GABPB2 Antibody Cat. No.: 45-646 GABPB2 Antibody 45-646 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed 45-646 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, antigen retrieval with citrate buffer pH 6, AP-staining. AP-staining. 45-646 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. 45-646 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, Primary incubation 1hr (10ug/ml) permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed followed by Alexa Fluor 488 secondary by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. antibody (2ug/ml), showing Actin filaments were stained wit nuclear/nuclear speckle staining. Actin filaments September 28, 2021 1 https://www.prosci-inc.com/gabpb2-antibody-45-646.html 45-646 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog IMMUNOGEN: The immunogen for this antibody is: C-SSENSSKATDETG TESTED APPLICATIONS: ELISA, Flow, IF, IHC, WB Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2, NP_001307844.1 and of 38.1kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22kDa and was successfully blocked by incubation with the immunising peptide. Recommended concentration: APPLICATIONS: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. SPECIFICITY: #To_Delete#. POSITIVE CONTROL: 1) Cat. No. 1201 - HeLa Cell Lysate Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated September 28, 2021 2 https://www.prosci-inc.com/gabpb2-antibody-45-646.html PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: GABPB2 GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein ALTERNATE NAMES: transcription factor, beta subunit 1, 53kDa, GA binding protein transcription factor, beta subunit 2, 47kDa, nuclear respiratory factor-2 subunit beta ACCESSION NO.: NP_005245.2, NP_002032.2, NP_001307844.1 PROTEIN GI NO.: 8051593 GENE ID: 2553 Background and References 1) O'Leary DA, Koleski D, Kola I, Hertzog PJ, Ristevski S. Identification and expression REFERENCES: analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3;344:79-92. Epub 2004 Nov 23. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 3 https://www.prosci-inc.com/gabpb2-antibody-45-646.html.