_____________________________________________________________________________ BREAKTHROUGHS IN REPRODUCTION AND DEVELOPMENT · NOUVELLES AVANCÉES EN REPRODUCTION ET DÉVELOPPEMENT Research Day Centre for the Study of Reproduction (CSR) at McGill Tuesday, May 19, 2015 McGill New Residence Hall 3625 Avenue du Parc Montréal, Québec ______________________________________________________________________________ BREAKTHROUGHS IN REPRODUCTION AND DEVELOPMENT Research Day 2015 Centre for the Study of Reproduction (CSR) at McGill Tuesday, May 19, 2015 New Residence Hall, 3625, avenue du Parc, Montréal, Québec 8:00-9:00 Registration and coffee / Poster set-up 9:00-9:10 Opening remarks: Dr. Daniel Bernard 9:10-10:10 Trainee oral presentations (Chairs: Steven Jones and Océane Albert) O-01. Lundi Ly, “Lifetime Folate Deficiency and Supplementation Induces Aberrant Sperm DNA Methylation and Reproductive Health” O-02. Rohini Bose, “Ubiquitin Ligase Huwe1 Modulates Male Germ Cell Development by Regulating Meiotic Progression” O-03. Stephany El Hayek, “Oocyte-Driven Remodeling of the Follicular Microenvironment Builds the Platform for Essential Germ Line-Somatic Communication” 10:10-10:40 Dr. Thomas Duchaine, Department of Biochemistry, McGill University, “What MicroRNA-Mediated Silencing in the C. elegans Embryo Can Tell Us About Cancer” – introduced by Dr. Loydie Jerome-Majewska 10:40-11:00 Health Break / Briefing of Judges 11:00-11:45 Dr. Mellissa Mann, Departments of Obstetrics & Gynecology, and Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, “Developmental Regulation of the Kcnq1ot1 Imprinted Domain” – introduced by Dr. Hugh Clarke 11:45-11:55 Introductory Remarks about Clermont Endowment Fund and Lectureship: Dr. Carlos Morales 11:55-12:45 Lunch 12:45-14:10 Poster Session P-01. Gauthier Schang, “Effects of Organophosphate Flame Retardants on Leydig Cell Function” P-02. Steven Jones, “Differential Effects of Combined Genistein and DEHP on Testicular Cell Lipid Homeostasis and Steroid Production” P-03. Anne Marie Downey, “Cyclophosphamide (CPA) Treatment Alters the Expression of Members of the Zip Family Zinc Transporters in Pachytene Spermatocytes” P-04. Karl-Frédéric Vieux, “The Cytoplasmic Deadenylase CNOT6 Regulates Deadenylation of a Subset of Transcripts During Oocyte Meiotic Maturation” P-05. Gurpreet Manku, “Acetaminophen Versus Ibuprofen: Effects on Neonatal Testicular Gonocyte Development” P-06. Paulo Roberto Antunes da Rosa, “EGFR Inhibition Prevents Meiotic Resumption in Bovine Oocytes Cocultured With Follicular Hemisections” P-07. Laleh Abbassi, “The Role of Yes-Associated Protein (YAP) in Regulation of Ocyte Growth” P-08. Johanna Selvaratnam, “The Effects of Catalase Overexpression on Developing Male Germ Cells in the Aged Mouse” P-09. Lorena Carvelli, “Heparin-Alpha-Glucosaminide N-Acetyltransferase (Hgsnat) Gene Inactivation Affects the Reproductive Tract of Adult Mice” P-10. Océane Albert, “Sperm Chromatin Quality Assessment: Optimization of the High Throughput Comet Assay” P-11. Enrique Gamero-Estevez, “Modifying the Claudin Binding Specificity of the C-Terminus of Clostridium Perfringens Enterotoxin (C-CPE)” P-12. Rodrigo Camponogara Bohrer, “Effects of Inhibiting DNA Repair Pathways During Early Embryo Development” P-13. Eskandari Shahraki Marzieh, “Knockout Mice Model to Study Functions of BSPH1 and BSPH2 in Fertility” P-14. Naomi Dicks, “Reduction of Endoplasmic Reticultum (ER) Stress by Treatment With Tauroursodeoxycholic Acid (TUDCA) Rescues Developmentally Incompetent Slow-Cleaving Porcine Embryos in Vitro” P-15. Suhaib Khayat, “Accumulation of Mitochondrial DNA During Early Murine Embryogenesis is Linked to the Resumption of Cell Growth” P-16. Aaron Kwong, “FGF4 as a Possible Regulator for Naïve to Primed Pluripotency Differentiation and Rossette Formation” P-17. Martika Rodgers, “The Role of Angiomotin in Hippo Signaling Regulation in the Early Mouse Embryo” P-18. Wenyang Hou, “Explants of Pre-Attachment Chorion and Allantois Reveal Extensive Mixing and a Requirement for the Allantois for Maintenance of Gcm1 and Tpbpα Expression” P-19. Yassemine Khawajkie, “Dissecting the Genetic Susceptibility to Sporadic Molar Pregnancies and Mechanisms of Their Formation” P-20. Elie Akoury, “NLRP7 and KHDC3L, the Two Maternal-Effect Proteins Responsible for Recurrent Hydatidiform Moles, Co-Localize to the Oocyte Cytoskeleton” P-21. Vafa Keser, “Genetic Models for Identifying the Molecular Basis of Phenotypic Variability in 22q11.2 Deletion Syndrome” P-22. Laura Whidden, “Combined Effects of DNA Methyltransferase 1o- Deficiency and Ovarian Stimulation on DNA Methylation Patterning and Embryonic Outcome at Mid-Gestation” P-23. Jonas Brandenburg, “Epigenetic Effects of Prenatal Exposure to Polycyclic Aromatic Hydrocarbons on CYP1A Expression and Inducibility” P-24. Keith Siklenka, “Over-Expression of Kdm1a in Spermatogenesis Alters the Sperm Epigenome and has Dire Consequences for Development of the Embryo” P-25. Kai Sheng, “Huwe1 Function on Male Fertility and Epigenetic Regulation During Spermiogenesis” P-26. Cécile Adam, “Understanding the Regulation of Connexin 26 in the Epididymis” P-27. Luisina Ongaro, “Gonadotrope-Specific Deletion of Bmpr2 Does Not Affect FSH Synthesis or Estrous Cyclicty in Adult Female Mice” P-28. Yasaman Aghazadeh, “Translocator Protein (TSPO) Drug Ligands Induce Testosterone Formation in GnRH Antagonist Castrated Rats” P-29. Nancy Li, “Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Transport for Steroid Biosynthesis” P-30. Sathvika Venugopal, “Cholesterol Trafficking for Steroid Biosynthesis in MA-10 Mouse Tumor Leydig Cells” P-31. Enrico Campioli, “Global Knockout and Steroidogenic Cell-Targeted Deletion of the Translocator Protein (18-kDa) Unveil its Crucial Role in Viability and Hormone-Dependent Steroid Formation” P-32. Yasmin Schuermann, “From the Dry to the Lactating Period: The Struggles of the Dairy Cow” P-33. Samin Sabouhi Zarafshan, “Mutations of Human Binder of Sperm Homolog1 (BSPH1): A New Cause of Male Infertility?” P-34. Swati Gupta, “Characterization of SEC23A and MAN1B1 Expression and Function in a Family With Craniofacial Abnormalities and Mental Retardation” P-35. Renata Bahous, “High Dietary Folate During Pregnancy and Lactation Leads to Disturbances in Folate Metabolism, Pseudo-MTHFR Deficiency and Short-Term Memory Impairment in Murine Offspring” P-36. Lisa-Marie Legault, “Implication of a Transient Kdm1a-Loss on the Embryonic Epigenetic Landscape” 14:10-15:10 Trainee oral presentations (Chairs: Laleh Abbassi and Enrico Campioli) O-04. Chirine Toufaily, “Addition of a C-Terminal Tail Alters Mammalian GnRH Receptor Signaling” O-05. Thomas Nardelli, “In Vitro Assessment of Novel Green Plasticizers Using a Toxicogenomic Approach in Immortalized Sertoli Cell Lines” O-06. Fatima Tokhmafshan, “Perturbations in the Extracellular Matrix of Developing Ureterovesical Junction May Lead to Vesicoureteral Reflux” 15:10-15:55 Dr. Jon Oatley, Director, Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University “Molecular Control of the Stem Cell State in Mammalian Spermatogonia” – introduced by Dr. Jacquetta Trasler 15:55-16:00 Concluding Remarks: Dr. Daniel Bernard 16:00-17:00 Cocktail / Awards Presentation / Take down posters NOUVELLES AVANCÉES EN REPRODUCTION ET DÉVELOPPEMENT Journée de recherche 2015 Centre d’études sur la reproduction (CER) à McGill le mardi 19 mai 2014 New Residence Hall, 3625, avenue du Parc, Montréal, Québec 8 h Inscription et café / Installation des affiches 9 h Mot de bienvenue : Dr. Daniel Bernard 9 h 10 Présentations orales (Modérateurs: Steven Jones et Océane Albert) O-01. Lundi Ly, “Lifetime Folate Deficiency and Supplementation Induces Aberrant Sperm DNA Methylation and Reproductive Health” O-02. Rohini Bose, “Ubiquitin Ligase Huwe1 Modulates Male Germ Cell Development by Regulating Meiotic Progression” O-03. Stephany El Hayek, “Oocyte-Driven Remodeling of the Follicular Microenvironment Builds the Platform for Essential Germ Line-Somatic Communication” 10 h 10 Dr. Thomas Duchaine, Department of Biochemistry, McGill University, “What MicroRNA-Mediated Silencing in the C. elegans Embryo Can Tell Us About Cancer”– présenté par Dr. Loydie Jerome-Majewska 10 h 40 Pause santé / Briefing des juges 11 h 00 Dr. Mellissa Mann, Departments of Obstetrics & Gynecology, and Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, “Developmental Regulation of the Kcnq1ot1 Imprinted Domain” – présentée par Dr. Hugh Clarke 11 h 45 Remarques introductives concernant le fond de dotation et conférencier Clermont: Dr. Carlos Morales 11 h 55 Dîner 12 h 45 Session d’affiche P-01. Gauthier Schang, “Effects of Organophosphate Flame Retardants on Leydig Cell Function” P-02. Steven Jones, “Differential Effects of Combined Genistein and DEHP on Testicular Cell Lipid Homeostasis and Steroid Production” P-03. Anne Marie Downey, “Cyclophosphamide (CPA) Treatment Alters the Expression of Members of the Zip Family Zinc Transporters in Pachytene Spermatocytes” P-04. Karl-Frédéric Vieux, “The Cytoplasmic Deadenylase CNOT6 Regulates Deadenylation of a Subset of Transcripts During Oocyte Meiotic Maturation” P-05. Gurpreet Manku, “Acetaminophen Versus Ibuprofen: Effects on Neonatal Testicular Gonocyte Development” P-06. Paulo Roberto Antunes da Rosa, “EGFR Inhibition Prevents Meiotic Resumption in Bovine Oocytes