Phylogeny of Alooideae TAXON Molecular and morphological analysis of subfamily Alooideae (Asphodelaceae) and the inclusion of Chortolirion in Aloe Barnabas H. Daru,! John C. Manning,"#$ James S. Boatwright,% Olivier Maurin,! Norman Maclean,& Hanno Schaefer,' Maria Kuzmina( & Michelle van der Bank! 1 African Centre for DNA Barcoding, University of Johannesburg, P.O. Box 524 Auckland Park 2006, Johannesburg, South Africa 2 Compton Herbarium, South African National Biodiversity Institute, Private Bag X7, Claremont 7735, South Africa Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa Department of Biodiversity and Conservation Biology, University of the Western Cape, Private Bag X17, Bellville 7535, Cape Town, South Africa School of Biological Sciences, University of Southampton, Highfield, Southampton, Hants, SO16 7PX, U.K. 6 Technische Universitaet Muenchen, Biodiversitaet der Pflanzen, Maximus-von-Imhof Forum 2, 85354 Freising, Germany International Barcode of Life Project, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario N1G 2W1, Canada Barnabas H. Daru, [email protected] Abstract matKrbcLa trnH-psbA Haworthia Astroloba Gasteria Astroloba H. RobustipeduncularesChortolirion Aloe Aloe A. aristata Haworthia Robustipedunculares Keywords matK rbcLa trnH-psbA Supplementary Material INTRODUCTION Aloe Gasteria AstrolobaHaworthia - - Aloe - Gasteria x - Haworthia Haworthia Astroloba Aloe- Chortolirion Lomato- phyllum Poellnitzia - - Lomatophyllum Aloe Poellnitzia Astroloba SansevieriaKniphofia AloeAstrolobaChortolirion 62 TAXON Phylogeny of Alooideae GasteriaHaworthia A. rubriflora Aloe Astroloba Astroloba - Astroloba Gasteria - Aloe Astroloba - Aloe Haworthia Poellnitzia rubriflora Astroloba Astroloba Haworthia Table . Aloe - Astroloba Chortolirion Gasteria Haworthia Phylogeny of Alooideae TAXON Haworthia- - Chortolirion - - Chortolirion Haworthia matKrbcLa trnH-psbA Chortolirion Aloe Aloe Leptoaloe Aloe MATERIALS AND METHODS Taxon sampling. — - matK rbcLatrnH-psbAGasteria - HaworthiaAloe Loma- tophyllum Astroloba Poellnitzia Chortolirion - Haworthia Gasteria . - - Anthericum liliago Haworthia Aloe Asphodeline lutea Bulbine fistu- losaB. frutescensB. semibar- Haworthia Haworthia bataEremurus spectabilisKniphofia Aloe H. Hexangu- galpiniiK. uvariaTeco- lares Gaste- philaea cyanocrocusZephyra elegans- ria Astroloba Aloe aristata Astroworthia Xanthorrhoea resinosaXanthorrhoea Astroloba Haworthia Chortolirion AloeLeptoaloeLomatophyllum Aloe.- - Haworthia H. Haworthia Hexangu- AloeGasteria laresRobustipedunculares Haworthia Haworthia . DNA extraction, amplification and sequencing. — TAXON Phylogeny of Alooideae Fig. .A, Aloe dichotoma B, A. perfoliata C, A. plicati- lis D, A. ferox E, A. spicata F, Haworthia chloracantha G, H. chloracantha H, Gasteria obliqua. — Phylogeny of Alooideae TAXON - Phylogenetic analyses and tree construction. —- - matK rbcLa trnH-psbA - trnH-psbA - - matK rbcLa - trnH-psbA - matK rbcLa - matK- - rbcLa trnH-psbA matK - - - - - matKrbcLatrnH-psbA - - - 66 TAXON Phylogeny of Alooideae - matK rbcLa trnH-psbA) Coding of morphological characters.