Leibniz Institute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH PLANT VIRUS DIAGNOSTICS CATALOGUE 2021 Images on front cover center: leaf curl symptoms from tomato (cv. Harzfeuer) infec�on with Tomato yellow leaf curl virus PV-0560 (le�) and necro�c leaf curl symptoms on tomato (cv. Harzfeuer) infected with Tomato leaf curl New Delhi virus PV-1285 (middle/ right) bo�om le�: Turnip yellows virus infected oilseed rape leaves displaying interveinal yellowing and purpling of leaf margins bo�om right: typical vein clearing caused by Malva vein clearing virus in hollyhock (Alcea rosea) Images on back cover top le�: Potato virus Y induced strong vein necrosis on a potato leaf top right: necro�c streak symptoms of ‘black death’ on Helleborus flowers and leaves associated with Helleborus net necrosis virus ELISA Product Informa�on The high quality diagnos�c products and services offered by the Plant Virus Department of Leibniz-Ins�tute DSMZ are based on more than 30 years of experience. Together with our con�nually improved quality management system, this guarantees our customers the highest level of sa�sfac�on. Enzyme-linked immunosorbent assay (ELISA) is s�ll the most widely used method for large scale tes�ng of plant viruses. DSMZ offers about 250 high quality serological reagents and posi�ve controls for rou�ne ELISA detec�on of the most important viruses infec�ng agricultural and hor�cultural crops. The available diagnos�c products are listed in alphabe�c order in this catalogue. Please use our online catalogue to check the availability of our wide range of infec�ous virus isolates which are not listed in this diagnos�c catalogue. ELISA sets ● contain IgG and conjugate (IgG-AP) for DAS-ELISA ● contain IgG, Mab and RAM-AP for TAS-ELISA ● are op�mized and provided for a test volume of 200µl and are supplied as 100 test units (smallest unit) up to >1000 test units. ELISA controls Plant virus materials for use as posi�ve/nega�ve controls in ELISA consist of freeze-dried homogenates of virus infected or healthy plants and are to be recons�tuted in buffer prior to use. In some cases, desiccated leaf material may be provided as ELISA controls. ● Dissolve controls consis�ng of freeze dried plant homogenate in 2ml sample extrac�on buffer. ● Controls consis�ng of freeze dried leaf material have to be homogenized in sample extrac�on buffer (1:50 weight/volume) prior to use. Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Our Next Genera�on Sequencing services to support plant health Next genera�on sequencing (NGS) technologies offer unprecedented opportuni�es to reveal the phytosanitary status of a plant sample, without a priori knowledge of a par�cular organism. The genera�on of high amounts of sequencing data allows a global profiling of samples on a large scale, with rapid results and atlow costs. We provide NGS services for analysis of plant samples on the basis of our profound laboratory competences covering all steps of a workflow - from DNA/RNA extrac�on, state of the art library prepara�on, sequencing on Illumina pla�orms and comprehensive bioinforma�cs analyses. We offer support at every step including consulta�on on project planning, real-�me updates onthe process, fast delivery of results and post-analysis evalua�on to support management decisions. Our services enable: • global analysis of the phytosanitary status of plants • rapid and precise iden�fica�on of pathogens • discovery of novel or rare variants • characteriza�on of the gene�c varia�on and popula�on composi�on • the safe movement and interna�onal exchange of nursery materials and elite propaga�on and breeding lines For any further informa�on about our services, please contact us at [email protected] or directly contact our NGS and bioinforma�cs specialist Dr. Paolo Margaria at [email protected]. Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Pathogen Test Format Product Code Virus species for which ELISA an�sera sets and the respec�ve controls are available are listed below in alphabe�c order. An�sera and controls always need to be ordered separately. Our webshop offers a convenient way to place your orders and helps to process your orders asfast as possible: h�ps://www.dsmz.de/catalogues/catalogue-plant-viruses-and-an�sera.html. Please use our online catalogue also to check the availability of our wide range of infec�ous virus isolates which are not listed in this diagnos�c catalogue. African cassava mosaic virus (ACMV) see cassava mosaic geminiviruses Agropyron mosaic virus (AgMV, Rymovirus) DAS-ELISA RT-0729 Posi�ve control PC-0729 Nega�ve control NC-0729 Alfalfa mosaic virus (AMV, Alfamovirus) DAS-ELISA RT-0779 Posi�ve control PC-0779 Nega�ve control NC-0779 Alstroemeria mosaic virus (AlMV, Potyvirus) DAS-ELISA RT-0121 Posi�ve control PC-0328 Nega�ve control NC-0328 Alternanthera mosaic virus (AltMV, Potexvirus) DAS-ELISA RT-0725 Posi�ve control PC-0725 Nega�ve control NC-0725 Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Pathogen Test Format Product Code Andean potato latent virus (APLV, Tymovirus) DAS-ELISA RT-0978 Detec�on of APLV-Col/Col-2 isolates and Posi�ve control PC-0060 Andean potato mild mosaic virus (APMMV) Nega�ve control NC-0061 (formerly recognized as APLV-Hu isolates). Cross reac�on with Eggplant mosaic virus (EMV) Posi�ve control PC-1149 Note: In accordance with Council Regula�on (EC) No 428/2009 (and following amendments), APLV/APMMV isolates and posi�ve controls will not be shipped outside EU countries. Please use Eggplant mosaic virus (EMV) posi�ve control PC-1149 instead. Andean potato mild mosaic virus (APMMV, Tymovirus) see Andean potato latent virus (APLV) Andean potato mo�le virus (APMoV, Comovirus) DAS-ELISA RT-0005 Detec�on of ApMoV strains B, C, H. Posi�ve control PC-0057 Nega�ve control NC-0057 Angelonia flower break virus (AFBV, Carmovirus) DAS-ELISA RT-0858 Posi�ve control PC-0858 Nega�ve control NC-0858 Apium virus Y (ApVY, Potyvirus) DAS-ELISA RT-1001 Posi�ve control PC-1001 Nega�ve control NC-1001 Arabis mosaic virus (ArMV, Nepovirus) DAS-ELISA RT-0008 Posi�ve control PC-0045 Nega�ve control NC-0045 Arracacha virus B (AVB, Cheravirus) DAS-ELISA RT-0009 Posi�ve control PC-0082 Nega�ve control NC-0082 Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Pathogen Test Format Product Code Asparagus virus 1 (AV-1, Potyvirus) DAS-ELISA RT-0954 Posi�ve control PC-0954 Nega�ve control NC-0954 Asparagus virus 2 (AV-2, Ilarvirus) TAS-ELISA RT-0953-0645/1 Posi�ve control PC-0953 Nega�ve control NC-0953 Bacopa chlorosis virus (BaCV, Ilarvirus) DAS-ELISA RT-0991 Posi�ve control PC-0991 Nega�ve control NC-0991 Banana Xanthomonas / bacterial wilt (BXW) see Xanthomonas vasicola pv. musacearum (Xvm) Banana streak Mysore virus (BSMysV, Badnavirus) DAS-ELISA RT-1049 Also available as B-Fast ELISA. B-Fast BF-1049 Posi�ve control PC-1049 Nega�ve control NC-1049 Banana streak OL virus (BSOLV, Badnavirus) TAS-ELISA RT-0986-0492/31 Posi�ve control PC-0492 Nega�ve control NC-0492 Barley mild mosaic virus (BaMMV, Bymovirus) DAS-ELISA RT-0139 Posi�ve control PC-0329 Nega�ve control NC-0329 Barley stripe mosaic virus (BSMV, Hordeivirus) DAS-ELISA RT-0135 Posi�ve control PC-0330 Nega�ve control NC-0330 Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Pathogen Test Format Product Code Barley yellow mosaic virus (BaYMV, Bymovirus) DAS-ELISA RT-0922 Posi�ve control PC-0634 Nega�ve control NC-0634 Bean common mosaic necrosis virus DAS-ELISA RT-0239 (BCMNV, Potyvirus) Posi�ve control PC-0413 Nega�ve control NC-0413 Bean common mosaic virus (BCMV, Potyvirus) TAS-ELISA RT-0915-0228/1 Posi�ve control PC-0463 Nega�ve control NC-0463 Bean golden mosaic virus (BGMV, Begomovirus) TAS-ELISA RT-0616-0616/2 Posi�ve control PC-0094 Nega�ve control NC-0094 Bean leaf roll virus (BLRV, Luteovirus) TAS-ELISA RT-0142-0227/2 Posi�ve control PC-0331 Nega�ve control NC-0331 Bean yellow mosaic virus (BYMV, Potyvirus) DAS-ELISA RT-0717 Posi�ve control PC-0717 Nega�ve control NC-0717 Beet black scorch virus (BBSV, Betanecrovirus) DAS-ELISA RT-0951 Posi�ve control PC-0951 Nega�ve control NC-0951 Beet chlorosis virus (BChV, Polerovirus) and Posi�ve control PC-1211 Beet mild yellowing virus (BMYV, Polerovirus) Posi�ve control PC-1210 can be reliably detected with our Beet western yellows virus (BWYV) ELISA. Leibniz Ins�tute DSMZ-German Collec�on of Microorganisms and Cell Cultures GmbH Pathogen Test Format Product Code Beet necro�c yellow vein virus (BNYVV, Benyvirus) DAS-ELISA RT-0737 Also available as B-Fast ELISA. B-Fast BF-0737 Posi�ve control PC-0467 Nega�ve control NC-0467 Beet soil-borne mosaic virus (BSBMV, Benyvirus) DAS-ELISA RT-1035 Posi�ve control PC-1035 Nega�ve control NC-1035 Beet soil-borne virus (BSBV, Pomovirus) TAS-ELISA RT-0576-0576/2 Weak cross reac�on with BVQ observed. Posi�ve control PC-0576 Nega�ve control NC-0576 Beet western yellows virus (BWYV, Polerovirus) TAS-ELISA RT-0049-0049/1 Also available as B-Fast ELISA. B-Fast BF-0049 All tests also detect Turnip yellows virus (TuYV), Posi�ve control PC-1209 Beet mild yellowing virus (BMYV) and Posi�ve control PC-1210 Beet chlorosis virus (BChV).