TABLE 5. Proteins identified in Cumulus and Oocyte. GI Cell Protein DDF Protein name Peptides number typea categoryb fraction 30523262 100 kDa coactivator C K 5 2 2852383 14-3-3 protein beta C K 2 4 2852385 14-3-3 protein gamma C K 14 4 298639 155 kda myosin light chain kinase homolog O K 1 1 576 17,000 dalton myosin light chain CO K 5 3 8118661 17-beta-hydroxysteroid dehydrogenase type 1 C K 2 3 17864970 17-beta-hydroxysteroid dehydrogenase type 1 CO K 38 3 59857747 1-acylglycerol-3-phosphate O-acyltransferase 4 O K 1 3 30017445 2',5'-oligoadenylate synthetase 1, 40/46kDa C K 2 4,3 66792906 2'-5' oligoadenylate synthetase 2 C K 1 1 7271506 2-amino-3-ketobutyrate coenzyme A ligase O K 2 1 31414871 2-enoyl thioester reductase C K 2 4,1 45219953 5-hydroxytryptamine receptor 2A C K 2 4,1 47564115 5-oxo-L-prolinase CO K 5 1 34392345 90-kDa heat shock protein beta CO K 54 1 56967054 A Chain A, Crystal Structure Of Bosus Mitochondrial Elongation Factor TuT O K 2 3 27807141 A disintegrin-like and metalloprotease (reprolysin type) with thrombos O K 1 3 89579 A23707 aminomethyltransferase (EC 2.1.2.10) precursor C K 3 3 89381 A29600 alkaline phosphatase (EC 3.1.3.1) precursor, hepatic - bovine O K 1 1 108687 A36623 gap junction protein Cx43 C K 3 4 89346 A40981 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), cGMP-stimulated C K 1 4 1363022 A56351 cleavage and polyadenylation specificity factor 100K chain C K 1 3 1363051 A56534 interferon-induced double-stranded RNA-activated protein kinase inhibitor C K 1 4 14194421 AAKG1_BOVIN 5'-AMP-activated protein kinase, gamma-1 subunit C K 1 4 62751897 Abhydrolase domain containing 2 CO K 2 1 61554426 Abl-philin 2 isoform 2 CO K 2 3 63092048 Acetyl-CoA carboxylase, type beta C K 1 3 6006405 Acetyl-CoA-carboxylase C K 3 1 163596 Acid phosphatase C K 1 4 1649041 Acidic ribosomal phosphoprotein C K 2 1 2293577 Acidic ribosomal phosphoprotein PO C K 6 1 600177 Acidic ribosomal protein P2 C K 9 4 1351857 ACON_BOVIN Aconitate hydratase, mitochondrial precursor CO K 27 1 58013001 Actin [Cryptosporidium sp. ex Bosus] C K 5 4 61555662 Actin related protein 2/3 complex subunit 1B C K 1 2 27806765 Actin, beta CO K 20 2 1381584 Activin receptor type I C K 4 2 59857635 Acyl-Coenzyme A dehydrogenase, short/branched chain precursor C K 9 2 27806205 Acyl-coenzyme A dehydrogenase, very long chain C K 14 3 2498104 AD50_BOVIN ADRENAL MEDULLA 50 KD PROTEIN O K 1 2 20141178 ADA_BOVIN Adenosine deaminase (Adenosine aminohydrolase) O K 1 2 29420423 Adaptor related protein complex (AP)-3 delta subunit C K 2 4 57162630 Adaptor-related protein complex 3, mu 1 subunit C K 1 1 58760446 Adenine phosphoribosyltransferase C K 1 4 61888850 Adenylate kinase 1, soluble C K 1 4 217607 Adenylate kinase 2B CO K 18 1 895754 Adipocyte-type fatty acid binding protein C K 1 4 5823591 Adipophilin C K 2 3 13162341 ADP-ribosylation factor 2 C K 1 3 6680730 ADP-ribosylation factor-like 4 O K 1 1 3041655 ADRO_BOVIN NADPH:adrenodoxin oxidoreductase, mitochondrial precursor C K 3 4 52000728 ADT2_BOVIN ADP,ATP carrier protein 2 (ADP/ATP translocase 2) CO K 40 3 399012 ADT3_BOVIN ADP,ATP carrier protein, isoform T2 (ADP/ATP translocase 3) CO K 10 1 113469 ADX2_BOVIN Adrenodoxin 2, mitochondrial precursor (Adrenal ferredoxin) C K 1 4 31324244 Aggrecanase-1 OK 1 2 6319132 Aggrecanase-1 C K 1 4 6319134 Aggrecanase-2 C K 1 3 1352248 AL1A1_BOVIN Retinal dehydrogenase 1 (RalDH1) (RALDH 1) C K 1 4 162648 Albumin CO K 67 3 60302887 Aldose reductase C K 2 4 4097475 Allele: DQB*1004 C K 1 1 240434 Alpha 1C-adrenergic receptor [cattle, Peptide, 466 aa] C K 2 4,1 61553131 Alpha 2 actin CO K 69 2 386077 Alpha B-crystallin [cattle, lens, Peptide, 175 aa] C K 1 1 2967448 Alpha2(I) collagen C K 1 1 37652865 Alpha2,3-sialyltransferase O K 1 2 56292039 Alpha-2,6-sialyltransferase ST6Gal II C K 1 1 47564072 Alpha-2,8-sialyltransferase 8D C K 1 1 498822 Alpha-2-antiplasmin C K 1 3 61553826 Alpha-N-acetylgalactosaminidase precursor C K 2 4 2506821 AMBP_BOVIN AMBP protein precursor C K 1 4 525 Amine oxidase (flavin-containing); monoamine oxidase type A (MAO-A) C K 1 2 62751707 AminoadipateAminotransferase C K 1 2 4468135 Amyloid precursor protein O K 1 3 61555206 Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate C K 1 2 20455472 ANG1_BOVIN Angiogenin-1 precursor O K 1 3 12643645 ANGP2_BOVIN Angiopoietin-2 (ANG-2) C K 1 1 61555439 Ankyrin repeatAnd SOCS box-containing protein 14 C K 1 2 59858367 Annexin 5 C K 15 4 61554010 Annexin A8 C K 3 4,1 61553085 Annexin I C K 2 3 1842109 Annexin VI C K 25 2 1168458 ANPRB_BOVIN Atrial natriuretic peptide receptor B precursor (ANP-B) C K 1 2 432627 Anti-testosteroneAntibody CO K 4 3 113956 ANXA4_BOVIN Annexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) C K 10 4 113983 APEX1_BOVIN DNA-(apurinic or apyrimidinic site) lyase (AP endonuclease 1) CO K 7 2 162678 Apolipoprotein A-I precursor CO K 7 1 46359698 Apolipoprotein C-III C K 2 3 162680 Apolipoprotein H O K 1 3 42564208 Apoliprotein A-I binding protein O K 3 1 47681470 Apoptosis inhibitor survivin C K 1 3 26453359 Apoptosis-associated speck-like protein containing a CARD C K 1 3 114151 ARBK1_BOVIN Beta-adrenergic receptor kinase 1 (Beta-ARK-1) C K 1 4 19354187 Arf3 protein C K 2 4 46213277 Argonaute 3 C K 1 3 162642 Aromatic-L-amino acid decarboxylase C K 2 4 50978894 ARP1 actin-related protein 1 homolog A, centractin alpha C K 1 1 3004445 Arylacetyl acyl-CoA N-acyltransferase C K 1 1 3492930 Arylacetyltransferase C K 6 1 33359641 Aryl-hydrocarbon receptor-interacting protein O K 1 2 59858077 Aspartate aminotransferase 1 C K 1 4 27807467 Aspartate beta-hydroxylase C K 3 1 60391782 ASPM_BOVIN Abnormal spindle-like microcephaly-associated protein homolog C K 1 4 114617 AT5F1_BOVIN ATP synthase B chain, mitochondrial C K 1 3 124013 ATIF1_BOVIN ATPase inhibitor, mitochondrial precursor CO K 3 3 114686 ATP5H_BOVIN ATP synthase D chain, mitochondrial CO K 12 1 231615 ATP5I_BOVIN ATP synthase e chain, mitochondrial CO K 2 3 114687 ATP5J_BOVIN ATP synthase coupling factor 6, mitochondrial precursor C K 1 3 19919710 ATPase 6 C K 2 3 27807317 ATPase, aminophospholipid transporter (APLT), Class I, type 8A, member C K 1 3 61553266 ATPase, H+ transporting, lysosomal 31kD, V1 subunit E isoform 1 C K 3 2,1 14250784 ATPase, H+ transporting, lysosomal, V0 subunit D isoform 1 C K 2 3 114543 ATPB_BOVIN ATP synthase beta chain, mitochondrial precursor CO K 116 3 67010049 ATP-binding cassette sub-family A member 1 CO K 2 3 114579 ATPD_BOVIN ATP synthase delta chain, mitochondrial precursor C K 1 4 62751919 ATP-dependent Lon protease C K 2 4 22297308 Attractin C K 1 1 56967055 B Chain B, Crystal Structure Of Bosus Mitochondrial Elongation Factor O K 1 3 1083059 B54717 palmitoyl-protein thioesterase precursor C K 5 4 162731 Bactenecin 5 precursor C K 1 3 5442448 Band 3 protein C K 3 4 913808 Band 6 polypeptide B6P C K 1 3 33327729 Barrier-to-autointegration factor C K 7 2,1 2309001 BCNT C K 7 2 22779307 Bcnt C K 1 2 6224858 Beta actin CO K 30 3 312732 Beta prime cop C K 1 3 50844501 Beta tubulin CO K 42 3 61553937 Beta-1,3-N-acetylglucosaminyltransferase bGnT-6 O K 1 3 62177160 Beta-3-glucuronyltransferase P C K 1 4 62177158 Beta-3-glucuronyltransferase-S C K 1 3 25808793 Beta-actin binding proteinBetaCAP73 CO K 2 1 47523792 Beta-catenin C K 2 4 55416130 Beta-defensin 402 C K 2 4 2388780 Beta-nerve growth factor O K 1 3 7547262 Biglycan core protein precursor O K 1 3 685045 Bone sialoprotein; BSP C K 1 2 5705922 Bovine corneal protein 54; BCP 54 C K 1 3 3057087 Bovine submaxillary mucin 1 C K 2 2 285664 Bovine T cell receptor gamma chain C K 1 1 7715053 Bradykinin receptor B2 C K 1 4 1438984 Brain-type ribonuclease CO K 3 3 59858011 Branched chain aminotransferase 2, mitochondrial C K 1 1 13195201 BRCA1 C K 2 2,4 29640734 Breast and ovarian cancer susceptibility protein O K 1 3 1710369 BSP30 OK 1 3 27807233 Butyryl Coenzyme A synthetase 1 C K 1 4 30523021 Bv8/prokineticin 2-like protein CO K 2 3 46405153 C19orf10-like C K 1 4 453109 Ca(2+)-sensing receptor C K 1 3 27807135 Calcium channel, voltage-dependent, L type, alpha 1B subunit C K 1 4 2315159 Caldesmon OK 2 3 32766614 Calm2-prov protein C K 4 4 27806049 Calmodulin-dependent phosphodiesterase type 1 C K 1 4 27806721 Calpastatin C K 1 1 287744 CaMP-gated channel CO K 2 3 38322703 Capping protein (actin filament) muscle Z-line, alpha 2 C K 2 4 1838956 Capping protein, beta3 isoform C K 6 4 28192437 Carbonic anhydrase-related XI protein C K 1 1 59676559 CaSP8 and FADD-like apoptosis regulator C K 1 2 13924448 Catecholamine binding protein C K 1 3 1707415 Cathelicidin C K 1 3 809479 Cathepsin B C K 5 4 12697815 Cathepsin D CO K 4 2 59858409 Cathepsin L2 preproprotein C K 4 4 12803555 CBX1 protein C K 1 4 4928775 Cctq C K 5 2,4 56682913 CD2 antigen C K 1 4 27806911 CD9 antigen (p24) C K 1 3 19170724 CD97 protein OK 1 2 1345706 CDC2_BOVIN Cell division control protein 2 homolog (p34 protein kinase) O K 1 2 59857651 CDC37 homolog C K 1 4 61553589 Cdc42 guanine exchange factor 9 O K 1 3 59857983 CDC45-like C K 1 3 30794346 Cellular retinoic acid binding protein 1 C K 1 4 56182498 Cellular retinoic acid binding protein 2 C K 2 4 33323485 Cellular retinol binding protein 1 C K 5 4 32481980 C-Fos C K 1 1 37780029 CGI-105 protein C K 4 3 61554294 CGI-107 protein C K 1 4 59858441 CGI-30 protein OK 1 3 204 CGMP-gated channel C K 1 1 61553710 chaperonin containing TCP1, subunit 3 (gamma) C K 4 1 58760440 Chemokine (C-X-C motif) ligand 13-like C K 2 2 33415854 Chemokine receptor 7 C K 1 4 51316067 CHIA_BOVIN Acidic mammalian