G-Biosciences Molecular Biology Handbook & Selection Guide • Protein Estimation Assays • Lysis Buffers & Systems • Apoptosis Assays • Protein Fractionation Kits • Cytotoxicity Assays • Dialysis (Micro) System • SAM Methyltransferase Assays • Electrophoresis Clean-Up • Protease Assays • Concentration Systems • Phosphatase Assays • Contamination Removal • Peroxide Assay • Protease Inhibitor Cocktails • Proteomic Grade Detergents • Individual Protease Inhibitors • Research Grade Detergents • Protease Assays • Non-Ionic, Ionic & Zwitterionic • Proteases for Mass Spec. • Detergent Estimations • Sequencing Grade Proteases • Detergent Removal Systems • 1-Hour Western System • Gel Preparation Chemicals • Transfer Buffers & Membranes • Protein Marker Ladders • Membrane Stains • Electrophoresis Buffers • Blocking Buffers • Reducing & Alkylating Reagents • Secondary Antibodies • Protein Gel Stains • Detection Reagents • Reprobing Reagents • Protein Sample Preparation • Affinity Resins • Protein Clean-Up Systems • 6X His Protein Purification Kits • Electrophoresis Reagents • GST Protein Purification Kits • Mass Spec Grade Protease • Antibody Purification • InGel Digestion Kits • Activated Resins • Peptide Generation Reagents • Buffers & Reagents • Biotin Labeling • Carrier Proteins • Cell Surface Protein Labeling • Peptide Coupling Systems • Agarose Coupling Kits • Antibody Purification Resins • Fluorescent Dye Labeling Kits • Antibody Fragmentation Kits • Enzyme Labeling Systems • Coated Plates • Blocking Buffers • Homobifunctional • Wash Buffers • Heterobifunctional • Secondary Antibodies • Optimizer Systems • Detection Reagents • Cross-Linking Systems • Antibody Labeling Systems • Apoptosis Assays • DNA Isolation • Cytotoxicity Assays • Transformation & Screening • SAM Methyltransferase Assays • Polymerase Chain Reaction • Protease Assays • Agarose Electrophoresis • Phosphatase Assays • RNA Isolation • Peroxide Assay • Yeast Transformation • ELISA Table Of Contents Genomic DNA Purification 2 PCR Clean Up ........................................................................15 Spin-OUT™ for PCR...................................................................15 Introduction ...........................................................................2 Reverse Transcriptases (RT) .................................................15 Purest DNA with OmniPrep™ .................................................2 ™ M-MLV Reverse Transcriptase ................................................15 OmniPrep ...............................................................................2 AMV Reverse Transcriptase....................................................15 OmniPrep™ for Tissue .............................................................2 OmniPrep™ for Blood ...............................................................3 RT-PCR Kits ............................................................................16 OmniPrep™ for Fungi ...............................................................3 First-strand cDNA Synthesis Kit .............................................16 OmniPrep™ for Gram Positive Bacteria ..................................3 1-Step RT-PCR Kit ...................................................................16 ™ 2-Step RT-PCR Kit ...................................................................16 OmniPrep for Yeast ...............................................................3 ™ OmniPrep™ for Plant ................................................................4 Tri-Xtract .................................................................................16 OmniPrep™ for Mouse Tail ......................................................4 RNA Purification 17 ™ Spin Format Isolation with GET ..........................................4 Arrest™ Extraction Buffer ........................................................17 ™ GET DNA Template ................................................................4 GET™ Total RNA .......................................................................17 ™ GET Plant DNA Template ......................................................4 TOTAL Arrest™ .........................................................................17 Protein Free DNA with XIT™ ...................................................5 NUCLEIC dotMETRIC™ Assay ..................................................18 XIT™ DNA from Tissue .............................................................5 RNase-DETECT™ .....................................................................18 XIT™ DNA from Cells ................................................................5 RNaseOUT™ ............................................................................18 XIT™ DNA from Blood ...............................................................5 RNasin ....................................................................................18 XIT™ DNA from Buccal Cells ....................................................6 XIT™ DNA from FFPE Tissue ....................................................6 Nucleic Acid Electrophoresis 19 XIT™ DNA from Mouse Tail ......................................................6 Geno-ElectroPhore™ ................................................................19 XIT™ DNA from Plant Tissues ..................................................6 Power Supplies ......................................................................19 XIT™ DNA from Gram Positive Bacteria ..................................7 GT 250 Power Pack™...............................................................19 XIT™ DNA from Gram Negative Bacteria ................................7 E-Power™ 150 Power Supply...................................................19 XIT™ DNA from Yeast ...............................................................7 E-Power™ 100 Power Supply ...................................................19 XIT™ Mitochondrial DNA ..........................................................7 G-CAPSULE™ ............................................................................20 PCR Template DNA ................................................................8 GET™ AGAROSE DNA ...............................................................20 OmniTemplate™ .......................................................................8 geneEXIT™ ................................................................................20 Rapid DNA Template Prep™ ....................................................8 Glow™ Dyes .............................................................................21 Yeast Geno-DNA-Template™ ....................................................8 DNA Ladders..........................................................................22 Plant Geno-DNA-Template™ ....................................................9 DNAmark™ Logic DNA Ladder ................................................22 High Molecular Weight DNA .................................................9 DNAmark™ 100bp Plus Ladder ..............................................22 MegaLong™ ..............................................................................9 DNAmark™ 100bp Ladder ......................................................22 Plasmid DNA Tools 10 Accessories, Buffers & Chemicals .......................................23 Electrophoresis Running Buffers ...........................................23 Plasmid Isolation ...................................................................10 Agarose Powders ....................................................................23 GET™ Plasmid DNA Miniprep ..................................................10 LabSafe™ Nucleic Acid Stain ..................................................23 GET™ Plasmid DNA Mediprep .................................................10 FASTsilver™ ..............................................................................23 GET™ Plasmid DNA Maxiprep .................................................10 Bacteria Transformation .......................................................11 Hybridization Buffers 24 ™ Z-Competent™ E. coli Transformation ....................................11 EKONO ...................................................................................24 ™ Plasmid Screening Reagents .................................................11 HYB-LINK ...............................................................................24 Plasmid Screening ................................................................11 Other Hybridization Buffers ....................................................24 Plasmid Screening ToothPick™ ...............................................11 Yeast Research Tools 25 ToothPick™-PCR .......................................................................11 EZ Yeast™ Plasmid Prep ..........................................................25 DNA Cleanup & Concentration 12 Fast Yeast Transformation™ ....................................................25 Yeast Related Buffers .............................................................25 GET™ CLEAN DNA ....................................................................12 Yeast Geno-DNA-Template™ ....................................................26 pinkCLEANUP™ .......................................................................12 LongLife™ Zymolyase® ...........................................................26 Genomic Tube-O-DIALYZER™ ...................................................12 LongLife™ Enzyme Preparations .............................................26 Spin-OUT™