APIDOLOGIE Supplementary Materials Assessment of the pollen diet in a wood-dwelling augochlorine bee (Halictidae) using different approaches Milagros Dalmazzo and Favio Gerardo Vossler* * Corresponding author: F.G.Vossler, [email protected] Laboratorio de Actuopalinología, CICyTTP - CONICET / FCyT - UADER, Dr. Materi y España, E3105BWA, Diamante, Entre Ríos, Argentina ESM 1. Two-way clustering showing two main groups of nests (A and B) and four groups of pollen types (1 to 4). Values of the matrix indicate percentage of the pollen grains per nest. ESM 2. Comparison between the number of floral records obtained by floral visitation and pollen analysis methods for Augochlora amphitrite, A. alexanderi, A. esox and A. isthmii. References: x*: records from the present study; x: records from other studies (cited below); x°: records from both the present study and the studies cited below; x** indicates a composition of less than 5% of pollen grains; PA: pollen analysis; FV: floral visitation; ND: no data; Pan: Panamá; BC: Barro Colorado; Arg: Argentina; Bs As: Buenos Aires; SF: Santa Fe; Br: Brazil; MG: Minas Gerais; PR: Paraná; RS: Rio Grande do Sul; SC: Santa Catarina; SP: Sao Paulo. Literature references: 1: Antonini and Martins 2003; 2: Dalmazzo 2010; 3: Dalmazzo and Roig-Alsina 2011; 4: Gimenes 2002; 5: Gonçalves and Melo 2005; 6: Imperatriz-Fonseca et al. 2011; 7: Krug et al. 2010; 8: Lopes-Azambuja and Blochtein 2007; 9: Minussi and Alves dos Santos 2007; 10: Schlindwein 1998; 11: Schlindwein and Wittmann 1997; 12: Singer and Cocucci 1999; 13: Steiner et al. 2010; 14: Wcislo et al. 2003; 15: Zillikens et al. 2001; 16: Present study. ESM 3. Fresh pollen provision of Augochlora amphitrite obtained from a nest aggregation at Ribera Norte. Sawdust and one egg can also be seen. ESM2. Comparison between the number of floral records obtained by floral visitation and pollen analysis methods for Augochlora amphitrite and for the tropical and subtropical Augochlora species (A. alexanderi, A. esox and A. isthmii). References: x*: records from the present study; x: records from other studies (cited below); x°: records from both the present study and the studies cited below; x** indicates a composition of less than 5% of pollen grains; PA: pollen analysis; FV: floral visitation; ND: no data; Pan: Panamá; BC: Barro Colorado; Arg: Argentina; Bs As: Buenos Aires; SF: Santa Fe; Br: Brazil; MG: Minas Gerais; PR: Paraná; RS: Rio Grande do Sul; SC: Santa Catarina; SP: Sao Paulo. Literature references: 1: Antonini and Martins 2003; 2: Dalmazzo 2010; 3: Dalmazzo and Roig-Alsina 2011; 4: Gimenes 2002; 5: Gonçalves and Melo 2005; 6: Imperatriz-Fonseca et al. 2011; 7: Krug et al. 2010; 8: Lopes-Azambuja and Blochtein 2007; 9: Minussi and Alves dos Santos 2007; 10: Schlindwein 1998; 11: Schlindwein and Wittmann 1997; 12: Singer and Cocucci 1999; 13: Steiner et al. 2010; 14: Wcislo et al. 2003; 15: Zillikens et al. 2001; 16: Present study. Augochlora species Plant families and species / pollen types A. alexanderi A. amphitrite A. esox A. isthmii PA FV PA FV PA FV PA FV Alismataceae Echinodorus grandiflorus (Cham.& Schltdl.) x* x* Micheli Sagittaria montevidensis x* Anacardiaceae x** Schinus polygamus (Cav.) Cabrera x Amaranthaceae x** Apocynaceae x** Asclepias curassavica L. x* Oxypetalum sp. x Aquifoliaceae Ilex x** Arecaceae x Syagrus romanzoffiana x* Asteraceae x x** Aspilia leucoglossa Malme x Aspilia montevidensis (Spreng.) Kuntze x Baccharis grandimucronata I.L. Teodoro x Baccharis tridentata Vahl x Baccharis sp. x* type Baccharis x* Bidens pilosa L. x x Calea parvifolia (DC.) Baker x Carduus acanthoides L. x* Cichorium intybus L. x* Clibadium armanii (Balb.) O.E. Schulz x Cynara cardunculus L. x Erigeron maximus DC. x x Erigeron sp. x Eupatorium itatiayense Hieron. x x Gymnocoronis spilanthoides x* Holocheilus heracioides (D. Don) Cabrera x Hypochaeris brasiliensis (Less.) Hook. & Arn. x Piptocarpha oblonga (Gardner) Baker x Senecio brasiliensis (Spreng.) Less. x Senecio confusus Britten x Senecio selloi (Spreng.) DC. x Smallanthus connatus x* Sonchus oleraceus L. x* x* Sphagneticola trilobata (L.) Pruski x Tessaria integrifolia Ruiz & Pav. x* Trixis praestans (Vell.) Cabrera x Verbesina sordescens DC. x Vernonia chamissonis Less. x x Vernonia flexuosa Sims x Vernonia nudiflora Less. x Vernonia plantaginoides (Less.) Hieron. x Vernonia simplex Less. x Vernonia tweedieana Baker x x Vernonia westiniana Less. x x Vernonia sp. x x Balsaminaceae Impatiens walleriana Hook. f. x Bignoniaceae Bignonia callistegioides Cham. x Dolichandra unguis-cati (L.) L.G. Lohmann x° Jacaranda caroba (Vell.) A. DC. x Jacaranda puberula Cham. x x Memora peregrina (Miers) Sandwith x x Boraginaceae Cordia trichoclada DC. x Bromeliaceae Aechmea lindenii (E. Morren) Baker x Aechmea nudicaulis (L.) Griseb. x Vriesea vagans (L.B. Sm.) L.B. Sm. x Cactaceae Notocactus polyacanthus (Link & Otto) Theunissen x Notocactus sellowii Buxb. x Opuntia brunneogemmia (F. Ritter) Schlindwein x Opuntia viridirubra (F. Ritter) P.J. Braun & Esteves x Parodia ottonis (Lehm.) N.P. Taylor x Chloranthaceae Hedyosmum brasiliense Miq. x Clusiaceae type Clusia melchiori (as aff. C. melchiori in 14) x type Vismia macrophylla (as aff. V. macrophylla in x 14) Vismia x Commelinaceae Commelina diffusa x* Commelina erecta L. x* Tradescantia fluminensis Vell. x* Convolvulaceae x** Bonamia sp. x Convolvulus crenatifolius Ruiz & Pav. x Ipomoea alba x* Ipomoea cairica (L.) Sweet x* x° Ipomoea pes-caprae (L.) R. Br. x x Ipomoea purpurea (L.) Roth x* x Ipomoea sp. x Merremia sp. x Cucurbitaceae Cucumis sp. x Cucurbita maxima Duchesne x Cucurbita moschata (Lam.) Poir. x Cucurbita pepo L. x Cunoniaceae Weinmannia discolor Gardner x Euphorbiaceae x** type Croton x* Fabaceae Calliandra brevipes Benth. x Calliandra tweediei Benth. x Crotalaria sp. x Desmodium sp. x Inga vulpina Benth. x x Machaerium sp. x Senna corymbosa (Lam.) H.S. Irwin & Barneby x* Senna sp. x Stylosanthes sp. x Iridaceae Cypella brasiliensis (Baker) Roitman & J.A. Castillo x Herbertia pulchella Sweet x Lamiaceae x* Clerodendrum sp. x Hyptis suaveolens (L.) Poit. x Marsypianthes chamaedrys (Vahl) Kuntze x x Lythraceae Cuphea glutinosa Cham. & Schltdl. x Cuphea racemosa (L. f.) Spreng. x Heimia myrtifolia Cham. & Schltdl. x Malpighiaceae x** Malvaceae Pavonia hastata Cav. x Pavonia sepium A. St.-Hil. x° Pavonia x* Sida planicaulis Cav. x Sida rhombifolia L. x° Sphaeralcea bonariensis (Cav.) Griseb. x* Melastomataceae x Tibouchina radula Markgr. x Myrtaceae x** Myrcia rostrata DC. x Psidium littorale var. longipes (O. Berg) Fosberg x Onagraceae Ludwigia bonariensis (Micheli) H. Hara x* Ludwigia elegans (Cambess.) H. Hara x° Ludwigia grandiflora subsp. hexapetala x (Hook. & Arn.) G.L. Nesom & Kartesz Ludwigia longifolia (DC.) H. Hara x x Ludwigia octovalvis (Jacq.) P.H. Raven x Ludwigia peploides (Kunth) P.H. Raven x* Ludwigia peruviana (L.) H. Hara x x Ludwigia x* x Oenothera ravenii W. Dietr. x Orchidaceae Campylocentrum aromaticum Barb. Rodr. x Oxalidaceae Oxalis rhombeo-ovata A. St.-Hil. x Phytolaccaceae x** Phytolacca thyrsiflora J.A. Schmidt x Poaceae x** Polygonaceae Polygonum punctatum Elliott x Pontederiaceae Pontederia lanceolata Nutt. x Rosaseae Rubus rosifolius Sm. x Rubiaceae x** Borreria poaya (A. St.-Hil.) DC. x Diodia apiculata (Roem. & Schult.) Delprete x Galianthe fastigiata Griseb. x Psychotria suterella Müll. Arg. x Richardia brasiliensis Gomes x Rudgea jasminoides (Cham.) Müll. Arg. x Spermacoce schumannii Delprete x type Warscewiczia coccinea (as aff. W. coccinea in x 14) Sapindaceae x** Toulicia tomentosa Radlk. x Solanaceae x** Solanum lycocarpum A. St.-Hil. x type Solanum? x* Tiliaceae x** Tropaeolaceae Tropaeolum majus L. x Verbenaceae Lantana camara L. x Stachytarpheta cayennensis (Rich.) Vahl x Verbena litoralis Kunth x TOTAL 2 ND 15 82 6 53 14 ND Arg. (Bs As, SF); Br. Pan. Arg. Br. Br. (MG, Localities Pan. (BC) (BC) (Bs As) (PR, (SC) RS, RS, SC) SC, SP) 2;3;4;5; 6;7;8;9; 1;6;13, Literature references 14 16 15 14 10;11;1 15 2;13;16 .