Catalog # Aliquot Size S08-911-05 3 x 5 nmol S08-911-20 3 x 20 nmol S08-911-50 3 x 50 nmol SGK3 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # S08-911 Lot # Z2085-77 Specificity Formulation SGK3 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human SGK3 expression. shipped at room temperature. Product Description Reconstitution Protocol SGK3 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human SGK3 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_013257. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot CISK, SGKL 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or SGK1, Active S06-11G below -20oC. After resuspension, siRNA stock solutions ≥2 SGK2, Active S07-10G µM can undergo up to 50 freeze-thaw cycles without SGK3, Active S08-10G significant degradation. For long-term storage, it is SGK1, Unactive S06-15G recommended that the siRNA is stored at -70oC. For most SGK2, Unactive S07-14G favorable performance, avoid repeated handling and SGK3, Unactive S08-14G multiple freeze/thaw cycles. Anti-SGK1 S06-63R AKT (SGK) Substrate A08-58 Scientific Background Modified SGKtide S08-58B SGK3 is a member of the SGK family and encodes a SGKtide S08-58 phosphoprotein with a PX (phox homology) domain. PKD1 can phosphorylate and activate SGK3 in vitro (1). A 10-fold increase in PKD1 increases the phosphorylation status of SGK3. When expressed in human embryonic SGK3 siRNA Set I siRNA duplexes targeted against three exon regions kidney cells, IGF1 and peroxide significantly activate SGK3, and the activation could be reduced by Catalog # S08-911 preincubation with inhibitors of PI3 kinase. A yeast 2- Lot # Z2085-77 hybrid screen found direct interaction between human Packaging Specifications 2.5 nmol/tube for 3 x 5nmol SGK3 and GSK3. SGK3 phosphorylates several target proteins and has a role in neutral amino acid transport Format Lyophilized powder Stability 1yr at -70oC from date of shipment and activation of potassium and chloride channels (2). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is References recommended that the lyophilized and resuspended siRNAs are stored at 1. Kobayashi, T. et al: Characterization of the structure or below -20oC. After resuspension, and regulation of two novel isoforms of serum- and siRNA stock solutions ≥2 µM can glucocorticoid-induced protein kinase. Biochem. J. undergo up to 50 freeze-thaw cycles without significant degradation. For 344: 189-197, 1999. long-term storage, it is recommended 2. Gamper, N. et al: K+ channel activation by all three that the siRNA is stored at -70oC. For isoforms of serum- and glucocorticoid-dependent most favorable performance, avoid protein kinase SGK. Europ. J. Physiol. 445: 60-66, 2002. repeated handling and multiple freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .