Genetically engineered models (GEMS) p53 knockout rat Model p53 knockout rat Description Strain HsdSage:SD- Tp53tm1Sage SD Homozygous null Tp53 rats display onset of tumors at ~4 months of age. A high degree of malignancy is observed across a broad Location U.S. spectrum of tumors. Heterozygous rats have a delayed onset of Availability Cryopreserved spontaneous tumors, making them valuable for carcinogenicity screening, as well as studying efficacy of chemopreventive and therapeutic treatment. Characteristics/husbandry p53 is a tumor suppressor protein encoded by the Tp53 gene. + Monoallelic 11 base pair deletion in Tp53 Its role in cell cycle regulation and stabilization for preventing + Broad tumor spectrum genome mutation is observable among a wide variety of multicellular organisms, including humans, rodents, frogs, and + High degree of tumor malignancy fish. Heterozygous rats deficient in p53 protein are prone to + Background Strain: Sprague-Dawley spontaneous tumors, making them valuable for in vivo screening of carcinogenicity, as well as studying chemopreventive and Zygosity genotype therapeutic treatment. + Cryopreserved as heterozygous embryos Citations Research use Dai MS, Hall SJ, Vantangoli Policelli MM, Boekelheide K, Spade DJ. Spontaneous testicular atrophy occurs despite normal + Xenografts spermatogonial proliferation in a Tp53 knockout rat. Andrology. + Vaccine development 2017 Nov;5(6):1141-1152. + Cancer McCoy A, Besch-Williford CL, Franklin CL, Weinstein EJ, Cui X. + Autoimmune disease Creation and preliminary characterization of a Tp53 knockout rat. + Infectious disease Dis Model Mech. 2013 Jan;6(1):269-78. + Hematopoiesis Zungu-Edmondson M, Shults NV, Wong CM, Suzuki YJ. Modulators of right ventricular apoptosis and contractility in a rat Origin model of pulmonary hypertension. Cardiovasc Res. 2016 May 1;110(1):30-9. The p53 knockout rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo. North America 800.793.7287 EU and Asia envigo.com/contactus [email protected] envigo.com Figure 1. Weight and age comparison chart Figure 2. Survival rates of both heterozygous and homozygous HET male HET female HOM male p53 null rats (n = 30) 600 100.0 500 90.0 89.0 400 70.0 300 60.0 Weight grams Weight 50.0 200 Survival 40.0 100 30.0 Homozygous 20.0 Heterozygous 0 Wild Type 2 4 6 8 10 12 14 16 10.0 Age ees 0.0 0 50 100 150 200 250 300 350 Days Table 1. Tp53 (+/-) Tumor Spectrum Table 2. Tp53 (-/-) Tumor Spectrum Contact us North America 800.793.7287 [email protected] Envigo, 8520 Allison Pointe Blvd., Suite 400, Indianapolis, IN 46250, United States © 2020 Envigo..