PulseNet: 20 years of making food safer to eat CENTRAL PILLAR OF US FOOD SAFETY SYSTEM PulseNet PulseNet is a national network of laboratories that solves outbreaks of foodborne by the numbers: disease. PulseNet scientists detect outbreaks—large and small—by connecting the dots between cases of similar illness that are happening anywhere in the country. 1 billion PulseNet investigations have brought about lasting changes in food industry Pounds of contaminated food recalled practices and new regulations that have made our food safer. since PulseNet was launched $507 million HOW Saved each year (medical costs and lost PulseNet works productivity) with quick outbreak detection 1 million DNA fingerprinting DNA fingerprints of foodborne bacteria 1 technology identifies the in the PulseNet USA database bacteria making people sick 89,000 Database managers check the Record number of DNA fingerprints of 2 national databases for more-than- bacteria submitted to PulseNet in 2015 expected numbers of matching DNA fingerprints, or clusters, from anywhere in the US 1,500 Clusters of illness from Salmonella, E. coli, Alert state and local health and Listeria infections annually identified 3 departments about a by PulseNet member labs possible outbreak 280 PulseNet scientists work with Multistate clusters of illness caused 4 FDA and USDA/FSIS* officials, by Salmonella, E. coli, and Listeria infections epidemiologists, and environmental identified by PulseNet each year health specialists to identify the contaminated food 83 Federal, regional, state, and local 5 Public alerted of the outbreak laboratories in the PulseNet network 40 Manufacturer may voluntarily Clusters of human illnesses tracked weekly 6 recall the contaminated food *FDA (US Food and Drug Administration) USDA/FSIS (US Department of Agriculture/Food Safety and Inspection Service) “APHL is proud of its long-standing connection The story of PulseNet to PulseNet, from the first In the beginning... software purchases to there were contaminated hamburgers whole genome sequencing.” Scott J. Becker, Executive Director, Association of Public Health Laboratories In 1993, more than 700 people got sick and 4 died in the western United States after eating at a large fast-food chain. It took public health investigators 39 days to determine that these illnesses were connected and that it was an outbreak. And it also took more A Tale of Two Outbreaks than a month after the first person fell ill to identify the culprit: hamburgers contaminated with a deadly type of E. coli bacteria. This outbreak spurred foodborne disease experts at CDC to find a way to speed up detection of foodborne outbreaks. BEFORE PulseNet A few years later, in partnership with the Association of Public 1993 Outbreak of E. coli in western states Health Laboratories (APHL), federal partners, and public health labs in 4 states, CDC created PulseNet. The goal was to days to detect outbreak: create databases that would be the central repository for DNA 39 fingerprints extracted from gut bacteria of patients with foodborne disease. Public health laboratories around the United States 726 ill, 4 deaths could submit these DNA fingerprints to PulseNet, which would be managed by CDC. When investigators searched the PulseNet databases and discovered that several ill people had bacteria with the same DNA fingerprint, they would suspect an outbreak. AFTER PulseNet Now, every state has at least one public health laboratory that 2002 Outbreak of E. coli in Colorado uses PulseNet’s technology and can access its databases of close to 1 million fingerprints of bacteria. Because it has connected days to detect outbreak: the dots between current illnesses and stored DNA fingerprints, 18 PulseNet has revolutionized the way foodborne outbreaks are detected and investigated. 44 ill, 0 deaths PulseNet’s 20-year history of making food safer to eat Outbreak of a deadly form of CDC, the Association of Public Traces a Salmonella infections in western states: Health Laboratories, federal PulseNet wins 2nd Innovations E. coli multistate outbreak to WGS used for routine > 700 illnesses; 4 children died. partners, and public health in American Government Award peanut butter/peanut surveillance of Listeria, Highlights the need for a network labs in 4 states launch a (also won in 1999) for excellence Begins using WGS on products; 700 illnesses, Campylobacter, and E. coli to identify DNA fingerprints of new foodborne surveillance and creativity in the public sector 9 deaths in 46 states, illnesses caused by at CDC and in states with foodborne bacteria network called PulseNet > 3,000 products recalled Listeria infection genetic sequencing capacity 2001 2006 2010 2014 1993 1996 2002 2009 2013 2016 1st time whole-genome PulseNet goes nationwide; Identifies spinach as sequencing (WGS) used “PulseNet and Beyond” project all 50 state public health labs source of E. coli outbreak; in a foodborne disease consolidates identification of perform DNA fingerprinting 225 sickened and 5 investigation. PulseNet uses foodborne bacteria into a single, of foodborne bacteria deaths in 27 states; WGS on samples from a fast, and efficient process under prompts nationwide recall cholera outbreak in Haiti Advanced Molecular Detection (AMD) PulseNet has made these foods safer to eat: Peanut butter, Sprouts, Eggs, Tree nuts, Poultry products, Leafy greens, Case study: Tomatoes, Frozen entrees, Lunch meat, Spices, Melons Listeria-contaminated caramel apples In November 2014, PulseNet scientists PulseNet in action: Making food safer detected two clusters of illness (35 people from 12 states, including 7 who Food safety requires that we pay close attention infection to raw almonds grown in California. died) caused by the foodborne bacteria to every step in the food supply chain, from farm The outbreak prompted regulators to mandate Listeria. Using traditional lab analysis to fork. Diligent efforts by farmers, producers, that almonds for sale in the United States methods, the scientists linked sick patients who were likely to have eaten distributors, regulators, and consumers have be pasteurized (by fumigation or hot steam the same contaminated food. They also made the US food supply among the safest treatment) to control the problem and prevent used whole-genome sequencing (WGS) in the world. Nevertheless, an estimated 48 similar outbreaks in the future. to more precisely determine which million people, or 1 in 6 Americans, get sick illnesses were linked. Armed with this Outbreak investigations supported by information, investigators interviewed every year from foodborne illnesses. That is PulseNet help scientists find gaps in food patients about foods they had eaten where PulseNet comes in. safety systems that would otherwise be and identified a new source of Listeria infection: caramel apples. During outbreak investigations, scientists can unrecognized. Information from PulseNet has use PulseNet data to identify the specific type stimulated production changes by the food Real-time WGS helped investigators of bacteria that caused people to get sick, industry and helped federal agencies create solve the outbreak at least 1 week track down the food that was contaminated, new or improved recommendations and sooner than would have been possible using traditional methods. The apple and find out what happened to cause the regulations to make our food supply safer. producer and several caramel apple contamination. For example, in the early 2000s, manufacturers recalled apples to PulseNet traced an outbreak of Salmonella prevent more illnesses. PulseNet’s 20-year history of making food safer to eat Outbreak of a deadly form of CDC, the Association of Public Traces a Salmonella infections in western states: Health Laboratories, federal PulseNet wins 2nd Innovations E. coli multistate outbreak to WGS used for routine > 700 illnesses; 4 children died. partners, and public health in American Government Award peanut butter/peanut surveillance of Listeria, Highlights the need for a network labs in 4 states launch a (also won in 1999) for excellence Begins using WGS on products; 700 illnesses, Campylobacter, and E. coli to identify DNA fingerprints of new foodborne surveillance and creativity in the public sector 9 deaths in 46 states, illnesses caused by at CDC and in states with foodborne bacteria network called PulseNet > 3,000 products recalled Listeria infection genetic sequencing capacity 2001 2006 2010 2014 1993 1996 2002 2009 2013 2016 1st time whole-genome PulseNet goes nationwide; Identifies spinach as sequencing (WGS) used “PulseNet and Beyond” project all 50 state public health labs source of E. coli outbreak; in a foodborne disease consolidates identification of perform DNA fingerprinting 225 sickened and 5 investigation. PulseNet uses foodborne bacteria into a single, of foodborne bacteria deaths in 27 states; WGS on samples from a fast, and efficient process under prompts nationwide recall cholera outbreak in Haiti Advanced Molecular Detection (AMD) The Future of PulseNet: Faster, cheaper, and better foodborne disease detection A new test Culture-independent diagnostic tests, or CIDTs, are new tests that can detect the DNA of bacteria directly from patient samples like stool. As Transforming disease detection their name implies, CIDTs do not need a culture (cells grown in a lab) to In 2013, CDC began using whole-genome sequencing (WGS) identify the bacteria that caused a patient’s illness. Clinical laboratories to detect outbreaks caused by Listeria, the most deadly are increasing their