Historical Textile Dyeing with Genista Tinctoria L.: a Comprehensive Study by UPLC-MS/MS Analysis† Cite This: Anal

Historical Textile Dyeing with Genista Tinctoria L.: a Comprehensive Study by UPLC-MS/MS Analysis† Cite This: Anal

Analytical Methods View Article Online PAPER View Journal | View Issue Historical textile dyeing with Genista tinctoria L.: a comprehensive study by UPLC-MS/MS analysis† Cite this: Anal. Methods,2014,6, 8915 Lore G. Troalen,ab Ashley S. Phillips,ac David A. Peggie,ad Perdita E. Barranac and Alison N. Hulme*a Polyphenolic components from Genista species have been well characterised because of their potential as antioxidants and as therapeutic leads; however, the identification of dyer's greenweed (Genista tinctoria L.) in historical textiles has been the subject of only limited studies. This paper presents a comprehensive UPLC-PDA MS/MS study of reference and historical yarns dyed with this species. Several so far Received 25th June 2014 unreported dye components that could assist with the identification of this dye source, were Accepted 22nd July 2014 characterised by MS/MS. Furthermore, the effect of photo-degradation and textile preparation DOI: 10.1039/c4ay01509f techniques (such as over-dyeing) on the dye fingerprint was investigated and the results correlated with www.rsc.org/methods those obtained from historical samples from the Burrell and Bodleian collections, UK. Creative Commons Attribution 3.0 Unported Licence. 1. Introduction components using UPLC-PDA‡ and ESI-MS/MS techniques. To achieve this, an analytical method was developed for a range of Polyphenolic components from Genista species have been well avonoid and isoavonoid dyes, allowing a more efficient characterised because of their potential as antioxidants and as separation of several isomeric dye components from textile therapeutic leads;1,2 however, the identication of dyer's samples. This method was then used to determine the relative greenweed (Genista tinctoria L.) in historical textiles has been amount of dyestuffs present in raw materials, modern yarns and the subject of only limited studies.3,4 Although historically weld historical yarns; examining differences between the plant (Reseda luteola L.) was probably the most widely used European extract and the dye components adsorbed onto textiles, and This article is licensed under a yellow dye plant, it is reported that other dye plants, including relating variations in component ratios to dyeing processes dyer's greenweed (Genista tinctoria L.) and sawwort (Serratula such as over-dyeing, and to the effects of photo-degradation. tinctoria L.), were used as substitutes.5 The avones luteolin and Data from a selection of historical yarns sampled from mid- apigenin, the isoavone genistein, and the glycosides of these sixteenth century English tapestries from the Burrell Collection Open Access Article. Published on 22 Adooleessa 2014. Downloaded 25/09/2021 12:08:51 AM. are known dye components of Genista species.6 But genistein, in Glasgow, UK and the Bodleian Library in Oxford, UK were and its glycosides, are also the main dye components found in then placed in context using the results of this study. other varieties of broom; notwithstanding, the dye source for historical textiles is usually ascribed to dyer's greenweed.7,8 Whilst genistein is the principle component on which this 2. Experimental 4 attribution is made, our studies of the dye extracts of historical 2.1 Materials and chemicals samples show the presence of additional dye components that could enhance this identication. This paper presents a 2.1.1 Plant and textiles. Dyer's broom (Genista tinctoria L.) comprehensive study of the structure of these additional dye from Fibrecra s (George Weil & Sons Ltd.) was used to prepare the reference material. Alum mordanted wool (YW2) and silk (YS3a) yarns were dyed, and over-dyed (YS3b–d), as part of the a EaStCHEM School of Chemistry, Joseph Black Building, Kings Buildings, West Mains Monitoring of Damage to Historic Tapestries project (FP5, EC Road, Edinburgh, EH9 3JJ, UK. E-mail: [email protected] contract number EVK4-CT-2001-00048).9 bNational Museums Scotland, Collections Services Department, 242 West Granton 0 0 Road, Edinburgh, EH5 1JA, UK 2.1.2 Flavone and iso avone standards. Luteolin (3 ,4 ,5,7- 0 cManchester Institute of Biotechnology and School of Chemistry, The University of tetrahydroxyavone), genistein (4 ,5,7-trihydroxyisoavone), 0 0 Manchester, 131 Princess Street, Manchester M1 7DN, UK apigenin (4 ,5,7-trihydroxyavone), prunetin (4 ,5-dihydroxy-7- dNational Gallery London, Scientic Department, Trafalgar Square, London WC2N methoxyisoavone), and biochanin A (5,7-dihydroxy-40- 5DN, UK methoxyisoavone) standards were purchased from Sigma- † Electronic supplementary information (ESI) available: Preparation of an 0 À Aldrich; glycitein (4 ,7-dihydroxy-6-methoxyisoavone) standard authentic sample of isoprunetin; (ESI )MS2 fragmentation of avonoid and À isoavonoid standards at 25 eV; UPLC-PDA and (ESI )MS2 fragmentation of Gt components at 25 eV; UPLC-PDA investigation of references and historical ‡ As commonly adopted by the eld, the abbreviation UPLC has been used for the yarns. See DOI: 10.1039/c4ay01509f technique of ultra-high pressure liquid chromatography (UHPLC). This journal is © The Royal Society of Chemistry 2014 Anal. Methods,2014,6,8915–8923 | 8915 View Article Online Analytical Methods Paper was purchased from Cayman Chemical Company; and chrys- fragmentation patterns. Representative data using a collision oeriol (40,5,7-trihydroxy-30-methoxyavone) and diosmetin energy of 25 eV to illustrate fragmentation patterns is pre- (30,5,7-trihydroxy-40-methoxyavone) standards were purchased sented. Data was collected using Masslynx 4.1 (Waters Corpo- from ExtraSynthese. All commercial standards were >98% ration, Milford, MA, USA) and processed with Origin 8.5 purity. (OriginLab, Northampton, MA, USA). An authentic sample of isoprunetin (40,7-dihydroxy-5- methoxyisoavone) was prepared from genistein by selective 2.3 Plant and yarn extractions acetylation (Ac O, py) to give 7,40-diacetoxy-5-hydroxyiso- 2 2.3.1 Plant extraction. 1.5 g of dried leaves of dyer's avone,10 methylation of the remaining free hydroxyl greenweed were extracted in 100 mL (MeOH : H O 1 : 1, v/v) in [(MeO) SO K CO , acetone] under high dilution conditions11 to 2 2 2 2 3 an ultrasonic bath for 2 hours at 40 C. A fraction of the extract give 7,40-diacetoxy-5-methoxyisoavone, followed by hydrolysis was centrifuged for 10 min at 10 000 rpm and ltered using a (NaHCO aq., MeOH : THF)10 of the acetate groups which 3 PTFE Phenomenex syringe lter (0.2 mm, 4 mm) for UPLC-PDA allowed isolation of isoprunetin in >95% purity aer chroma- analysis. A second fraction of the extract was subjected to the tography (see ESI 1†).12 hydrolysis procedure described below for yarn analysis and 2.1.3 Solutions of standards. The UPLC® system was cali- again analysed by UPLC-PDA. brated using stock solutions of avonoid and isoavonoid 2.3.2 Yarn extraction. The yarns (0.1–0.5 mg) were extrac- standards: (1) a solution containing luteolin and genistein (1.00 m Æ ted with 37% HCl : H2O : MeOH [200 L, 2 : 1 : 1 (v/v/v)], at 100 0.01 mg of each standard) in H2O : MeOH [25 mL, 1 : 1 (v/v); 13 À C for 10 min. Aer ambient cooling to room temperature, the equivalent to 40 mgmL 1]; (2) a solution containing apigenin, Æ extract was centrifuged for 10 min at 10 000 rpm and then chrysoeriol (0.20 0.01 mg of each standard) in H2O : MeOH À ltered directly into Waters UPLC vials® (residual volume of 9 [10 mL, 1 : 1 (v/v); equivalent to 20 mgmL 1]; (3) a solution of mL) using a PTFE Phenomenex syringe lter (0.2 mm, 4 mm). The diosmetin (1.00 Æ 0.01 mg) in H2O : MeOH [25 mL, 1 : 1 (v/v); m À1 extract was then cooled with liquid nitrogen and dried under Creative Commons Attribution 3.0 Unported Licence. equivalent to 40 gmL ]. Diluted solutions were then prepared vacuum using a freeze drier system. The dry residue was then with components at concentrations of 20, 10, 5, 1, 0.5, 0.1, 0.05, À reconstituted with H O : MeOH [40 mL, 1 : 1 (v/v)] – allowing a 0.02 and 0.01 mgmL 1, by dilution with H O : MeOH [1 : 1 (v/v)] 2 2 single injection of 10 mL. using calibrated micro-pipettes. 2.4 Accelerated light-ageing system 2.2 UPLC-PDA and ESI MS systems Accelerated light-ageing experiments were performed at 2.2.1 UPLC-PDA. The UPLC chromatographic method was National Museums Scotland using a Complete Lighting Systems developed using a Waters Acquity UPLC® system with sample light box (St Albans, UK), working at ambient temperature and This article is licensed under a detection using a Waters PDA detector (250 to 500 nm). Data relative humidity (RH). These levels were monitored hourly, were collected by Waters Empower 2 soware and processed together with the intensity of illuminance and UV levels using with Origin 8.5 (OriginLab, Northampton, MA, USA). Sample an Elsec IRLOG environmental Logger and the data periodically extracts were automatically injected via a Rheodyne injector downloaded to computer. Wool yarns dyed with weld (Reseda Open Access Article. Published on 22 Adooleessa 2014. Downloaded 25/09/2021 12:08:51 AM. with a 10 mL sample loop. The bandwidth (resolution) was 1.2 luteola L.) and others with the single dye component genistein nm with a sampling rate of 5 points per s. The method used a were removed over a period of 4571 hours and 1 mg of yarn was PST BEH C18 reverse phase column, 1.7 mm particle size, 150  subsequently hydrolysed following the procedure described 2.1 mm (length  i.d.), set-up with inline lter. The total run above and investigated by HPLC analysis following the method À time was 37.33 min at a ow rate of 250 mL min 1 and the used by Peggie et al.

View Full Text

Details

  • File Type
    pdf
  • Upload Time
    -
  • Content Languages
    English
  • Upload User
    Anonymous/Not logged-in
  • File Pages
    9 Page
  • File Size
    -

Download

Channel Download Status
Express Download Enable

Copyright

We respect the copyrights and intellectual property rights of all users. All uploaded documents are either original works of the uploader or authorized works of the rightful owners.

  • Not to be reproduced or distributed without explicit permission.
  • Not used for commercial purposes outside of approved use cases.
  • Not used to infringe on the rights of the original creators.
  • If you believe any content infringes your copyright, please contact us immediately.

Support

For help with questions, suggestions, or problems, please contact us