Catalogue # Aliquot Size S38-30EG-50 50 µg S38-30EG-200 200 µg SIRT4 Protein Full-length recombinant protein expressed in E. coli cells Catalog # S38-30EG Lot # M334 -4 Product Description Purity Full-length recombinant human SIRT4 was expressed in E. coli cells using an N-terminal GST tag. The gene accession number is NM_012240 . The purity was determined to be Gene Aliases >70% by densitometry. Approx. MW 62kDa . SIR2L4, MGC57437, MGC130046, MGC130047, sirtuin 4 Formulation Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Storage and Stability o Store product at –70 C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background SIRT4 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four SIRT4 Protein classes with SIRT4 being a member of class IV. SIRT4 lacks Full-length recombinant protein expressed in E. coli cells deacetylase activity but has ADP-ribosyltransferase Catalog Number activity (1). Immunoprecipitation analysis shows that SIRT4 S38-30EG interacts with the mitochondrial enzyme glutamate Specific Lot Number M334-4 dehydrogenase (GDH), and functional analysis show that Purity >70% SIRT4 ADP-ribosylates and inhibits GDH (2). Concentration 0.2µg/ µl Stability 1yr at –70 oC from date of shipment Downregulation of SIRT4 by RNA interference activates Storage & Shipping Store product at –70 oC. For optimal GDH, thereby upregulating insulin secretion in response to storage, aliquot target into smaller glucose and amino acids. quantities after centrifugation and store at recommended temperature. For References most favorable performance, avoid repeated handling and multiple 1. Frye, R. A. Characterization of five human cDNAs with freeze/thaw cycles. Product shipped on homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) dry ice. metabolize NAD and may have protein ADP- ribosyltransferase activity. Biochem. Biophys. Res. Commun. 260: 273-279, 1999. 2. Haigis, M. C. et al: SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. Cell 126: 941-954, 2006. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .