hPlexin A4 VersaClone cDNA Catalog Number: RDC1214 Specifications: Description This shuttle vector contains the complete ORF for the gene of interest, Gene: hPLXNA4 along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: NP_065962 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 5697bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5 Shipping Ships at ambient temperature Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated hPlexin A4 cDNA freeze-thaw cycles. Plasmid PLXNA4 plexin A4 [ Homo sapiens (human) ] HpaI HindIII SspI BamHI Also known as: PLEXA4; PLXNA4A; BmtI NheI NotI PLXNA4B; FAYV2820; PRO34003 EagI BsiWI Summary: AMP EcoNI PLXNA4 is a member of the Plexin family of semaphorin signal transducers. It is found on sensory, COLE1 PshAI autonomic and motor neurons and oligodendrocytes, plus T cells and RDC1214 dendritic cells. PLXNA4 regulates cell 8431 bps Eco47III migration, activation, and axon XhoI guidance via repulsion. It serves as a PspXI SalI receptor for transmembrane XbaI BssHII NsiI semaphorins, Sema6A and 6B, and AscI hPlexin-A4 (1-1894) as a coreceptor with neuropilin 1 for the secreted semaphorin, Sema3A. NruI SphI Alternatively spliced transcripts AccIII encoding different proteins have been described. Eco53kI SacI BsrGI FOR RESEARCH USE ONLY NOT FOR USE IN HUMANS Page 1 of 3 > RDC1214 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atgaaagcca tgccctggaa ctggacctgc cttctctccc acctcctcat 501 ggtgggcatg ggctcctcca ctttgctcac ccggcagcca gccccgctgt cccagaagca gcggtcattt gtcacattcc gaggagagcc cgccgagggt 601 ttcaatcacc tggtggtgga tgagaggaca ggacacattt acttgggggc cgtcaatcgg atttacaagc tctccagcga cctgaaggtc ttggtgacgc 701 atgagacagg gccggacgag gacaacccca agtgttaccc accccgcatc gtccagacct gcaatgagcc cctgaccacc accaacaatg tcaacaagat 801 gctcctcata gactacaagg agaacaggct gattgcctgt gggagcctgt accaaggcat ctgcaagctg ctgaggctgg aggacctctt caagctgggg 901 gagccttatc ataagaagga gcactatctg tcaggtgtca acgagagcgg ctcggtcttt ggagtgatcg tctcctacag caacctggat gacaagctgt 1001 tcattgccac ggcagtggat gggaagcccg agtattttcc caccatctcc agccggaaac tgaccaagaa ctctgaggcg gatggcatgt tcgcgtacgt 1101 cttccatgat gagttcgtgg cctcgatgat taagatccct tcggacacct tcaccatcat ccctgacttt gatatctact atgtctatgg ttttagcagt 1201 ggcaactttg tctacttttt gaccctccaa cctgagatgg tgtctccacc aggctccacc accaaggagc aggtgtatac atccaagctc gtgaggcttt 1301 gcaaggagga cacagccttc aactcctatg tagaggtgcc cattggctgt gagcgcagtg gggtggagta ccgcctgctg caggctgcct acctgtccaa 1401 agcgggggcc gtgcttggca ggacccttgg agtccatcca gatgatgacc tgctcttcac cgtcttctcc aagggccaga agcggaaaat gaaatccctg 1501 gatgagtcgg ccctgtgcat cttcatcttg aagcagataa atgaccgcat taaggagcgg ctgcagtctt gttaccgggg cgagggcacg ctggacctgg 1601 cctggctcaa ggtgaaggac atcccctgca gcagtgcgct cttaaccatt gacgataact tctgtggcct ggacatgaat gctcccctgg gagtgtccga 1701 catggtgcgt ggaattcccg tcttcacgga ggacagggac cgcatgacgt ctgtcatcgc atatgtctac aagaaccact ctctggcctt tgtgggcacc 1801 aaaagtggca agctgaagaa gatccgggtg gatggaccca ggggcaacgc cctccagtat gagacggtgc aggtggtgga ccccggccca gtcctccggg 1901 atatggcctt ctccaaggac cacgagcaac tctacatcat gtcagagagg cagctcacca gagtccctgt ggagtcctgt ggtcagtatc agagctgcgg 2001 cgagtgcctt ggctcaggcg acccccactg tggctggtgt gtgctgcaca acacttgcac ccggaaggag cggtgtgagc ggtccaagga gccccgcagg 2101 tttgcctcgg agatgaagca gtgtgtccgg ctgacggtcc atcccaacaa tatctccgtc tctcagtaca acgtgctgct ggtcctggag acgtacaatg 2201 tcccggagct gtcagctggc gtcaactgca cctttgagga cctgtcagag atggatgggc tggtcgtggg caatcagatc cagtgctact cccctgcagc 2301 caaggaggtg ccccggatca tcacagagaa tggggaccac catgtcgtac agcttcagct caaatcaaag gagaccggca tgaccttcgc cagcaccagc 2401 tttgtcttct acaattgcag cgtccacaat tcgtgcctgt cctgcgtgga gagtccatac cgctgccact ggtgtaaata ccggcatgtc tgcacccatg 2501 accccaagac ctgctccttc caggaaggcc gagtgaagct gcccgaggac tgcccccagc tgctgcgagt ggacaagatc ctggtgcccg tggaggtgat 2601 caagcctatc acgctgaagg ccaagaacct cccccagccc cagtctgggc agcgtggcta cgaatgcatc ctcaacattc agggcagcga gcagcgagtg 2701 cccgccctgc gcttcaacag ctccagcgta cagtgccaga acacctctta ttcctatgaa gggatggaga tcaacaacct gcccgtggag ttgacagtcg 2801 tgtggaatgg gcacttcaac attgacaacc cagctcagaa taaagttcac ctctacaagt gtggagccat gcgtgagagc tgcgggctgt gcctcaaggc 2901 tgacccagac ttcgcatgtg gctggtgcca gggcccaggc cagtgcaccc tgcgccagca ctgccctgcc caggagagcc agtggctgga gctgtctggt 3001 gccaaaagca agtgcacaaa cccccgcatc acagagataa tcccggtgac aggcccccgg gaagggggca ccaaggtcac tatccgaggg gagaacctgg 3101 gcctggaatt tcgcgacatc gcctcccatg tcaaggttgc tggcgtggag tgcagccctt tagtggatgg ttacatccct gcagaacaga tcgtgtgtga 3201 gatgggggag gccaagccca gccagcatgc aggcttcgtg gagatctgcg tggctgtgtg tcggcctgaa ttcatggccc ggtcctcaca gctctattac 3301 ttcatgacac tgactctctc agatctgaag cccagccggg ggcccatgtc cggagggacc caagtgacca tcacaggcac caacctgaat gccggaagca 3401 acgtggtggt gatgtttgga aagcagccct gtctcttcca caggcgatct ccatcctaca ttgtctgcaa caccacatcc tcagatgagg tgctagagat 3501 gaaggtgtcg gtgcaggtgg acagggccaa gatccaccag gacctggtct ttcagtatgt ggaagacccc accatcgtgc ggattgagcc agaatggagc 3601 attgtcagtg gaaacacacc catcgccgta tgggggaccc acctggacct catacagaac ccccagatcc gtgccaagca tggagggaag gagcacatca 3701 atatctgtga ggttctgaac gctactgaga tgacctgtca ggcgcccgcc ctcgctctgg gtcctgacca ccagtcagac ctgaccgaga ggcccgagga 3801 gtttggcttc atcctggaca acgtccagtc cctgctcatc ctcaacaaga ccaacttcac ctactatccc aacccggtgt ttgaggcctt tggtccctca 3901 ggaatcctgg agctcaagcc tggcacgccc atcatcctaa agggcaagaa cctgatcccg cctgtggctg ggggcaacgt gaagctgaac tacactgtgc 4001 tggttgggga gaagccgtgc accgtgaccg tgtcagatgt ccagctgctc tgcgagtccc ccaacctcat cggcaggcac aaagtgatgg cccgtgtcgg 4101 tggcatggag tactccccgg ggatggtgta cattgccccg gacagcccgc tcagcctgcc cgccatcgtc agcatcgcag tggctggcgg cctcctcatc 4201 attttcatcg tggccgtgct cattgcctat aaacgcaagt cccgcgaaag tgacctcacg ctgaagcggc tgcagatgca gatggacaac ctggagtccc 4301 gtgtggccct ggagtgcaag gaagcctttg ccgagctgca gacggacatc catgagctga ccagtgacct ggatggagcc gggattccgt tcctggacta 4401 tagaacttac accatgcggg tgctgttccc aggaattgaa gaccaccctg tcctccggga ccttgaggtc ccgggctacc ggcaggagcg tgtggagaaa 4501 ggcctgaagc tcttcgccca gctcatcaac aacaaggtgt tcctgctgtc cttcatccgc acgcttgagt cccagcgtag cttctccatg cgcgaccgtg 4601 gcaacgtggc ctcactcatc atgaccgtgc tgcagagcaa gctggagtac gccactgatg tgctgaagca gctgctggcc gacctcattg acaagaacct 4701 ggagagcaag aaccacccta agctgctgct caggaggact gagtcagtgg ctgagaagat gctgaccaat tggtttactt tcctcctcta caagttcctc 4801 aaggagtgtg ctggggagcc cctcttctcc ctgttctgtg ccatcaagca gcagatggag aagggcccca ttgacgccat cacgggcgag gcccgctact 4901 ccttgagcga ggacaagctc atccgccagc agattgacta caaaaccctg gtcctgagct gtgtcagccc agacaatgcc aacagccccg aggtcccagt 5001 aaagatcctc aactgtgaca ccatcactca ggtcaaggag aagattctgg atgccatctt caagaatgtg ccttgctccc accggcccaa agctgcagat 5101 atggatctgg agtggcgaca aggaagtggg gcaaggatga tcttgcagga tgaagacatc accaccaaga ttgagaatga ttggaagcga ctgaacacac 5201 tggcccacta ccaggtgcca gatggttccg tggtggcatt agtgtccaag caggtgacag cctataacgc agtgaacaac tccaccgtct ccaggacctc 5301 agcaagtaaa tatgaaaaca tgatccggta cacgggcagc cccgacagcc tccgctcacg gacacctatg atcactcctg acctggagag tggagtcaag 5401 atgtggcacc tagtgaagaa ccacgagcac ggagaccaga aggaggggga ccgggggagc aagatggtgt ctgaaatcta cctgacccga ctcctggcca 5501 ctaagggcac actgcagaag tttgtggatg acctctttga gaccatcttc agcacggcac accgtggctc tgccctgccc ctggccatca agtacatgtt 5601 tgacttcctg gatgagcagg ctgataaaca tggcattcat gacccgcacg tccgccatac ctggaagagc aattgcctgc ccctgaggtt ttgggtcaac 5701 atgatcaaga acccgcagtt tgtgtttgac atccataaga acagcatcac agacgcctgc ctctctgtgg tggctcagac cttcatggac tcttgctcca 5801 cgtcagagca ccggctgggc aaggactcgc cctccaacaa gctgctgtat gccaaggaca tccccagcta caagaattgg gtggagaggt attactcaga 5901 catagggaag atgccagcca tcagcgacca agacatgaac gcatacctgg ctgagcagtc ccggatgcac atgaatgagt tcaacaccat gagtgcactc 6001 tcagagatct tctcctatgt gggcaaatac agcgaggaga tccttggacc tctggaccac gatgaccagt gtgggaagca gaaactggcc tacaaactag 6101 aacaagtcat aaccctcatg agcttagaca gctgaggcgc gccagtatac tctagagtcg acacccgggg aattcctcga gcgctcgtct ctagcttggc 6201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 6301 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6401 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 6601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6701 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6801 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 6901 ttatccggta