SAN TA C RUZ BI OTEC HNOL OG Y, INC . LRRN1 (S-17): sc-103020 BACKGROUND SOURCE Leucine-rich repeats (LRRs) are 20-30 amino acid motifs that mediate pro tein- LRRN1 (S-17) is an affinity purified goat polyclonal antibody raised against protein interactions. The primary function of these motifs is to provide a ver - a peptide mapping within an internal region of LRRN1 of human origin. satile structural framework for the formation of these protein-protein inter ac - tions. LRRs are present in a variety of proteins with diverse structure and func - PRODUCT tion, including innate immunity and nervous system development. Several Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide human diseases are associated with mutations in the genes encoding LRR- and 0.1% gelatin. containing proteins. LRRN1 (leucine-rich repeat neuronal protein 1) is a 716 amino acid protein that contains 11 LRRs, one Ig-like C2-type (immuno- glob - Blocking peptide available for competition studies, sc-103020 P, (100 µg ulin-like) domain and one fibronectin type-III domain. The gene encoding this pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). type I transmembrane protein is upregulated by N- and C-Myc and is found to be highly expressed in neuroblastomas that are associated with poor APPLICATIONS prognosis. Knockdown of the LRRN1 gene in cells exhibits reduced growth LRRN1 (S-17) is recommended for detection of LRRN1 of mouse, rat and rate and sensitizes cells to serum starvation. human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range REFERENCES 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1. Taguchi, A., et al. 1996. Molecular cloning of novel leucine-rich repeat 1:30- 1:3000); non cross-reactive with LRRN2 or LRRN3. proteins and their expression in the developing mouse nervous system. LRRN1 (S-17) is also recommended for detection of LRRN1 in additional Brain Res. Mol. Brain Res. 35: 31-40. species, including bovine. 2. Ohira, M., et al. 2003. Neuroblastoma oligo-capping cDNA project: toward Suitable for use as control antibody for LRRN1 siRNA (h): sc-78350, LRRN1 the understanding of the genesis and biology of neuroblastoma. Cancer siRNA (m): sc-149116, LRRN1 shRNA Plasmid (h): sc-78350-SH, LRRN1 shRNA Lett. 197: 63-68. Plasmid (m): sc-149116-SH, LRRN1 shRNA (h) Lentiviral Particles: sc-78350-V 3. Clark, H.F., et al. 2003. The secreted protein discovery initiative (SPDI), a and LRRN1 shRNA (m) Lentiviral Particles: sc-149116-V. large-scale effort to identify novel human secreted and transmembrane Molecular Weight of LRRN1: 81 kDa. proteins: a bioinformatics assessment. Genome Res. 13: 2265-2270. 4. Higgins, J.J., et al. 2004. Candidate genes for recessive non-syndromic RECOMMENDED SECONDARY REAGENTS mental retardation on chromosome 3p (MRT2A). Clin. Genet. 65: 496-500. To ensure optimal results, the following support (secondary) reagents are 5. Hamano, S., et al. 2004. Identification of novel human neuronal leucine- rich recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 repeat (hNLRR) family genes and inverse association of expression of (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey Nbla10449/hNLRR-1 and Nbla10677/hNLRR-3 with the prognosis of pri mary anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ neuroblastomas. Int. J. Oncol. 24: 1457-1466. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- 6. Haines, B.P., et al. 2005. The NLRR gene family and mouse development: rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- Modified differential display PCR identifies NLRR-1 as a gene expressed 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) in early somitic myoblasts. Dev. Biol. 281: 145-159. with UltraCruz™ Mounting Medium: sc-24941. 7. García-Calero, E., et al. 2006. Expression of Lrrn1 marks the prospective site of the zona limitans thalami in the early embryonic chicken dien cephalon. STORAGE Gene Expr. Patterns 6: 879-885. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of 8. Andreae, L.C., et al. 2007. Analysis of Lrrn1 expression and its relationship shipment. Non-hazardous. No MSDS required. to neuromeric boundaries during chick neural development. Neural Dev. 2: 22. RESEARCH USE For research use only, not for use in diagnostic procedures. 9. Hossain, M.S., et al. 2008. N-MYC promotes cell proliferation through a direct transactivation of neuronal leucine-rich repeat protein-1 (NLRR1) gene in neuroblastoma. Oncogene 27: 6075-6082. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and CHROMOSOMAL LOCATION support products. Genetic locus: LRRN1 (human) mapping to 3p26.2; Lrrn1 (mouse) mapping to 6 E1. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.