OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AM09389PU-S Monoclonal Antibody to AKR1C1 / DHH1 (1-323) - Purified Alternate names: 2-dihydrobenzene-1, 2-diol dehydrogenase, 20-alpha-hydroxysteroid dehydrogenase, Aldo-keto reductase family 1 member C1, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1/2, High-affinity hepatic bile acid-binding protein, Trans-1 Quantity: 50 µl Concentration: 1.0 mg/ml Background: The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta- andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively. Uniprot ID: Q04828 NCBI: NP_001344 GeneID: 1645 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), AM03095PU-N Controls: Clone: AT6D10 Immunogen: Recombinant Human AKR1C1 (1-323aa) purified from E. coli Format: State: Liquid purified Ig fraction Purification: Affinity Chromatography on Protein G Buffer System: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol Applications: ELISA. Western blot: 1/1000. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Immunocytochemistry / Immunoflourescence. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: The antibody recognizes Human AKR1C1 at aa 1-323. Species Reactivity: Tested: Human Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. L.C. Giudice, et al. (2004) Lancet. 3644: 1789–1799 2. P.G. Hompes, et al. (2007) Gynecol. Endocrinol. 23: 5–12. 3. Berkley KJ, Rapkin AJ, Papka RE. The pains of endometriosis. Science. 2005 Jun For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. AV/20160105 1 / 3 AM09389PU-S: Monoclonal Antibody to AKR1C1 / DHH1 (1-323) - Purified 10;308(5728):1587-9. PubMed PMID: 15947176. Pictures: Western blot analysis: The extracts of HepG2 (33 ug) were resolved by SDS- PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ICC/IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human AKR1C1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. AV/20160105 2 / 3 AM09389PU-S: Monoclonal Antibody to AKR1C1 / DHH1 (1-323) - Purified Flow cytometry analysis of AKR1C1 in A431 cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antib ody was mouse IgG (black line). Western blot analysis: The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS - PAGE, transferred to PVDF membran e and probed with anti human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AKR1C1 Lane 2 . : HepG2 c ell lysate Lane 3 . : Raji cell lysate For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. AV/20160105 3 / 3 Powered by TCPDF (www.tcpdf.org).