OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for MG226706 Med14 (NM_001048208) Mouse Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: Med14 (NM_001048208) Mouse Tagged ORF Clone Tag: TurboGFP Symbol: Med14 Synonyms: 9930001L01Rik; AU041628; Crsp2; ENSMUSG00000073278; Gm641; ORF1; Trap170 Vector: pCMV6-AC-GFP (PS100010) E. coli Selection: Ampicillin (100 ug/mL) Cell Selection: Neomycin ORF Nucleotide >MG226706 representing NM_001048208 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCAGTGCAGCTGGACAACCACCAGCTGATCCCTCCTGGCGGCGGCGGTGGCAGCAGCGGCGGCG GAGGCAGCAGCAGCGGCTCCGCGTCTGCCCCGGCTCCCCCGCCGCCCGCAGCCGCTGTGGCGGCGGCAGC TGCGGCGGCAGCTAGCCCTGGATACCGGCTTAGCACGCTCATCGAATTTCTGCTCCACCGGGCCTACTCG GAGCTTATGGTGTTGACGGACTTATTGCCAAGGAAATCTGATGTGGAAAGGAAAATAGAAATTGTACAGT TTGCTAGCCGGACACGTCAACTCTTCGTTCGATTATTAGCTTTAGTAAAATGGGCCAATGACGCTGGCAA AGTAGAAAAGTGTGCGATGATCTCAAGCTTTTTAGATCAGCAAGCTATCTTATTTGTGGATACCGCTGAC CGCTTGGCCTCCTTAGCTAGAGATGCCCTGGTCCATGCACGCCTGCCTAGTTTTGCTATTCCATATGCCA TTGATGTACTGACTACTGGCTCTTATCCACGGCTTCCAACCTGCATCAGGGACAAAATTATTCCTCCAGA CCCAATTACCAAAATTGAGAAACAAGCCACACTTCATCAGCTTAATCAGATTCTTAGACATAGGCTTGTA ACGACAGATCTTCCTCCACAGCTAGCAAATCTTACAGTGGCAAATGGCCGTGTGAAGTTTCGAGTTGAAG GAGAATTTGAAGCAACCTTGACAGTGATGGGTGATGACCCAGAAGTCCCGTGGCGTCTTCTCAAGCTAGA AATTCTAGTTGAGGATAAGGAAACAGGAGATGGGCGAGCTTTGGTTCATAGCATGCAAATCGATTTTATC CATCAGCTGGTCCAGTCTAGGCTCTTTGCTGATGAGAAACCTCTTCAGGACATGTACAACTGCCTACATT GTTTCTGCTTATCGCTTCAACTAGAAGTATTACATTCCCAGACACTAATGTTAATCCGAGAGAGGTGGGG AGACCTTGTACAGGTGGAAAGATACCATGCTGGAAAGAGCCTCTCCCTCTCAGTTTGGAATCAACAGGTT CTAGGGAGAAAAACAGGCACAGCATCTGTTCACAAAGTTACAATTAAAATCGATGAGAATGATGTCTCCA AGCCTTTACAGATTTTTCATGATCCTCCTTTGCCAGCTTCTGATTCTAAATTAGTAGAAAGAGCCATGAA GATTGACCACTTATCAATAGAAAAGCTCCTGATTGACAGTGTACACGCAAGAGCCCACCAGAGGCTGCAG GAACTGAAGGCCATTCTTAGAAGCTTCAACGCCAATGAAAGCTCTTCCATAGAGACTGCACTTCCAGCTC TTATTGTACCCATCTTGGAGCCTTGTGGTAATTCGGAGTGCCTACACATTTTTGTAGATTTGCATTCTGG AATGTTCCAATTGATGCTTTATGGACTTGACCCAGCCACTCTGGAGGACATGGAAAAATCTTTGAATGAT GACATGAAGCGGATCATCCCTTGGATTCAGCAACTTAAGTTTTGGCTGGGACAACAGCGTTGCAAACAAT This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 Med14 (NM_001048208) Mouse Tagged ORF Clone – MG226706 CTATAAAACATCTGCCTACAATAACCACTGAAACGTTGCAGCTTGCCAACTATTCAACACATCCCATTGG AAGCCTTTCTAAGAATAAGCTGTTTATTAAACTTACTCGCCTTCCACAGTACTACATTGTTGTGGAAATG CTTGAGGTTCCTAATAAGCCTACGCAGCTATCATATAACTATTACTTTATGTCGGTGAGTACTGCAGATC GTGAGGACAGCCCTGTCATGGCACTGCTGCTGCAGCAATTCAAGGACAACATCCAGGACCTGATGTCCTA TACAAAGACTGGGAAACAGACCAGAACTGGTACCAAGCACAAGTTGTCTGATGACCCATGTCCGATAGAC TCCAAGAAAGCCAAACGATCAGGAGAAATGTGTGCCTTCAATAAAGTTCTAGCTCACTTTGTTGCTATGT GTGACACCAACATGCCATTTGTTGGTCTTCGATTGGAGTTGTCTAACCTCGAGATACCACATCAGGGAGT GCAAGTGGAAGGTGATGGCTTCAACCATGCAATTCGCTTATTAAAGATTCCTCCCTGTAAAGGAATAAGT GAGGAAACACAGAAGGCTCTGGACCGCTCTCTTCTTGATTGCACTTTCCGATTACAAGGTAGAAATAACC GCACTTGGGTGGCTGAATTAGTGTTTGCAAATTGTCCGCTTAATGGCACTTCCACCAGGGAGCAAGGACC ATCCCGACATGTTTACCTGACGTATGAAAATTTGTTGTCTGAACCTGTTGGTGGCAGAAAAGTAGTTGAA ATGTTTCTTAATGACTGGAGTAGCATTGCCCGTTTATACGAGTGTGTGTTGGAATTTGCACGTTCTCTAC CAGAAATACCTGCTCATCTGAATATTTTCTCAGAAGTTCGTGTTTATAATTATCGAAAGCTTATCTTATG TTATGGAACCACCAAAGGAAGCTCAATTAGTATTCAGTGGAACTCCATTCATCAGAAATTTCACATTGCG TTGGGAACAGTTGGCCCAAACTCAGGTTGCAGTAACTGTCACAATACCATTCTCCATCAACTTCAAGAAA TGTTCAACAAAACACCAAATGTGGTTCAGTTATTACAGGTTTTGTTTGATACTCAGGCACCATTAAATGC TATCAACAAACTCCCTACTGTTCCAATGTTGGGCTTGACCCAGAGAACTAACACCGCCTACCAGTGTTTC TCCATTTTACCACAATCATCCACCCACATCAGACTGGCCTTCAGGAACATGTACTGCATTGACATATACT GCCGTAGTCGAGGCGTCGTGGCAATACGGGACGGTGCCTATAGTCTTTTTGATAACAGCAAGTTAGTTGA AGGCTTCTATCCTGCACCAGGATTAAAGACTTTCCTGAACATGTTTGTTGATAGCAATCAAGATGCTCGG AGAAGATCTGTAAATGAGGATGATAACCCTCCTTCTCCTATAGGAGGTGACATGATGGACTCTTTAATAT CACAGCTCCAGCCACCTCAGCAACAGCCATTTCCAAAGCAGCCAGGGACATCAGGCGCTTACCCTCTTAC TTCACCCCCTACATCCTATCACAGCACAGTTAATCAGTCCCCCTCTATGATGCACACACAGTCTCCAGGA AATCTGCATGCTGCCAGCTCCCCCAGTGGGGCTTTGAGAGCCCCATCACCAGCGTCATTTGTTCCAACTC CTCCCCCATCCTCGCATGGAATCTCAATAGGACCAGGGGCCAGTTTTGCTAGTCCACATGGAACCCTTGA CCCTAGTTCTCCATATACTATGGTGTCACCAAGTGGACGAGCAGGAAACTGGCCTGGGTCACCTCAAGTG TCCGGACCCTCACCAGCAACCCGTTTGCCTGGAATGTCACCAGCTAACCCATCTCTGCATTCTCCTGTTC CAGATGTTTCTCATTCTCCTCGAGCTGGCACAAGTTCCCAGACAATGCCAACCAACATGCCTCCACCTCG AAAACTACCTCAGCGCTCCTGGGCAGCATCTATACCCACCATTCTCACTCATAGTGCCTTGAACATCTTA CTGCTACCATCTCCAACGCCAGGCCTTGTGCCTGGCCTGGCAGGTAGTTACCTTTGTTCCCCACTTGAGA GATTCCTTGGATCTGTCATCATGAGACGACACCTTCAAAGAATCATCCAACAGGAAACGTTGCAGCTGAT CAATTCCAATGAACCTGGTGTAATCATGTTCAAGACAGATGCCCTGAAATGCAGAGTAGCCCTTAGTCCC AAAACCAACCAGACCCTTCAGCTAAAAGTGACACCTGAAAATGCAGGACAATGGAAACCCGATGAGCTTC AAGTTTTGGAGAAATTCTTCGAAACAAGAGTTGCAGGACCGCCATTTAAAGCCAACACACTTATAGCCTT CACCAAGCTGTTGGGAGCGCCGACACACATCCTCCGGGACTGTGTGCATATAATGAAGTTGGAGTTGTTC CCAGATCAAGCAACGCAGCTGAAATGGAACGTTCAGTTTTGCCTCACCATCCCTCCCAGCGCACCACCAA TTGCACCGCCTGGGACACCAGCTGTGGTCCTGAAATCCAAAATGCTATTTTTTCTTCAACTAACTCAGAA AACATCAGTCCCTCCCCAAGAACCTGTTAGTATAATAGTTCCAATTATTTATGATATGGCTTCAGGTACC ACTCAGCAGGCAGACATTCCCAGACAGCAGAACTCTTCTGTTGCTGCGCCAATGATGGTCAGCAACATTC TGAAGAGGTTTGCAGAGATGAACCCTCCACGACAAGGTGAATGCACAATATTTGCAGCTGTTCGTGATTT AATGGCTAATCTTACACTGCCCCCTGGTGGGCGTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 Med14 (NM_001048208) Mouse Tagged ORF Clone – MG226706 Protein Sequence: >MG226706 representing NM_001048208 Red=Cloning site Green=Tags(s) MAPVQLDNHQLIPPGGGGGSSGGGGSSSGSASAPAPPPPAAAVAAAAAAAASPGYRLSTLIEFLLHRAYS ELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANDAGKVEKCAMISSFLDQQAILFVDTAD RLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLV TTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPEVPWRLLKLEILVEDKETGDGRALVHSMQIDFI HQLVQSRLFADEKPLQDMYNCLHCFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKSLSLSVWNQQV LGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQRLQ ELKAILRSFNANESSSIETALPALIVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDPATLEDMEKSLND DMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITTETLQLANYSTHPIGSLSKNKLFIKLTRLPQYYIVVEM LEVPNKPTQLSYNYYFMSVSTADREDSPVMALLLQQFKDNIQDLMSYTKTGKQTRTGTKHKLSDDPCPID SKKAKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFNHAIRLLKIPPCKGIS EETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVE MFLNDWSSIARLYECVLEFARSLPEIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHIA LGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCF SILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDAR RRSVNEDDNPPSPIGGDMMDSLISQLQPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPG NLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQV SGPSPATRLPGMSPANPSLHSPVPDVSHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNIL LLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSP KTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELF PDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGT TQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP TRTRPLE - GFP Tag - V Restriction Sites: SgfI-MluI Cloning Scheme: This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 Med14 (NM_001048208) Mouse Tagged ORF Clone – MG226706 Plasmid Map: ACCN: NM_001048208 ORF Size: 4377 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_001048208.1, NP_001041673.1 RefSeq Size: 6861 bp RefSeq ORF: 4380 bp This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 Med14 (NM_001048208) Mouse Tagged ORF Clone – MG226706 Locus ID: 26896 UniProt ID: A2ABV5 Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). [UniProtKB/Swiss-Prot Function] This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 5 / 5.