Catalog # Aliquot Size P29-911-05 3 x 5 nmol P29-911-20 3 x 20 nmol P29-911-50 3 x 50 nmol PTPN2 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P29-911 Lot # Z2085-24 Specificity Formulation PTPN2 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPN2 expression. shipped at room temperature. Product Description Reconstitution Protocol PTPN2 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPN2 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_080422. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot TC-PTP, PTPT, TCELLPTP, TCPTP 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PTPN1 (PTP1B), Active P28-20G below -20oC. After resuspension, siRNA stock solutions ≥2 PTPN11 (SHP2), Active P38-21G µM can undergo up to 50 freeze-thaw cycles without PTPN12 (PTP-PEST), Active P39-21G significant degradation. For long-term storage, it is PTPN13 (FAP-1), Active P40-21G recommended that the siRNA is stored at -70oC. For most PTPN2 (TC-PTP), Active P29-20G favorable performance, avoid repeated handling and PTPN6 (SHP1), Active P33-20G multiple freeze/thaw cycles. PTPN7 (LC-PTP), Active P34-20G Anti-PTPN11 (SHP2) P38-63CR Scientific Background Anti-PTPN11 (SHP2) P38-63BR Protein tyrosine phosphatase, non-receptor type 2 (PTPN2), is one of the most abundant mammalian tyrosine phosphatase. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family PTPN2 siRNA Set I siRNA duplexes targeted against three exon regions (1). By virtue of protein tyrosine phosphatase activity, PTPN2 is known to be a signaling molecule that regulate Catalog # P29-911 a variety of cellular processes including cell growth, Lot # Z2085-24 differentiation, mitotic cycle, and oncogenic Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol transformation involved in cell communication and signal Format Lyophilized powder transduction (2). Stability 1yr at -70oC from date of shipment Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is References recommended that the lyophilized 1. Gupta, S. et al: A nuclear protein tyrosine and resuspended siRNAs are stored at phosphatase activates p53 and induces caspase-1- or below -20oC. After resuspension, dependent apoptosis. FEBS Lett. 2002; 532(1-2):61-6. siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles 2. Yamamoto, T. et al: The nuclear isoform of protein- without significant degradation. For tyrosine phosphatase TC-PTP regulates interleukin-6- long-term storage, it is recommended mediated signaling pathway through STAT3 that the siRNA is stored at -70oC. For most favorable performance, avoid dephosphorylation. Biochem. Biophys. Res. repeated handling and multiple Commun. freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .