US008784836B2 (12) UnitedO States Patent (10) Patent No.: US 8,784,836 B2 Szalay et al. (45) Date of Patent: *Jul. 22, 2014 (54) MICROORGANISMS FORTHERAPY 5,221,623 A 6/1993 Legocki et al. ............ 435/252.3 5,283,187 A 2f1994 Aebischer et al. ... 435/182 (75) Inventors: Aladar A. Szalay, Highland, CA (US). 5,364,7735,300,436 A 1 4,1/1994 1994 PaolettiGoldstein et etal. al. ..... .. ... 435/69.1435/190 Tatyana Timiryasova, San Diego, CA 5,368,855. A 1 1/1994 Boyle et al. ................ 435/320.1 (US); Yong A. Yu, San Diego, CA (US); 5,378.457 A 1/1995 Paoletti et al. ............. 424/205.1 Qian Zhang, San Diego, CA (US) 5,494,807 A 2f1996 Paoletti .......... ... 435/69.3 5,550,050 A 8, 1996 Holland et al. ... 435/.382 (73) Assignee: Genelux Corporation, San Diego, CA 5,639,275 A 6/1997 Baetge et al. ...... 604/891.1 (US) 5,646.298 A 7/1997 Powell et al. ...... ... 548/427 5,650,135 A 7/1997 Contaget al. .. ... 424/9.1 - 5,650,148 A 7/1997 Gage et al. ..... ... 424/93.2 (*) Notice: Subject to any disclaimer, the term of this 5,653,975 A 8/1997 Baetge et al. .. ... 424,931 patent is extended or adjusted under 35 5,656,481 A 8/1997 Baetge et al. ...... 435/325 U.S.C. 154(b) by 0 days. 5,676,943 A 10/1997 Baetge et al. .. 424.93.21 5,693,533 A 12/1997 Raney et al. ... ... 435,366 This patent is Subject to a terminal dis- 5,704,910 A 1/1998 Humes ........ ... 604,502 claimer. 5,710,137 A 1/1998 Fisher ............................. 514,44 5,718,902 A 2f1998 Yilma et al. ... 424/211.1 (21) Appl. 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No. 8,221,769, which is a 5,840,576 A 11/1998 Schinstine et al. 435/325 continuation of application No. 1 1/796,028, filed on 5,842,431 A 12/1998 Wu ............................... 112,232 Apr. 25, 2007, which is a division of application No. (Continued) 10/872,156, filed on Jun. 18, 2004, now Pat. No. 7,588,767. FOREIGN PATENT DOCUMENTS (30) Foreign Application Priority Data AU TO9336 4f1995 CA 2105.277 12/2006 Jun. 18, 2003 (EP) ..................................... O3O13826 (Continued) Aug. 14, 2003 (EP) ..................................... O3O18478 Oct. 22, 2003 (EP) ..................................... O3O24283 OTHER PUBLICATIONS (51) Int. Cl. Neubauer et al., Specific detection of monkeypox virus by AOIN 63/00 (2006.01) polymerase chain reaction, 1998, Journal of Virological Methods, A6 IK 39/2 (2006.01) vol. 74, pp. 201-207.* A 6LX39/275 (2006.01) A6 IK39/00 (2006.01) (Continued) GOIN33/574 (2006.01) A61 K39/145 (2006.01) (52) U.S. Cl. Primary Examiner — Benjamin P Blumel USPC .................. 424/199.1; 424/232.1; 424/205.1; (74) Attorney, Agent, or Firm — McKenna Long & Alridge 424/93.3; 424/277.1 LLP; Stephanie Seidman (58) Field of Classification Search None See application file for complete search history. (57) ABSTRACT (56) References Cited Therapeutic methods and vaccinia virus therefor are pro vided. The viruses are designed so that they accumulate in U.S. PATENT DOCUMENTS immunoprivileged tissues and cells, such as in tumors and 4,216,226 A 8/1980 Fukuyasu et al. ............. 514,546 other proliferating tissue and in inflamed tissues, compared to 4,315,914 A 2, 1982 Arakawa et al. ... 424,281.1 other tissues, cells and organs, so that they exhibit relatively 4,442,203 A 4, 1984 Varshavsky ....................... 435/6 low toxicity to host organisms. 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