NELFA Antibody Cat. No.: XW-7655 NELFA Antibody Specifications HOST SPECIES: Chicken SPECIES REACTIVITY: Human, Mouse, Rat IMMUNOGEN: 334-528 TESTED APPLICATIONS: WB NELFA antibody can be used for the detection of WHSC2 (C-195) by Western blot, may also APPLICATIONS: work for IHC and ICC. PREDICTED MOLECULAR 57.3 kDa (calculated) WEIGHT: Properties PURIFICATION: Antigen affinity-purified CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Phosphate-Buffered Saline. No preservatives added. CONCENTRATION: 1 mg/mL September 24, 2021 1 https://www.prosci-inc.com/nelfa-antibody-7655.html NELFA antibody can be stored at 4˚C for short term (weeks). Long term storage should be STORAGE CONDITIONS: at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: NELFA WHSC2, P/OKcl.15, Negative elongation factor A, Wolf-Hirschhorn syndrome candidate 2 ALTERNATE NAMES: protein, NELF-A, WHSC2, WHSC2 (C195) ACCESSION NO.: NP_005654.1 PROTEIN GI NO.: 5032227 GENE ID: 7469 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Wolf-Hirschhorn syndrome candidate 2 protein; WHSC2 protein; NELFA. WHSC2 is expressed ubiquitously with higher levels in fetal than in adult tissues. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. BACKGROUND: The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor- specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. 1) Narita,T., Yamaguchi,Y., Yano,K., Sugimoto,S., Chanarat,S., Wada,T., Kim,D. K., Hasegawa,J., Omori,M., Inukai,N., Endoh,M., Yamada,T. and Handa,H. Human transcription REFERENCES: elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex. Mol. Cell. Biol. 23 (6), 1863-1873 (2003). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 2 https://www.prosci-inc.com/nelfa-antibody-7655.html.