................................................................................5-3 F. Monitoring Toxic and Highly Toxic Gases..........................................................................5-4 G. Silane .................................................................................................................................5-5 H. Design of Systems and Apparatus for Cryogenic Fluids....................................................5-6 I. Design of Pressure Vessels and Systems. ........................................................................5-7 6. HAZARDOUS MATERIALS STORAGE CABINETS........................................................................6-1 A. Scope .................................................................................................................................6-1 B. Approvals and Listings .......................................................................................................6-1 C. Design ................................................................................................................................6-1 D. Venting Hazardous Material Storage Cabinets....................................................................6-2 E. General Installation Requirements.......................................................................................6-3 7. BIOSAFETY LABORATORIES.........................................................................................................7-1 A. Introduction.........................................................................................................................7-1 B. Scope .................................................................................................................................7-1 C. Basic Laboratory Design for Biosafety Levels 1 and 2 Laboratories .................................7-2 D. Basic Laboratory Design for Biosafety Level 3 Laboratories .............................................7-5 Appendix 7-1. BMBL Requirements .......................................................................................7-20 Appendix 7-2. Biological Safety Cabinets...............................................................................7-23 8. ADDITIONAL REQUIREMENTS FOR RADIOACTIVE MATERIAL LABORATORIES...................8-1 A. Scope .................................................................................................................................8-1 B. Basic Laboratory Design ....................................................................................................8-1 C. Ventilation Considerations .................................................................................................8-2 D. Radioactive Material Waste Management .........................................................................8-2 9. ADDITIONAL REQUIREMENTS FOR LABORATORIES WITH IRRADIATORS AND/OR RADIATION-