30 20 10 5 52 37 32 69 69 78 # mutations Non syn. Syn. A->T C->(A/T) A->(C/G) C->G indel+null double_null # mutations/Mb 0 1% 2% 1% 2% 1% 1% 1% 4% 1% 2% 1% 1% 2% 2% 1% 1% 1% 2% 1% 1% 1% 2% 2% 1% 2% 1% 3% 1% 1% 2% 2% 1% 2% 2% 2% 1% 1% 2% 1% 2% 1% 1% 2% 4% 2% 2% 1% 1% 2% 2% 1% 1% 2% 2% 1% 2% 2% 2% 2% 2% 1% 3% 2% 6% 3% 2% 1% 2% 2% 3% 3% 2% 2% 3% 2% 2% 2% 2% 2% 2% 2% 2% 2% 6% 2% 2% 2% 12% 2% 2% 3% 2% 3% 2% 3% 2% 7% 2% 2% 3% 5% 8% 3% 4% 3% 8% 3% 9% 9% 5% 9% 7% 11% 23% 20% 21% 0 1 2 3 4 GBM-06-0743-TP GBM-02-0003-TP GBM-32-1970-TP GBM-19-2625-TP GBM-06-2563-TP GBM-06-2567-TP GBM-06-0876-TP GBM-14-0813-TP GBM-28-5207-TP GBM-32-1991-TP GBM-76-4927-TP GBM-06-0157-TP GBM-06-0155-TP GBM-06-0649-TP GBM-06-0749-TP GBM-14-1395-TP GBM-76-6191-TP GBM-28-5209-TP GBM-06-2561-TP GBM-06-1804-TP GBM-06-0881-TP GBM-76-6661-TP GBM-14-1456-TP GBM-14-0790-TP GBM-06-6695-TP GBM-76-6656-TP GBM-26-5139-TP GBM-06-0750-TP GBM-81-5911-TP GBM-14-1034-TP GBM-27-2528-TP GBM-06-5413-TP GBM-06-0879-TP GBM-15-0742-TP GBM-19-1790-TP GBM-06-0154-TP GBM-06-0158-TP GBM-06-0211-TP GBM-06-0650-TP GBM-06-0142-TP GBM-28-1747-TP GBM-06-0137-TP GBM-14-1829-TP GBM-06-2557-TP GBM-14-1823-TP GBM-12-3652-TP GBM-14-0786-TP GBM-06-5856-TP GBM-06-0174-TP GBM-76-4926-TP GBM-27-1831-TP GBM-81-5910-TP GBM-12-3650-TP GBM-14-0789-TP GBM-06-0747-TP GBM-06-6700-TP GBM-19-2624-TP GBM-06-0878-TP GBM-16-1045-TP GBM-06-2565-TP GBM-76-6662-TP GBM-32-2634-TP GBM-06-0184-TP GBM-76-6286-TP GBM-06-5858-TP GBM-76-6283-TP GBM-27-1838-TP GBM-06-0875-TP GBM-06-0195-TP GBM-27-2519-TP GBM-76-4925-TP GBM-16-0846-TP GBM-76-6664-TP GBM-19-2629-TP GBM-06-0128-TP GBM-15-1444-TP GBM-76-4929-TP GBM-74-6573-TP GBM-06-6698-TP GBM-76-6663-TP GBM-32-2491-TP GBM-06-0130-TP GBM-06-2569-TP GBM-26-5133-TP GBM-06-5417-TP GBM-06-0221-TP GBM-32-4208-TP GBM-27-2521-TP GBM-14-4157-TP GBM-26-1442-TP GBM-41-5651-TP GBM-06-2559-TP GBM-06-0238-TP GBM-02-2485-TP GBM-76-6193-TP GBM-06-6694-TP GBM-41-2575-TP GBM-06-2558-TP GBM-32-4210-TP GBM-28-5219-TP GBM-27-1836-TP GBM-12-0619-TP GBM-06-0237-TP GBM-06-0744-TP GBM-06-0189-TP GBM-14-0817-TP GBM-28-5216-TP GBM-26-5136-TP GBM-14-0871-TP GBM-14-1825-TP GBM-06-0190-TP GBM-76-6657-TP GBM-26-6174-TP GBM-19-5955-TP GBM-32-4213-TP GBM-06-0192-TP GBM-41-4097-TP GBM-06-5412-TP GBM-06-0939-TP GBM-32-5222-TP GBM-12-1597-TP GBM-12-0618-TP GBM-06-0166-TP GBM-76-4931-TP GBM-06-0124-TP GBM-16-0861-TP GBM-28-2510-TP GBM-27-1834-TP GBM-06-0216-TP GBM-76-6192-TP GBM-06-0188-TP GBM-06-5418-TP GBM-27-1837-TP GBM-28-1753-TP GBM-19-5952-TP GBM-06-0125-TP GBM-02-2470-TP GBM-41-2573-TP GBM-76-4934-TP GBM-32-1977-TP GBM-06-0644-TP GBM-76-4935-TP GBM-06-0168-TP GBM-02-0055-TP GBM-26-5132-TP GBM-19-5959-TP GBM-74-6577-TP GBM-32-1982-TP GBM-41-2571-TP GBM-06-0882-TP GBM-74-6575-TP GBM-06-2562-TP GBM-06-0129-TP GBM-19-5947-TP GBM-28-2513-TP GBM-76-6280-TP GBM-06-6388-TP GBM-06-0877-TP GBM-41-3915-TP GBM-19-5951-TP GBM-12-0688-TP GBM-02-0033-TP GBM-32-2632-TP GBM-19-2623-TP GBM-06-0213-TP GBM-06-0140-TP GBM-27-2524-TP GBM-06-0214-TP GBM-06-5410-TP GBM-41-3392-TP GBM-14-1043-TP GBM-06-0119-TP GBM-28-6450-TP GBM-28-2502-TP GBM-19-5953-TP GBM-32-1986-TP GBM-41-6646-TP GBM-32-1980-TP GBM-28-5208-TP GBM-32-2494-TP GBM-06-2570-TP GBM-02-2483-TP GBM-06-6389-TP GBM-06-6701-TP GBM-06-6391-TP GBM-06-0210-TP GBM-27-1835-TP GBM-32-4211-TP GBM-06-0185-TP GBM-26-5135-TP GBM-28-5213-TP GBM-19-5954-TP GBM-02-0047-TP GBM-12-5301-TP GBM-41-2572-TP GBM-06-0171-TP GBM-74-6578-TP GBM-32-1979-TP GBM-16-1048-TP GBM-27-2527-TP GBM-06-5414-TP GBM-06-6390-TP GBM-27-2526-TP GBM-19-2620-TP GBM-02-2486-TP GBM-06-5411-TP GBM-28-5211-TP GBM-76-4928-TP GBM-12-0615-TP GBM-28-5214-TP GBM-06-0152-TP GBM-06-5408-TP GBM-06-5415-TP GBM-28-2514-TP GBM-12-3649-TP GBM-06-6697-TP GBM-26-5134-TP GBM-14-0781-TP GBM-28-2501-TP GBM-06-0169-TP GBM-06-6699-TP GBM-06-0122-TP GBM-19-2619-TP GBM-26-1439-TP GBM-19-5960-TP GBM-06-0646-TP GBM-76-6282-TP GBM-26-6173-TP GBM-06-0240-TP GBM-06-0145-TP GBM-14-0787-TP GBM-14-0862-TP GBM-32-4209-TP GBM-06-0141-TP GBM-06-0173-TP GBM-28-5204-TP GBM-19-1390-TP GBM-19-2631-TP GBM-06-0686-TP GBM-41-3393-TP GBM-12-5295-TP GBM-06-0209-TP GBM-14-0740-TP GBM-12-0821-TP GBM-76-6660-TP GBM-27-2523-TP GBM-28-5218-TP GBM-12-0692-TP GBM-14-2554-TP GBM-28-2509-TP GBM-27-1830-TP GBM-32-2495-TP GBM-14-1450-TP GBM-12-3653-TP GBM-06-0745-TP GBM-28-5220-TP GBM-76-4932-TP GBM-06-5859-TP GBM-06-0151-TP GBM-06-0126-TP GBM-19-4068-TP GBM-06-0645-TP GBM-32-2615-TP GBM-06-6693-TP GBM-06-0648-TP GBM-28-5215-TP GBM-06-0241-TP GBM-27-1832-TP GBM-32-2498-TP GBM-12-0616-TP GBM-87-5896-TP GBM-19-5950-TP GBM-06-0219-TP GBM-27-2518-TP GBM-06-0167-TP GBM-27-1833-TP GBM-14-3476-TP GBM-32-2638-TP GBM-06-2564-TP GBM-76-6285-TP GBM-19-5958-TP GBM-74-6584-TP GBM-12-5299-TP GBM-32-4719-TP GBM-06-1806-TP GBM-06-0132-TP GBM-28-2499-TP GBM-08-0386-TP GBM-06-0165-TP GBM-06-0139-TP GBM-06-0178-TP "OM121L12 %(!T1H2BE ADAMTS16 TMEM229B %EATR7B2 !ER"(#B4 DCA)12L2 .B! R17 Nonsense Splice site Missense Syn. TMPR!!6 NT#AP2 !LC$6A1 &GT2B28 TAS2R41 !ULT1B1 0 20 40 60 80 100 0 20 40 60 80 100 MAGEB4 "DG)RA "CD%A3 GABRB2 "CD%A1 GABRA1 DK#2C "RO'R2 GABRA6 !EMA3C $R5AR1 "OD#L1 $R52M1 TREML2 ADAM29 "CD%19 !PR,D5 LRR 55 OL1A2 $R5D18 &GT2A3 $R5D16 "LC*D3 ,"3A5 OL6A3 'DELC2 $R13C5 $R5D13 GLT1D1 !LC1A6 ALP"L2 $R5.2 "('3CA "OTE% !EMG1 %MC#1 D$ '5 $R5M3 "('3R1 ADC,5 AL R $R4D5 )$*R2 GNAT3 M& 16 M& 17 )$*G1 RBM47 TRP/6 !TAG2 DH18 $R5A1 ABCB1 AL#1 AZG"1 DC27 $R8'3 T!%+2 D""10 ELTD1 TRAT1 LRF#5 AP3!1 TPTE2 !PTA1 RIMS2 LZTR1 B $R 'LF17 GRM3 % .#T2 '()2B T %% EGFR TGFA DRD5 DSG3 #$*4 )GD5 "SG1 'PR" REL# % #1 "CL$ "!"% RYR2 "TE# "AN3 MSL3 "RB2 'LK6 RPL5 TP53 ID%1 LUM AFM REN )LG 'EL A1161ic #F1 RB1 -'( fraction 0.5 2.0 3.5 -log10(405alue) Other non syn. In frame in@61 Frame shift.