OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP08426PU-N Polyclonal Antibody to APIP (full length) - Purified Alternate names: APAF1-interacting protein, CGI-29 Quantity: 50 µg Concentration: 0.5 mg/ml Background: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase 9 (Apaf3), which leads to caspase 9 activation. Activated caspase 9 in turn cleaves and activates caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. A new Apaf1 Interacting Protein (APIP) also known as CG129 and MMRP19, has been identified as a negative regulator of ischemic injury. APIP competes with Caspase 9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney. Uniprot ID: Q96GX9 NCBI: NP_057041.2 GeneID: 51074 Host / Isotype: Rabbit / IgG Immunogen: Recombinant protein corresponding to a full-length His-tagged recombinant Human APIP protein Format: State: Liquid purified Ig fraction Purification: Protein G Chromatography Buffer System: PBS containing 0.2% Gelatin as stabilizer and 0.05% Sodium Azide as preservative Applications: Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 1 - 2 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes APIP. Species Reactivity: Tested: Human Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AP08426PU-N: Polyclonal Antibody to APIP (full length) - Purified Pictures: Kidney: Formalin-Fixed Paraffin- Embedded (FFPE) For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2 Powered by TCPDF (www.tcpdf.org).