Monoclonal Anti-Syntrophin, clone 1351 produced in mouse, purified immunoglobulin Catalog Number SAB4200213 Product Description Reagent Monoclonal Anti-Syntrophin (mouse IgG1 isotype) is Supplied as a solution in 0.01 M phosphate buffered derived from the hybridoma 1351 produced by the saline, pH 7.4, containing 15 mM sodium azide. fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with Torpedo electric organ Antibody concentration: ~ 1.0 mg/mL membranes.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents, Precautions and Disclaimer Catalog No. ISO2. The antibody is purified from culture This product is for R&D use only, not for drug, supernatant of hybridoma cells grown in a bioreactor. household or other uses. Please consult the Material Safety Data Sheet for information regarding hazards Monoclonal Anti-Syntrophin recognizes human, mouse, and safe handling practices. rat, chicken, frog and xenopus Syntrophin. The antibody may be used in several immunochemical Storage/Stability techniques including immunoblotting (58 kDa), For continuous use, store at 2-8°C for up to one month. immunoprecipitation, immunofluorescence and For extended storage, freeze at -20oC in working 1 immunogold electron microscopy. aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight a1-Syntrophin (dystrophin-associated protein A1, 59 turbidity occurs upon prolonged storage, clarify by kDa, acidic component) is a member of the syntrophin centrifugation before use. Working dilution samples family, a biochemically heterogeneous group of 58-kDa should be discarded if not used within 12 hours. intracellular membrane-associated dystrophin-binding proteins. Several syntrophin isoforms (a1, b1, b2, g1, and Product Profile g2) have been identified in the heart, skeletal muscle, Immunoblotting: a working antibody concentration of and in neurons. Syntrophins are composed of four 0.5-1.0 mg/mL is recommended using whole extracts of conserved domains, two pleckstrin homology (PH) rat heart or L8 cells. domains, a PDZ domain and a highly conserved carboxyl-terminal syntrophin unique (SU) domain. a1- Note: In order to obtain the best results using various Syntrophin is mainly found at acetylcholine receptor techniques and preparations, we recommend (AchR)-rich regions of the postsynaptic membrane in determining optimal working dilutions by titration. Torpedo electrocytes and at AchR clusters on cultured muscle. Syntrophins bind directly to the C-terminal References domain of dystrophin, a large rod-like cytoskeletal 1. Froehner, S.C., et al., J. Cell Biol., 104, 1633- protein with an actin-binding domain, found at the inner 1646 (1987). surface of muscle fibers. Dystrophin is missing in 2. Tinsley, J.M., et al., Proc. Natl. Acad. Sci. USA, Duchenne Muscular Dystrophy patients and is present 91, 8307-8313 (1994). in reduced amounts in Becker Muscular Dystrophy 3. Ahn, A.H., et al., J. Biol. Chem., 271, 2724-2730 patients. Dystrophin is part of the dystrophin- (1996). glycoprotein complex (DGC), which bridges the inner 4. Wu, G., et al., Circ. Arrhythm. Electrophysiol., 1, cytoskeleton (F-actin) and the extra-cellular matrix. a1- 193-201 (2008). Syntrophin associates with the cytoplasmic face of the 5. Adams, M.E., et al., J. Biol. Chem., 270, 25859- DGC at the sarcolemma.1-6 25865 (1995). 6. Yang, B., et al., J. Biol. Chem., 269, 6040-6044 (1994). ST,RC,KAA,PHC 10/10-1 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip..