Version: 2 Revision Date: 2016-01-08 Product Data Sheet Purified anti-NUP153 Catalog # / Size: 906201 / 100 µl Previously: Covance Catalog# MMS-102P Clone: QE5 Isotype: Mouse IgG1 Immunogen: The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins. Reactivity: Eukaryote Preparation: The antibody was purified by affinity chromatography. Formulation: Phosphate-buffered solution + 0.03% thimerosal. Concentration: 1 mg/ml Storage: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your Methanol fixed HeLa stained with the CoA to find the most accurate storage condition for this antibody. antibody QE5. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with the Applications: antibody to Giantin. Applications: ICC, WB, IF, IP IEM - Reported in literature Recommended Usage: Each lot of this antibody is quality control tested by Immunocytochemistry. The optimal working dilution should be determined for each specific assay condition. • WB: 1:500* • IF: 1:250 • IP: 1:50 Application Notes: This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). *Predicted MW = 250 kD This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern. Application References: 1. Pare GC, Easlick JL, Mislow JM, McNally EM, Kapiloff MS. Nesprin-1alpha contributes to the targeting of mAKAP to the cardiac myocyte nuclear envelope. Exp Cell Res 303(2):388-99, 2005. 2. N Pante, R Bastos, I McMorrow, B Burke, U Aebi. Interactions and three dimensional localization of a group of nuclear pore complex proteins. J Cell Biol 126:603-617, 1994. 3. Smythe C, et al. 2000. EMBO J. 19:3918. (WB) 4. Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed 5. Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed 6. Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed 7. Chen CY, et al. 2012. Cell. 149:565. (IF, WB) 8. Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM) 9. Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP) Description: Nucleoporin 153 (Nup153) is a component of the nuclear pore complex, and is required for the anchoring of the nuclear pore complex to the nuclear pore membrane. Nup153 has a critical role in the nuclear export of proteins and RNA, and is involved in the retention of unspliced mRNAs in the nucleus. Other Names: HNUP153, N153, Nuclear Pore Complex Protein NUP153, Nucleoporin Nup153, 153 kD nucleoporin, nuclear pore complex protein hnup153 Related Products:Product Clone Application Anti-Alpha Smooth Muscle Actin 1A4 IHC, ICC, WB Anti-Human Flightless-I Protein 116.40 IHC, WB Anti-Myosin Heavy Chain H11 WB, IF, IP Purified anti-Flightless-I Protein 116.40 IHC, WB Purified Non-muscle Myosin Heavy Chain II-B Poly19099 WB, IF, IHC For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587 Purified anti-NMHC II-C Poly19192 WB, IF For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587.