ACOT8 Antibody Cat. No.: 63-804 ACOT8 Antibody Formalin-fixed and paraffin-embedded human Prostate Flow cytometric analysis of MDA-MB435 cells (bottom carcinoma reacted with ACOT8 Antibody , which was histogram) compared to a negative control cell (top peroxidase-conjugated to the secondary antibody, histogram).FITC-conjugated goat-anti-rabbit secondary followed by DAB staining. antibodies were used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse This ACOT8 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 290-319 amino acids from the C-terminal region of human ACOT8. TESTED APPLICATIONS: Flow, IHC-P, WB September 27, 2021 1 https://www.prosci-inc.com/acot8-antibody-63-804.html For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 36 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: ACOT8 Acyl-coenzyme A thioesterase 8, Acyl-CoA thioesterase 8, Choloyl-coenzyme A thioesterase, HIV-Nef-associated acyl-CoA thioesterase, PTE-2, Peroxisomal acyl-coenzyme ALTERNATE NAMES: A thioester hydrolase 1, PTE-1, Peroxisomal long-chain acyl-CoA thioesterase 1, Thioesterase II, hACTE-III, hACTEIII, hTE, ACOT8, ACTEIII, PTE1, PTE2 ACCESSION NO.: O14734 GENE ID: 10005 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate BACKGROUND: intracellular levels of acyl-CoAs, free fatty acids and CoASH. It may mediate Nef-induced down-regulation of CD4. It may be involved in the metabolic regulation of peroxisome proliferation. September 27, 2021 2 https://www.prosci-inc.com/acot8-antibody-63-804.html REFERENCES: 1) Choudhary C., et.al., Science 325:834-840(2009). 2) Daub H., et.al., Mol. Cell 31:438-448(2008). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 27, 2021 3 https://www.prosci-inc.com/acot8-antibody-63-804.html.