SEKK SUMMARY STATISTICS EQA round: NF2/17 - Peripheral Blood Smears - Photos Dead line (EQA round closed): 29.09.2017 Professional Czech Haematological Society supervision: National Reference Laboratory for Haematology Key: >>> ... marks correct (expected) result > ... marks conditionally correct (acceptable) result Photo 1 Object no.1WBC and other nucleous elements - types of cells 1 Neutrophil metamyelocyte 1,8 % >>> 52 Neutrophil bar 95 % 2 Neutrophil segment 3,6 % WBC - morphology 6 No changes 11 % >>> 39 Hypergranulation/toxic granulation 71 % 5 Giant bands and metamyelocytes 9,1 % >>> 17 Döhle bodies 31 % >>> 29 Vacuolisation 53 % Object no.2WBC and other nucleous elements - types of cells >>> 55 Neutrophil segment 100 % WBC - morphology >>> 43 No changes 78 % 6 Hypergranulation/toxic granulation 11 % 5 Hypersegmented granulocytes 9,1 % 1 Nucleocytoplasmatic asynchrony 1,8 % RBC - morphology 3 Microcytosis 5,5 % 7 Macrocytosis 13 % >>> 54 Anisocytosis 98 % 3 Poikilocytosis 5,5 % 1 Eliptocytes, ovalocytes 1,8 % >>> 52 Spherocytes 95 % >>> 40 Stomatocytes 73 % >>> 53 Dacryocytes 96 % 1 Target cells 1,8 % 2 Schistocytes (and other fragmentocytes) 3,6 % 4 Polychromasia 7,3 % > 22 Basophilic stippling 40 % > 39 Howell-Jolly bodies 71 % 1 Rouleaux formation 1,8 % > 20 Pappenheimer bodies 36 % >>> 26 Anisochromia 47 % 1 Knizocytes 1,8 % Platelets - morphology 10 Anisocytosis 18 % >>> 55 Platelet aggregates 100 % 1 Platelet satellitism 1,8 % 5 Platelets hypogranulation 9,1 % Photo 2 Object no.1WBC and other nucleous elements - types of cells 3 Blast 5,5 % 4 Lymphocyte 7,3 % >>> 45 Bare nuclei, smudge cell 82 % 1 Erythroblast 1,8 % WBC - morphology 7 No changes 13 % 1 Nucleocytoplasmatic asynchrony 1,8 % 5 Lymphocytes - atypical forms 9,1 % Object no.2WBC and other nucleous elements - types of cells 1 Blast 1,8 % >>> 54 Lymphocyte 98 % WBC - morphology >>> 26 No changes 47 % 2 Lymphocytes - reactive forms 3,6 % 25 Lymphocytes - atypical forms 45 % RBC - morphology 1 Normocytosis 1,8 % 7 Microcytosis 13 % 1 Macrocytosis 1,8 % Printed 10.10.2017, summary evaluation of the results should be found at http://www.sekk.cz Page: 1 SEKK NF2/17 - Peripheral Blood Smears - Photos Photo 2 RBC - morphology >>> 31 Anisocytosis 56 % >>> 46 Poikilocytosis 84 % >>> 54 Eliptocytes, ovalocytes 98 % 1 Spherocytes 1,8 % >>> 51 Dacryocytes 93 % >>> 16 Acanthocytes 29 % >>> 28 Echinocytes 51 % >>> 51 Schistocytes (and other fragmentocytes) 93 % 3 Polychromasia 5,5 % >>> 26 Hypochromia 47 % 4 Basophilic stippling 7,3 % 1 Rouleaux formation 1,8 % 9 Anisochromia 16 % 1 Knizocytes 1,8 % Platelets - morphology >>> 48 Large platelets 87 % 1 Small platelets 1,8 % >>> 52 Anisocytosis 95 % >>> 52 Platelets hypogranulation 95 % > 18 Megakaryocytic nucleus fragments 33 % Photo 3 Object no.1WBC and other nucleous elements - types of cells 1 Blast 1,8 % >>> 54 Lymphocyte 98 % WBC - morphology 1 Atypical/reactive monocytes 1,8 % > 28 Vacuolisation 51 % 3 LGL/big lymphocytes 5,5 % >>> 36 Lymphocytes - reactive forms 65 % 5 Lymphocytes - atypical forms 9,1 % Object no.2WBC and other nucleous elements - types of cells >>> 55 Neutrophil segment 100 % WBC - morphology >>> 39 No changes 71 % 13 Hypergranulation/toxic granulation 24 % 1 Hypersegmented granulocytes 1,8 % 1 Döhle bodies 1,8 % 1 Nucleus fragments of neutrophiles 1,8 % RBC - morphology 7 Normocytosis 13 % 3 Microcytosis 5,5 % >>> 13 Anisocytosis 24 % 12 Poikilocytosis 22 % >>> 48 Eliptocytes, ovalocytes 87 % >>> 43 Stomatocytes 78 % 13 Dacryocytes 24 % >>> 12 Acanthocytes 22 % > 35 Echinocytes 64 % >>> 35 Target cells 64 % >>> 30 Schistocytes (and other fragmentocytes) 55 % >>> 49 Hypochromia 89 % 2 Howell-Jolly bodies 3,6 % 4 Pappenheimer bodies 7,3 % > 33 Knizocytes 60 % Platelets - morphology >>> 43 No changes 78 % 2 Small platelets 3,6 % 5 Anisocytosis 9,1 % 7 Platelets hypogranulation 13 % Photo 4 Object no.1WBC and other nucleous elements - types of cells >>> 55 Bare nuclei, smudge cell 100 % WBC - morphology 9 No changes 16 % 1 Hypogranulation/agranulation 1,8 % 2 Nucleus fragments of neutrophiles 3,6 % Object no.2WBC and other nucleous elements - types of cells >>> 55 Neutrophil segment 100 % WBC - morphology 6 No changes 11 % >>> 47 Hypogranulation/agranulation 85 % 4 Hypersegmented granulocytes 7,3 % 2 Vacuolisation 3,6 % Object no.3WBC and other nucleous elements - types of cells >>> 55 Monocyte 100 % WBC - morphology >>> 49 No changes 89 % Printed 10.10.2017, summary evaluation of the results should be found at http://www.sekk.cz Page: 2 SEKK NF2/17 - Peripheral Blood Smears - Photos Photo 4 Object no.3WBC - morphology 1 Atypical/reactive monocytes 1,8 % 4 Vacuolisation 7,3 % Object no.4WBC and other nucleous elements - types of cells 1 Neutrophil bar 1,8 % >>> 54 Neutrophil segment 98 % WBC - morphology 8 No changes 15 % >>> 46 Hypogranulation/agranulation 84 % 1 Hyposegmentation or pseudo Pelger anomaly 1,8 % 1 Nucleus fragments of neutrophiles 1,8 % RBC - morphology 3 Microcytosis 5,5 % 14 Macrocytosis 25 % >>> 53 Anisocytosis 96 % 12 Poikilocytosis 22 % >>> 32 Spherocytes 58 % >>> 53 Stomatocytes 96 % 2 Drepanocytes 3,6 % 1 Acanthocytes 1,8 % 7 Target cells 13 % >>> 55 Schistocytes (and other fragmentocytes) 100 % >>> 50 Polychromasia 91 % 2 Hypochromia 3,6 % 2 Basophilic stippling 3,6 % 4 Howell-Jolly bodies 7,3 % 1 Cabot rings 1,8 % 1 Malarial plasmodias 1,8 % >>> 40 Pappenheimer bodies 73 % 5 Anisochromia 9,1 % >>> 46 Knizocytes 84 % Platelets - morphology 41 No changes 75 % 2 Anisocytosis 3,6 % >>> 16 Platelets hypogranulation 29 % Evaluation of the results - scoring system NF2/17 Photo 1 Photo 2 Photo 3 Photo 4 Maximal achievable score: 60 70 60 75 Successful participants (success 60 % and more): 50 (it is 91 %) 45 (it is 82 %) 39 (it is 71 %) 51 (it is 93 %) Minimal success in this round: 46,7 % 45,7 % 35,0 % 52,0 % Maximal success in this round: 98,3 % 100,0 % 100,0 % 100,0 % Number of participants: 55 in all 4 photos: 33 (it is 60 %) in 3 photos: 12 (it is 22 %) Number of participants that succeeded: in 2 photos: 8 (it is 15 %) in 1 photo: 1 (it is 2 %) in no photo: 1 (it is 2 %) Printed 10.10.2017, summary evaluation of the results should be found at http://www.sekk.cz Page: 3.