RESEARCH USE ONLY DATA SHEET Rev 121411D Clinical customers please refer to IVD / ASR Data Sheet Calponin-1 (Clone EP798Y) Rabbit Monoclonal Antibody Produced by Epitomics, Inc. Using Technology Licensed Under Patent no. 5,675,063 Cat. #RM-2102-S0, or -S (0.1ml or 1.0ml Supernatant) (Purified Ab with BSA and Azide) Cat. #RM-2102-R7 (7.0ml) (Ready-to-Use for Immunohistochemistry) Cat. #RM-2102-RQ (12.0ml) (Ready-to-Use for Immunohistochemistry) Please note this data sheet has been changed effective December 14, 2011 Description: Calponin, a thin filament associated protein Storage and Stability: is implicated in the regulation and modulation of smooth Store vial at 40C. When stored at 2-80C, this antibody is muscle contraction. It is capable of binding to actin, stable for 24 months. calmodulin, troponin C and tropomyosin. Calponin is expressed in smooth muscle and tissues containing Suggested References: significant amounts of smooth muscle. Two isoforms of 1. Douglas-Jones A et al. Histopathology 2005;47:202 calponin exist whose molecular weights are 34kDa and 2. Rabban J T et al. Mod Path 2006;19:1351 29kDa. Expression of the 29kDa form is primarily restricted 3. Werling R W et al. AJSP 2003;27:82, to muscle of the urogenital tract. The expression of 4. Lazard D et al. Proc Natl Acad Sci USA 1993;90:999 calponin has also been demonstrated in myoepithelial cells 5. Zhang R R et al. Breast Cancer Res 2003;5:R151-6 from benign and malignant breast lesions. It stains smooth muscle, myoepithelial cells, myofibroblasts, keratinocytes Limitations and Warranty: and nerve fibers. It identifies myoepithelial cells in breast Our products are intended FOR RESEARCH USE ONLY lesions, and helps differentiate breast collagenous and are not approved for clinical diagnosis, drug use or spherulosis (positive) from adenoid cystic carcinoma. therapeutic procedures. No products are to be construed Adenoid cystic carcinoma in salivary gland tumors is as a recommendation for use in violation of any patents. calponin positive. We make no representations, warranties or assurances as to the accuracy or completeness of information provided Mol. Wt. of Antigen: 29/ 34kDa on our data sheets and website. Our warranty is limited to the actual price paid for the product. Lab Vision is not liable Clone: EP798Y for any property damage, personal injury, time or effort or economic loss caused by our products. Species Reactivity: Human, Others-not known. Material Safety Data: A synthetic peptide derived from near the C- Immunogen: This product is not licensed or approved for administration ter. of human Calponin-1 to humans or to animals other than the experimental Applications and Suggested Dilutions: animals. Standard Laboratory Practices should be followed • Immunohistology (Formalin/paraffin) when handling this material. The chemical, physical, and Use Ab 1:100 for 20 min at RT using the LP Detection toxicological properties of this material have not been System) thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and Use Ab 1:200 for 20 min at RT using the Quanto Detection ingestion. The material contains 0.09% sodium azide as a System) preservative. Although the quantity of azide is very small, * (Staining of formalin-fixed sections require heat appropriate care should be taken when handling this induced antigen retrieval in citrate, pH 6.0, heating to material as indicated above. The National Institute of 98°C for 20 min using the Thermo Scientific Occupational Safety and Health has issued a bulletin citing PTModule.) the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the The optimal dilution for a specific application should be plumbing systems. Sodium azide forms hydrazoic acid in determined by the investigator. acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal Positive Control: Breast Carcinoma drainage pipes. Cellular Localization: Cytoplasm Myoepithelial cells Supplied As: Tissue culture supernatant with 15mM sodium azide. or Prediluted antibody which is ready-to-use for immunohistochemistry. Lab Vision Corporation Thermo Fisher Scientific 46360 Fremont Blvd. Anatomical Pathology Fremont, CA 94538-6406, USA Tudor Road, Manor Park US Toll Free: 1 (800) 522-7270 Runcorn, Cheshire WA7 1TA, UK Phone: +1 (269) 544-5600 Tel: +44 (0) 1928 534 050 Fax: 1 (269) 372-2674 Fax: +44 (0) 1928 534 049 www.thermoscientific.com/labvision [email protected] RESEARCH USE ONLY DATA SHEET Rev 121411D Clinical customers please refer to IVD / ASR Data Sheet Calponin-1 (Clone EP798Y) Rabbit Monoclonal Antibody Produced by Epitomics, Inc. Using Technology Licensed Under Patent no. 5,675,063 Cat. #RM-2102-S0, or -S (0.1ml or 1.0ml Supernatant) (Purified Ab with BSA and Azide) Cat. #RM-2102-R7 (7.0ml) (Ready-to-Use for Immunohistochemistry) Cat. #RM-2102-RQ (12.0ml) (Ready-to-Use for Immunohistochemistry) Please note this data sheet has been changed effective December 14, 2011 Formalin-fixed, paraffin-embedded human breast carcinoma stained with Calponin-1 (Cat. #RM-2102-S) using peroxidase- conjugate and DAB chromogen. Note membrane staining. For Research Use Only Lab Vision Corporation Thermo Fisher Scientific 46360 Fremont Blvd. Anatomical Pathology Fremont, CA 94538-6406, USA Tudor Road, Manor Park US Toll Free: 1 (800) 522-7270 Runcorn, Cheshire WA7 1TA, UK Phone: +1 (269) 544-5600 Tel: +44 (0) 1928 534 050 Fax: 1 (269) 372-2674 Fax: +44 (0) 1928 534 049 www.thermoscientific.com/labvision [email protected] .