Consellería do Mar Centro de Investigacións Mariñas Annual report 2008 Technical data Edit XUNTA DE GALICIA Consellería do Mar CIMA (Centro de Investigacións Mariñas) Coordination Alejandro Guerra (CIMA) Composing IMPRONTA GRÁFICA, s.l Printing LUGAMI A.G., s.l. Dep. Legal C-2747-2009 Translation Andrea Martínez Beiras Annual report 2008 Centro de Investigacións Mariñas Index 13 1.- INTRODUCTION 17 2.- CENTRO DE INVESTIGACIÓNS MARIÑAS (CIMA, Marine Research Centre) 19 2.1. STRUCTURE AND FUNCTION 20 2.1. CIMA STAFF 2.2.1. STAFF EMPLOYED AT THE CIMA OF VILANOVA DE AROUSA 2.2.2. STAFF EMPLOYED AT THE MARINE CULTURES CENTRE OF RIBADEO 24 2.3. RUNNING EXPENSES OF THE CIMA 2.3.1. CIMA’S EXPENSES I.- DISTRIBUTION OF CIMA’S BUDGET BY CHAPTERS II.- SOURCE OF FUNDS TO FINANCE THE INVESTIGATION 25 3.- SCIENTIFIC ACTIVITY 27 3.1. SUMMARIES OF THE RESEARCH PROJECTS A.- PROJECTS IN WHICH THE CHIEF RESEARCHER BELONGS TO THE CIMA 3.1.1 AREA OF AQUACULTURE PROJECTS -Culture and management of the perlemoen abalone (Haliotis tuberculata spp). -Reuse of wastewater of marine and auxiliary culture establishments located in terrestrial area. -Culture of new species of bivalve molluscs of interest in hatcheries. -Towards an integrated management of Ensis stocks (TIMES). -ALBA: Improvement of the pullet carpet shell clam (Venerupis pullastra, Montagu, 1803). -Optimization of the shell clam intensive culture and identification of the genetic markers for the follow up of the res- tocking. -Culture and management of the sea urchin (Paracentrotus lividus, Lamarck, 1816). Subproject I A: Determination of the reproductive cycle and biochemical composition of gonads of the sea urchin Paracentrotus lividus, Lamarck, 1816, in natural populations of Galicia. Culture of the sea urchin in laboratory: production of juveniles for repopulation. -Proposals and improvements for the design and control of the restocking plans and evaluation of the impact of esca- pes of fish and crustaceans. -Integrated Aquaculture: Experimental experience for the development of multitrophic culture system. -Optimization of the octopus (Octopus vulgaris) grow-out. Compound feed development (Galicia). ACTIONS -Prevention and protection study techniques of the principal predator species of the natural shoals bivalve molluscs’ resources and culture areas at the Galician coast and estuaries. -Integral microbiological control in molluscs hatcheries. -Study of operability of the tubort (Psetta maxima) restocking and the European Lobster (Homarus gammarus) at the Coast of Galicia. -Start-up and validation of a grow-out system of clam at the pontoon of the dock of O Vicedo (Lugo). -Follow-up and validation of mollusc minihatcheries. -Grow-out and culture of Queen Scallops (Aequipecten opercularis) and King Scapllop (Pecten maximus). 9 3.1.2. AREA OF PATHOLOGY PROJECTS -Offsprings production of the corresponding seeds to F3 Generation, in a program of genetic selection to produce a stock of flat oyster Ostrea edulis tolerat to the bonamiosis and with a high rate of survival. -Gene identification of resistance/susceptibility as potential indicators of disease in the flat oyster (Ostrea edulis) and in the Pacific (Crassostrea gigas) -Neoplasic disease in marine bivalves of commercial interest. -Perkinsosis in the Spanish Coast: characterization of the taxonomic variants of the parasite, of its live cycle, and its immunological answer to the host. ACTION -Tumour alterations that affect the golden carpet shell, the flat oyster, and the cockle in Galicia. Characterization of the disease and effects on the populations. 3.1.3. AREA OF COASTAL OCEANOGRAPHIC PROCESSES PROJECTS -Miltids Culture: expansion and sustainability. Subproject: Evaluation of the impact of the methods and levels used for the toxin control in mussels. -Reduction of the type PSP and DSP toxins incorporation in the rafts of mussels. -Comparison of methodologies for the determination of paralyzing toxins in bivalves related to the Paralytic Shellfish Poisoning (PSP). Uses in Spanish aquaculture. Subproject: Comparison of methodologies for the determination of paralyzing toxins in bivalves related to the Paralytic Shellfish Poisoning (PSP). Uses in Galician aquaculture. ACTIONS -Investigation action about toxicological episodes of phytoplankton: in bivalve molluscs found in Galicia. (EPITOX Action). Sub Project 6- Ligaments of the DSP toxins in the cells of the mollusc, acceleration of the elimination of the toxins and the ASP through non digestible substances and analytic aid towards the epi- demiology and genetic sub projects. -Toxic episodes of phytoplankton origin in the bivalve mollusc of Galicia. Sub project 8: Production of fami- lies of the mussels in hatcheries for genetic studies. tóxicos de origen fitoplanctónico en los moluscos bival- vos de Galicia. Subproyecto 8: reducción en criadero de familias de mejillón para estudios genéticos 3.1.4. AREA OF MARINE RESOURCES PROJECTS -Incorporation fo immunodetection tecniques in the monitorization of the mussel larvae in Galician coastal waters. I. Adaptation of the sampling protocols and larva determination. -Study of the detoxification and grow-out of the King scallop Pecten maximus (Linnaeus, 1758) in the Vigo Ria. -King Scallop Phase 1: Study of the stock populations of King Scallop (Pecten maximus, L., 1758) in Ares, - Arousa and Vigo Rias. -King Scallop Phase 2: Evaluation of the stock populations of King Scallop in Pontevedra, Muros and O Ferrol Rias and study of the dynamic of populations in Vigo and Ares Rias. ACTIONS -Geographic Information System Oriented towards the Management of Marine Resources (SIGREMAR). -Action Plan for the recovery of the shellfish bed “Lombos do Ulla” and “O Bohído” (Arousa Ria). 10 87 B.- PROJECTS WITH PARTICIPATION OF RESEARCHERS AND/OR CIMA STAFF -IDENTILARVAS. Genetic characterization of species of bivalves’ mollusc and PCR in real time design systems for its application in the detection and identification of larvae in our planktons. -Optimization of industrial production techniques of the flat oyster (Ostrea edulis). -Action investigation about toxic episodes of phytoplankton origin in mollusc bivalves of Galicia (Epitox Action). Subproject 1. Epidemiological detection aspects of intoxications effect of cooking n toxicity and the size of the portion of bivalves. -Action investigation about toxic episodes of phytoplankton origin in the mollusc bivalves of Galicia (Epitox Action). Subproject 3. Improvement of the control system of bivalves affected by biotoxins through the develop- ment and implementation of dynamic models. -Analysis and depuration of lipophilic toxins, bitter domoic acid that allows the exportation of Chilean wedge clams (Mesodesma donacium) and clam (Mulinia edulis) to European markets from the Coquimbo Bay. -Exploration and follow up of harmful microalgae for the activity of culture of pectinide (II, III and IV Region). -SEMAUMEX: Developmenta of an automatic splitting system of the mussel. -Analysis of evidences of the impacts due to the climate change in Galicia. -Study and characterization of intracellular prokaryote Rickettsia type and other potntially pathogenous oxidati- ve bacterias for the clam. -Study of environmental factors and potential pathogens that affect the grooved carpet shell clam culture (Ruditapes decussatus) in the Pontevedra Ria. -Design and application of the recirculation systems of the larval and grow-out phase of the marine fish and con- tinental aquaculture. -Analysis and study of culture factors and conditions of industrial production of the Senegalese sole (Solea sene- galensis). Subproject: Improvement of the parameters in the reproduction of the Senegalese sole (Solea senega- lensis). -Pigment characterization and light regulation in the dynoflagellates. -Characterization of the quality of the breaded fish. Subproject: Characterization of the quality of the breaded fish. -Feasibility study of the culture of the Pacific Oyster or Giant Oyster (Crassostrea gigas) in the Galician Rias. 113 C.- PROJECTS AND RESEARCH LINES PROMOTED AND/OR FINANCED THROUGH THE DXIDP -Genetic markers for the identification of the “Mexillón de Galicia”. -INDAQUA: Selection of indicators, determination of reference values, design of programs and protocols, and mea- sures for environmental studies in marine aquaculture. -Sanitary Management in Aquaculture (GESAC). Adaptation to the new ground rules. Characterization and standar- dization of conditions of animal health in marine aquaculture: creation of epidemiologic maps and strategies of ela- boration for the design of a net for epidemiologic surveillance. -Adjustment of an intensive culture system of octopus paralarvea, Octopus vulgaris, and importance of the bioche- mical composition in the larval diet. 125 3.2. DOCTORAL THESIS AND ADVANCED STUDIES DEGREES (DEA) CARRIED OUT AT THE CIMA. 131 3.3. ADVISORY SERVICES FOR THE SECTOR AND THE ADMINISTRATION 139 3.4. SCIENTIFIC AND TRAINING ACTIVITIES CARRIED OUT BY THE STAFF 3.4.1. ORGANIZATION, CHAIRMANSHIP AND PARTICIPATION IN SCIENTIFIC MEETINGS 3.4.2. DIRECTION OF DOCTORAL THESES AND INTERN TRAINING 3.4.3. COURSES GIVEN 3.4.4. PARTICIPATION ON EXAMINING BOARDS TO JUDGE FOR PHDs, DIPLOMAS OF ADVANCED STUDIES AND MAS- TER’S DEGREES 11 3.4.5. EDITION OF SCIENTIFIC PUBLICATIONS 3.4.6. REVIEW OF ARTICLES IN SCIENTIFIC PUBLICATIONS 149 3.5. STAFF TRAINING 3.5.1. VISITS TO OTHER CENTRES 3.5.2. COURSE ATTENDANCE 152 3.6. COLLABORATION AGREEMENTS 153 3.7. OUTSIDE VISITORS 154 3.8. PUBLICATIONS 3.8.1. ARTICLES IN JOURNALS