Milger et al. Pulmonary CCR2+CD4+ T cells are immune regulatory and attenuate lung fibrosis development Supplemental Table S1 List of significantly regulated mRNAs between CCR2+ and CCR2- CD4+ Tcells on Affymetrix Mouse Gene ST 1.0 array. Genewise testing for differential expression by limma t-test and Benjamini-Hochberg multiple testing correction (FDR < 10%). Ratio, significant FDR<10% Probeset Gene symbol or ID Gene Title Entrez rawp BH (1680) 10590631 Ccr2 chemokine (C-C motif) receptor 2 12772 3.27E-09 1.33E-05 9.72 10547590 Klrg1 killer cell lectin-like receptor subfamily G, member 1 50928 1.17E-07 1.23E-04 6.57 10450154 H2-Aa histocompatibility 2, class II antigen A, alpha 14960 2.83E-07 1.71E-04 6.31 10590628 Ccr3 chemokine (C-C motif) receptor 3 12771 1.46E-07 1.30E-04 5.93 10519983 Fgl2 fibrinogen-like protein 2 14190 9.18E-08 1.09E-04 5.49 10349603 Il10 interleukin 10 16153 7.67E-06 1.29E-03 5.28 10590635 Ccr5 chemokine (C-C motif) receptor 5 /// chemokine (C-C motif) receptor 2 12774 5.64E-08 7.64E-05 5.02 10598013 Ccr5 chemokine (C-C motif) receptor 5 /// chemokine (C-C motif) receptor 2 12774 5.64E-08 7.64E-05 5.02 10475517 AA467197 expressed sequence AA467197 /// microRNA 147 433470 7.32E-04 2.68E-02 4.96 10503098 Lyn Yamaguchi sarcoma viral (v-yes-1) oncogene homolog 17096 3.98E-08 6.65E-05 4.89 10345791 Il1rl1 interleukin 1 receptor-like 1 17082 6.25E-08 8.08E-05 4.78 10580077 Rln3 relaxin 3 212108 7.77E-04 2.81E-02 4.77 10523156 Cxcl2 chemokine (C-X-C motif) ligand 2 20310 6.00E-04 2.35E-02 4.55 10456005 Cd74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)16149 8.54E-07 3.37E-04 4.40 10444291 H2-Ab1 histocompatibility 2, class II antigen A, beta 1 14961 5.90E-06 1.11E-03 4.35 10463070 Entpd1 ectonucleoside triphosphate diphosphohydrolase 1 12495 1.24E-07 1.26E-04 4.32 10469278 Il2ra interleukin 2 receptor, alpha chain 16184 1.63E-08 3.22E-05 4.30 10415392 Ltb4r1 leukotriene B4 receptor 1 16995 2.11E-06 5.94E-04 4.22 10409866 Ctla2b cytotoxic T lymphocyte-associated protein 2 beta 13025 4.72E-06 9.80E-04 4.10 10526832 LOC100504914 transmembrane protein PVRIG-like 100504914 7.25E-07 2.99E-04 4.03 10355403 Fn1 fibronectin 1 14268 4.92E-04 2.06E-02 4.02 10389214 Ccl9 chemokine (C-C motif) ligand 9 20308 4.33E-06 9.33E-04 3.97 10444298 H2-Eb1 histocompatibility 2, class II antigen E beta 14969 4.98E-07 2.32E-04 3.70 10454015 Ttc39c tetratricopeptide repeat domain 39C 72747 2.62E-06 6.72E-04 3.59 10598292 Foxp3 forkhead box P3 /// RIKEN cDNA 4930524L23 gene /// coiled-coil domain containing 2220371 4.38E-10 8.26E-06 3.59 10356299 Gpr55 G protein-coupled receptor 55 227326 2.26E-05 2.60E-03 3.53 10394674 Socs2 suppressor of cytokine signaling 2 /// suppressor of cytokine signaling 2 pseudogene216233 6.55E-07 2.74E-04 3.49 10360070 Fcer1g Fc receptor, IgE, high affinity I, gamma polypeptide 14127 7.20E-04 2.66E-02 3.47 10368970 Prdm1 PR domain containing 1, with ZNF domain 12142 5.26E-08 7.64E-05 3.47 10409876 Ctla2a cytotoxic T lymphocyte-associated protein 2 alpha 13024 2.32E-07 1.63E-04 3.44 10378286 Itgae integrin alpha E, epithelial-associated 16407 3.63E-06 8.07E-04 3.43 10523182 Areg amphiregulin 11839 1.02E-05 1.55E-03 3.42 10603328 Ccdc22 coiled-coil domain containing 22 /// forkhead box P3 54638 6.91E-10 8.26E-06 3.41 10598004 Ccr1 chemokine (C-C motif) receptor 1 12768 8.18E-06 1.36E-03 3.38 10484894 Ptprj protein tyrosine phosphatase, receptor type, J 19271 1.05E-06 3.84E-04 3.37 10527638 Alox5ap arachidonate 5-lipoxygenase activating protein 11690 5.16E-05 4.45E-03 3.37 10420308 Gzmb granzyme B 14939 1.38E-04 8.80E-03 3.37 10345241 Dst dystonin 13518 1.34E-09 8.54E-06 3.32 10487506 Gm14005 predicted gene 14005 100043424 4.30E-05 4.00E-03 3.31 10486875 Frmd5 FERM domain containing 5 228564 2.41E-06 6.53E-04 3.31 10358816 Lamc1 laminin, gamma 1 226519 1.05E-07 1.19E-04 3.30 10415081 FJ556972 1.21E-04 8.09E-03 3.23 10520121 FJ556972 1.21E-04 8.09E-03 3.23 10578405 FJ556972 1.21E-04 8.09E-03 3.23 10586076 FJ556972 1.21E-04 8.09E-03 3.23 10363070 Gp49a glycoprotein 49 A 14727 5.97E-07 2.55E-04 3.22 10518300 Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b 21938 2.27E-07 1.63E-04 3.20 10421517 Cysltr2 cysteinyl leukotriene receptor 2 70086 6.52E-06 1.18E-03 3.18 10408935 Atxn1 ataxin 1 20238 3.56E-04 1.63E-02 3.18 10586781 Myo1e myosin IE 71602 4.86E-06 9.80E-04 3.15 10517508 C1qb complement component 1, q subcomponent, beta polypeptide 12260 5.35E-05 4.53E-03 3.13 10441436 Snx9 sorting nexin 9 66616 3.01E-07 1.71E-04 3.12 10355312 Ikzf2 IKAROS family zinc finger 2 22779 1.10E-06 3.85E-04 3.12 10569707 Myadm myeloid-associated differentiation marker 50918 3.41E-05 3.41E-03 3.11 10406270 Glrx glutaredoxin 93692 2.79E-07 1.71E-04 3.10 10587733 Ctsh cathepsin H 13036 2.05E-04 1.14E-02 3.10 10435704 Cd80 CD80 antigen 12519 2.60E-05 2.84E-03 3.10 10461558 Slc15a3 solute carrier family 15, member 3 65221 5.53E-07 2.46E-04 3.10 10466606 Anxa1 annexin A1 16952 2.63E-05 2.85E-03 3.08 10388591 Cpd carboxypeptidase D 12874 2.15E-06 5.94E-04 3.08 10569017 Ifitm3 interferon induced transmembrane protein 3 66141 3.87E-03 7.79E-02 3.08 10435907 Cd200r1 CD200 receptor 1 57781 1.89E-06 5.47E-04 3.07 10510580 Tnfrsf9 tumor necrosis factor receptor superfamily, member 9 21942 1.92E-05 2.32E-03 3.04 10405587 Tgfbi transforming growth factor, beta induced 21810 7.40E-05 5.86E-03 3.02 10539135 Capg capping protein (actin filament), gelsolin-like 12332 1.80E-05 2.22E-03 3.01 10580457 N4bp1 NEDD4 binding protein 1 80750 2.11E-05 2.51E-03 3.01 10588577 Cish cytokine inducible SH2-containing protein 12700 3.50E-07 1.88E-04 3.00 10521678 Cd38 CD38 antigen 12494 1.78E-04 1.04E-02 3.00 10509838 Padi2 peptidyl arginine deiminase, type II 18600 1.03E-05 1.56E-03 2.99 10346790 Ctla4 cytotoxic T-lymphocyte-associated protein 4 12477 1.70E-07 1.38E-04 2.98 10445977 Ebi3 Epstein-Barr virus induced gene 3 50498 1.55E-05 2.04E-03 2.95 10422728 Dab2 disabled homolog 2 (Drosophila) 13132 3.69E-04 1.67E-02 2.93 10586252 Dennd4a DENN/MADD domain containing 4A 102442 2.97E-07 1.71E-04 2.92 10597279 Ccrl2 chemokine (C-C motif) receptor-like 2 54199 5.43E-07 2.46E-04 2.90 10458581 Gm10008 predicted gene 10008 791268 9.40E-04 3.19E-02 2.90 10586240 Dennd4a DENN/MADD domain containing 4A 102442 2.09E-05 2.49E-03 2.88 10517513 C1qc complement component 1, q subcomponent, C chain 12262 2.08E-04 1.15E-02 2.88 10503334 Gem GTP binding protein (gene overexpressed in skeletal muscle) 14579 5.69E-04 2.27E-02 2.85 10355567 Tmbim1 transmembrane BAX inhibitor motif containing 1 69660 4.43E-05 4.07E-03 2.83 10401935 BC005685 cDNA sequence BC005685 352945 2.43E-04 1.26E-02 2.82 10381588 Grn granulin 14824 1.21E-05 1.70E-03 2.80 10590242 Ccr8 chemokine (C-C motif) receptor 8 12776 8.14E-05 6.26E-03 2.80 10542993 Pon3 paraoxonase 3 269823 1.35E-04 8.70E-03 2.79 10518313 Tnfrsf8 tumor necrosis factor receptor superfamily, member 8 21941 1.78E-03 4.83E-02 2.79 10371220 Gna15 guanine nucleotide binding protein, alpha 15 14676 2.11E-07 1.62E-04 2.78 10554863 Sytl2 synaptotagmin-like 2 83671 1.28E-05 1.76E-03 2.78 10547100 Plxnd1 plexin D1 67784 2.46E-07 1.67E-04 2.77 10379727 Gm11428 predicted gene 11428 100034251 6.09E-04 2.37E-02 2.75 10484888 Ptprj protein tyrosine phosphatase, receptor type, J 19271 3.49E-07 1.88E-04 2.75 10408937 Atxn1 ataxin 1 20238 3.34E-05 3.37E-03 2.75 10538901 BC005685 cDNA sequence BC005685 352945 3.39E-04 1.59E-02 2.74 10488589 Fam110a family with sequence similarity 110, member A 73847 2.42E-05 2.73E-03 2.74 10504757 BC005685 cDNA sequence BC005685 352945 1.26E-04 8.26E-03 2.74 10473281 Itgav integrin alpha V 16410 4.74E-06 9.80E-04 2.74 10387257 Alox8 arachidonate 8-lipoxygenase 11688 1.01E-06 3.72E-04 2.74 10389581 Ypel2 yippee-like 2 (Drosophila) 77864 4.12E-05 3.88E-03 2.74 10363082 Lilrb4 leukocyte immunoglobulin-like receptor, subfamily B, member 4 14728 8.98E-06 1.41E-03 2.71 10392142 Cd79b CD79B antigen 15985 1.38E-04 8.77E-03 2.71 10357833 Atp2b4 ATPase, Ca++ transporting, plasma membrane 4 381290 7.62E-07 3.10E-04 2.70 10544660 Osbpl3 oxysterol binding protein-like 3 71720 4.73E-06 9.80E-04 2.69 10603551 Cybb cytochrome b-245, beta polypeptide 13058 1.16E-05 1.65E-03 2.69 10413928 1810011H11Rik RIKEN cDNA 1810011H11 gene 69069 4.58E-04 1.96E-02 2.66 10560624 Apoe apolipoprotein E 11816 1.97E-04 1.11E-02 2.64 10401968 Galc galactosylceramidase 14420 3.90E-05 3.73E-03 2.64 10572897 Hmox1 heme oxygenase (decycling) 1 15368 2.84E-06 6.78E-04 2.64 10367436 Cd63 CD63 antigen 12512 2.84E-03 6.44E-02 2.64 10383756 Ifitm2 interferon induced transmembrane protein 2 80876 7.30E-04 2.68E-02 2.62 10493820 S100a6 S100 calcium binding protein A6 (calcyclin) 20200 1.90E-05 2.31E-03 2.62 10592515 Ubash3b ubiquitin associated and SH3 domain containing, B 72828 8.71E-10 8.26E-06 2.61 10569014 Ifitm2 interferon induced transmembrane protein 2 80876 2.38E-04 1.25E-02 2.61 10593050 Il10ra interleukin 10 receptor, alpha 16154 1.30E-06 4.09E-04 2.60 10482687 Arl5a ADP-ribosylation factor-like 5A 75423 7.60E-06 1.29E-03 2.60 10350733 Rgs16 regulator of G-protein signaling 16 19734 1.43E-07 1.30E-04 2.60 10409278 Nfil3 nuclear factor, interleukin 3, regulated 18030 2.14E-04 1.16E-02 2.58 10474064 Trp53i11 transformation related protein 53 inducible protein 11 277414 1.44E-07 1.30E-04 2.57 10414262 Ear2 eosinophil-associated, ribonuclease A family, member 2 /// cDNA sequence BC15109313587 1.77E-04 1.04E-02 2.56 10418341 Il17rb interleukin 17 receptor B 50905 3.85E-05 3.70E-03 2.56 10403229