(12) United States Patent (10) Patent No.: US 9,452.222 B2 Kraynov Et Al

(12) United States Patent (10) Patent No.: US 9,452.222 B2 Kraynov Et Al

USOO9452222B2 (12) United States Patent (10) Patent No.: US 9,452.222 B2 Kraynov et al. (45) Date of Patent: Sep. 27, 2016 (54) NUCLEIC ACIDS ENCODING MODIFIED 4,680,338 A 7, 1987 Sundoro RELAXN POLYPEPTIDES 4,689,406 A 8, 1987 Banks et al. 4,699,784. A 10, 1987 Shih et al. 4,738,921 A 4, 1988 Belagaje et al. (71) Applicant: Ambrx, Inc., La Jolla, CA (US) 4,755.465 A 7/1988 Gray et al. 4,837,148 A 6/1989 Cregg (72) Inventors: Vadim Kraynov, San Diego, CA (US); 4,859,600 A 8/1989 Gray et al. Nick Knudsen, San Diego, CA (US); 4,876, 197 A 10, 1989 Burke et al. 4,880,734 A 11/1989 Burke et al. Ahma Hewet, Chula Vista, CA (US); 4.902,502 A 2f1990 Nitecki et al. Kristine De Dios, San Diego, CA (US); 4,904,584 A 2, 1990 Shaw Jason Pinkstaff, Encinitas, CA (US); 4,929,555 A 5/1990 Cregg et al. Lorraine Sullivan, San Diego, CA 5,021,234 A 6, 1991 Ehrenfeld (US) 5,089,398 A 2/1992 Rosenberg et al. 5,122,614 A 6/1992 Zalipsky 5,145,962 A 9, 1992 Hudson et al. (73) Assignee: AMBRX, INC., La Jolla, CA (US) 5,162,601 A 11/1992 Slightom 5,219,564 A 6/1993 Zalipsky et al. (*) Notice: Subject to any disclaimer, the term of this 5,229,490 A 7, 1993 Tam patent is extended or adjusted under 35 5,231,178 A 7, 1993 Holtz et al. U.S.C. 154(b) by 0 days. 5,252,714 A 10, 1993 Harris et al. 5,281,698 A 1/1994 Nitecki 5,290,686 A 3, 1994 Kendal et al. (21) Appl. No.: 14/152,302 5,324,639 A 6/1994 Brierley et al. 5,324,844 A 6/1994 Zalipsky (22) Filed: Jan. 10, 2014 5,382,657 A 1/1995 Karasiewicz et al. 5,446,090 A 8, 1995 Harris (65) Prior Publication Data 5,468.478 A 11/1995 Saifer et al. 5,473,034 A 12/1995 Yasui et al. US 2014/O194357 A1 Jul. 10, 2014 5,476,653 A 12, 1995 Pitt et al. 5,516,657 A 5/1996 Murphy et al. 5,516,673 A 5/1996 Margel et al. Related U.S. Application Data 5,532,142 A 7/1996 Johnston et al. 5,559,213 A 9, 1996 Hakimi et al. 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Adams et al., “Molecular cloning of mouse immunoglobulin heavy (56) References Cited chain messenger ribonucleic acids coding for mu, alpha, gamma 1. gamma 2a, and gamma 3 chains’. Biochemistry (1980) U.S. PATENT DOCUMENTS 19(12):2711-2719. (Continued) 3,773.919 A 11/1973 Boswell et al. 4,289,872 A 9, 1981 Denkewalter et al. 4.412,989 A 11, 1983 Iwashita et al. 4.414,148 A 11, 1983 Jansen et al. Primary Examiner — Christine J Saoud 4.485,045 A 11/1984 Regen (74) Attorney, Agent, or Firm — LeClairRyan, a 4,511,502 A 4, 1985 Builder et al. professional corporation; Robin L. Teskin 4,511,503 A 4, 1985 Olson et al. 4,512.922 A 4, 1985 Jones et al. 4,544.545 A 10/1985 Ryan et al. (57) ABSTRACT 4,551,433 A 11, 1985 DeBoer 4,569,789 A 2f1986 Blattler et al. Modified relaxin polypeptides and their uses thereof are 4,619,794. 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