CRTC2 Antibody Cat. No.: 7325 CRTC2 Antibody Immunohistochemistry of CRTC2 in human small intestine tissue with CRTC2 antibody at 2.5 μg/ml. Immunofluorescence of CRTC2 in human small intestine tissue with CRTC2 antibody at 20 μg/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%) CRTC2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRTC2. IMMUNOGEN: The immunogen is located within the last 50 amino acids of CRTC2. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB September 28, 2021 1 https://www.prosci-inc.com/crtc2-antibody-7325.html CRTC2 antibody can be used for detection of CRTC2 by Western blot at 1 - 2 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Multiple isoforms of CRTC2 are known to exist. CRTC2 antibody is predicted to not cross- SPECIFICITY: react with CRTC1. POSITIVE CONTROL: 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide Predicted: 67, 76 kDa PREDICTED MOLECULAR WEIGHT: Observed: 70 kDa Properties PURIFICATION: CRTC2 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: CRTC2 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL CRTC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. Additional Info OFFICIAL SYMBOL: CRTC2 CRTC2 Antibody: TORC2, TORC-2, TORC2, CREB-regulated transcription coactivator 2, ALTERNATE NAMES: Transducer of regulated cAMP response element-binding protein 2 ACCESSION NO.: NP_859066 PROTEIN GI NO.: 32171215 GENE ID: 200186 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 28, 2021 2 https://www.prosci-inc.com/crtc2-antibody-7325.html CRTC2 Antibody: CRTC2, also known as TORC2 (Transducers Of Regulated cAMP Response Element-Binding (CREB)) 2 and the related protein CRTC1 are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner via phosphorylation- dependent interactions. Studies suggest that their phosphorylation and BACKGROUND: nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP. CRTC2 is present in both B and T-lymphocytes and abundantly expressed in the thymus. Its activity is important in regulating the expression of genes involved in cellular energy metabolism while CRTC1 is essential for energy balance and fertility. 1) Conkright MD, Canettieri G, Screaton R, et al. TORCs: transducers of regulated CREB REFERENCES: activity. Mol. Cell 2003; 12:413-23. 2) Iourgenko V, Zhang W, Mickanin C, et al. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells. Proc. Natl. Acad. Sci. USA 2003; 100:12147-52. 3) Bittinger MA, McWhinnie E, Meltzer J, et al. Activation of cAMP response element- mediated gene expression by regulated nuclear transport of TORC proteins. Curr. Biol. 2004; 14: 2156-61. 4) Jiang S, Inada T, Tanaka M, et al. Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1. Retrovirology 2009; 6:73. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 3 https://www.prosci-inc.com/crtc2-antibody-7325.html.