Catalog # Aliquot Size M14-911-05 3 x 5 nmol M14-911-20 3 x 20 nmol M14-911-50 3 x 50 nmol MEKK6 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # M14-911 Lot # Z2049-94 Specificity Formulation MEKK6 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human MEKK6 expression. shipped at room temperature. Product Description Reconstitution Protocol MEKK6 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human MEKK6 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_004672. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot MAP3K6, MAPKKK6, ASK2 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or MEKK1, Active M09-11G below -20oC. After resuspension, siRNA stock solutions ≥2 MEKK2, Active M10-10G µM can undergo up to 50 freeze-thaw cycles without MEKK3, Active M11-10G significant degradation. For long-term storage, it is MEKK6, Active M14-11G recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background MEKK6 or MAP3K6 (mitogen-activated protein kinase kinase kinase 6) is a member of the serine/threonine protein kinase family. MEKK6 was identified by its interaction with MAP3K5/ASK, a protein kinase and an MEKK6 siRNA Set I activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 siRNA duplexes targeted against three exon regions kinase (1). MEKK6 cascades relay signals from the cell Catalog Number M14-911 surface to the nucleus which result in gene transcription (2). MEKK6 activates the JNK and p38 pathway and Specific Lot Number Z2049-94 induces apoptosis when overexpressed in stably Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol Format Lyophilized powder transfected cells. MEKK6 transcript is expressed in several Stability 1yr at -70oC from date of shipment tissues, with the highest expression in heart and skeletal Storage & Shipping The lyophilized powder is stable for at muscle. least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Wang, X. S. et al: MAPKKK6, a novel mitogen- siRNA stock solutions ≥2 µM can activated protein kinase kinase kinase, that undergo up to 50 freeze-thaw cycles without significant degradation. For associates with MAPKKK5. Biochem. Biophys. Res. long-term storage, it is recommended Commun. 253: 33-37, 1998. that the siRNA is stored at -70oC. For 2. Takeda, K. et al: Apoptosis signal-regulating kinase most favorable performance, avoid (ASK) 2 functions as a mitogen-activated protein repeated handling and multiple freeze/thaw cycles. kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem. 2007 Mar 9;282(10):7522-31. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .