Cover Photo by Adi Kehn DEVELOPMENTAL BIOLOGY OF THE SEA URCHIN XXIV April 5-9, 2017 Marine Biological Laboratory Woods Hole, MA Organizers: Athula Wikramanayake Billie Swalla Thierry Lepage Amro Hamdoun The organizers would like to acknowledge the generous support of these sponsors: The organizers would like to thank MRD for providing the T-Shirts Developmental Biology of the Sea Urchin XXIV, The Marine Biological Laboratory, Woods Hole, MA April 5-9, 2017 Wednesday, April 5 Arrival and check in Swope Building: 12:00 PM onwards Dinner 6:00-7:30 PM Opening Plenary Session: From egg to embryo: Developmental and evolutionary insights 7:30-9:10 PM Lillie Auditorium Chair: Athula Wikramanayake (University of Miami) 7:30-7:40 PM-Introductory comments 7:40-8:10 PM-Athula Wikramanayake (University of Miami) “Evolution of the primary egg axis and the emergence of complex metazoans” 8:10-8:40 PM-Uli Technau (University of Vienna) “Cnidarians and the evolution of bilaterality” 8:40-9:10 PM-Yi-Hsien Su (Academia Sinica, Taiwan) “The roles of the maternal and zygotic hypoxia-inducible factor a in sea urchin development” Mixer and posters 9:15-11:00 PM Meigs Room Thursday, April 6 Breakfast 7:00-8:30 AM Plenary Session II: Cell biology of the early embryo 8:30-12:00 AM Lillie Auditorium Chair: Zak Swartz (Whitehead-MIT) 8:30-9:00 AM- Zak Swartz (Whitehead-MIT) “Ensuring transmission of the centromere through meiosis and development” 9:00-9:30 AM- John Henson (Dickinson College) “New insights into the organization of the contractile ring in dividing sea urchin embryos” 9:30-10:00 AM- David Burgess (Boston College) “Role of the Sperm Aster in Nuclear Centering and Determinant Localization” 10:00-10:30 AM-Coffee break 10:30-11:00 AM-Mamiko Yajima (Brown University) “Molecular modification of the G-protein regulator, AGS, contributes to asymmetric cell division in sea urchins” 11:00-11:30 AM- Silvia Sepulveda-Ramirez (New Mexico State University) “Role of cell polarity in morphogenesis ” 11:30-12:00 PM-Tony De Tomaso (UC Santa Barbara) “Dissecting mechanotransduction in real time: How do cells respond to changes in their physical environment” Lunch 12:00-1:30 PM Plenary Session III: Specifying primordial germ cells and stem cells 1:30-3:00 PM Lillie Auditorium Chair: Nathalie Ouhlen (Brown University) 1:30-2:00 PM-Nathalie Ouhlen (Brown University) “Nanos is super important and very cool” 2:00-2:30 PM-William Browne (University of Miami) “Ancient functions in new contexts: the role of Krüppel-like factor genes in the ctenophore Mnemiopsis” 2:30-3:00 PM-Paola Oliveri (University College London) “Development and regeneration in the brittle star A. filiformis” Coffee Break 3:00-3:30 PM, Lillie Auditorium Concurrent Session 1: Cell Biology and Development 3:30-5:20 PM Lillie Auditorium Session Chair: Charles Shuster (New Mexico State University) 3:30-4:00 PM-Charles Shuster (New Mexico State University) “Evolving roles of Arp2/3 actin networks over the course of early development” 4:00-4:20 PM- Wei Wu (University of Miami) “Casein Kinase 1 delta/epsilon mediates anterior-posterior axis formation in the sea urchin embryo, potentially through localized activation of Disheveled” 4:20-4:40 PM- Daphne Schatzberg (Boston University) “V-type H+ ATPase activity is required for dorsal-ventral symmetry breaking in sea urchin embryos” 4:40-5:00 PM- Maria Dolores Molina Jimenez (Institut Biologie Valrose, Nice) “p38 MAPK as an essential regulator of D/V axis specification and skeletogenesis during sea urchin development: a re-evaluation” 5:00-5:20 PM- Margherita Perillo (Boston College) “Characterization of ciliary band neurons expressing pancreatic transcriptional factors” Concurrent Session 2: Primordial germ cells and regeneration 3:30-5:20 PM Speck Auditorium Session Chair: Mariko Kondo (MIBS, University of Tokyo) 3:30-4:00 PM- Mariko Kondo (MIBS, University of Tokyo) “Regeneration studies in two echinoderms, feather star and sea cucumber” 4:00-4:20 PM- Raymond Allen (Duke University) “Cell reprogramming in the urchin embryo after PMC removal and embryo bisection” 4:20-4:40 PM- Tara Fresques (Brown University) “Identifying embryonic mechanisms that induce a germ cell fate in sea stars” 4:40-5:00 PM- Minyan Zheng (Carnegie Mellon University) “Characterization of apical pole domain associated gene regulatory networks during sea star larval regeneration” 5:00-5:20 PM- Andrew Wolf (Carnegie Mellon University) “A shift from normal to regeneration-specific proliferation in the sea star Patiria miniata” Dinner 5:45-7:30 PM Swope Plenary Session IV: Making neurons and sensory organs 7:30-9:00 PM Lillie Auditorium Session Chair: Ryan Range (Mississippi State University) 7:30-8:00 PM-Ryan Range (Mississippi State University) “The emerging Wnt signaling network that governs anterior-posterior neuroectoderm patterning in the sea urchin embryo” 8:00-8:30 PM-Robert Burke (University of Victoria) “Patterning Neurogenesis” 8:30-9:00 PM-Roberto Feuda (California Institute of Technology) “Eyes without the master: photoreceptor specification in the sea urchin larva is independent of the retinal determination network” Posters and mixer 9:00-11:00 PM Meigs Room Friday, April 7 Breakfast 7:00-8:30 AM Plenary Session V: Cellular and molecular mechanisms underlying specification and morphogenesis 8:30-12:00 PM Lillie Auditorium Chair: Ina Arnone (Stazione Zoologica) 8:30-9:00 AM- Dede Lyons (Scripps Institute of Oceanography) “Morphogenesis along the animal-vegetal axis:fates of primary quartet micromere daughters in the gastropod Crepidula fornicata” 9:00-9:30 AM-Ina Arnone (Stazione Zoologica) “Echinoderm GRNs are going omics: insight into the evolution of gut patterning” 9:30-10:00 AM- David McClay (Duke University) “GRNs regulating morphogenesis” 10:00-10:30 AM Coffee Break 10:30-11:00 AM-Elaine Seaver (UF-Whitney Marine Laboratory) “Establishment of body axes in the annelid Capitella teleta” 11:00-11:30 AM-Isabel Peter (California Institute of Technology) “Assessing the relationship between structure and function in developmental circuits” (California Institute of Technology) 11:30-12:00 PM-Shunsuke Yaguchi (University of Tsukuba) “Troponin system in the sea urchin embryos” Lunch 12:00-1:30 PM Plenary Session VI: Patterning the ectoderm 1:30-3:00 PM Lillie Auditorium Chair: Cyndi Bradham (Boston University) 1:30-2:00 PM-Thierry Lepage (Institut Biologie Valrose, Nice) “The ETS domain YanTel as a key maternal regulator of axis formation at the crossroads of Wnt and Nodal signaling in the sea urchin embryo” 2:00-2:30 PM-Cyndi Bradham (Boston University) “New Cues for Skeletal Patterning in Sea Urchin Embryos” 2:30-3:00 PM-Smadar ben Tabou de Leon (University of Haifa) “VEGF activates a complex gene network with angiogenetic motifs to control sea urchin spiculogenesis” Coffee Break 3:00-3:30 PM, Lillie Auditorium Concurrent Session 3: Morphogenesis 3:30-5:20 PM Lillie Auditorium Session Chair: Jose Espinoza (Scripps Institute of Oceanography) 3:30-4:00 PM – Derk Joester (Northwestern University) “Title TBA” 4:00-4:20 PM- Claudia Cuomo (Statione Zoologica) “SpMeis: a “novel” key factor shaping the gut of the sea urchin post- gastrula embryo 4:20-4:40 PM- Jose Espinosa (Scripps Institute of Oceanography) “Small micromere migration and a possible role for lysophosphatidic acid signaling in left/right asymmetry” 4:40-5:00 PM- Andrew George (Duke University) “Characterizing Epithelial to mesenchymal transition of pigment cells in the sea urchin Lytechinus variegatus” 5:00-5:20 PM- Lama Khalaily (University of Haifa) “Upstream regulation of sea urchin skeletal patterning and VEGF signaling” Concurrent Session 4: GRNs in development and evolution 3:30-5:40 PM Speck Auditorium Session Chair: Greg Cary (Carnegie Mellon University) 3:30-4:00 PM- Greg Cary (Carnegie Mellon University) “Evolution of the Delta-HesC feedback mechanism driving mesodermal subtype specification in echinoderms” 4:00-4:20 PM- Leslie Slota (Duke University) “Three populations of neurons in the sea urchin develop using gene regulatory networks highly conserved with vertebrates” 4:20-4:40 PM- Jon Valencia (California Institute of Technology) “The regulatory states in the sea urchin larva” 4:40-5:00 PM- Marina Martinez-Bartolome (Mississippi State University) “Wnt16-Fzl1/2/7-NFAT signaling antagonizes Wnt1/Wnt8-Fzl5/8-JNK signaling mediated restriction of the anterior neuroectoderm along the anterior-posterior axis in the sea urchin embryo” 5:00-5:20 PM-Catherine Guay (Rutgers University) “Testing the utility of sea urchin embryos to discover human embryonic cis-regulatory modules” Dinner 5:45-7:30 PM Plenary Session VII: Genomic and developmental evolution 7:30- 9:00 PM Lillie Auditorium Chair: Veronica Hinman (Carnegie Mellon University) 7:30-8:00 PM-Veronica Hinman (Carnegie Mellon University) “Regeneration of larval P. miniata” 8:00-8:30 PM-Billie Swalla (University of Washington) “Evolution, development and regeneration in hemichordates” 8:30-9:00 PM-Greg Wray (Duke University) “Rapid evolution of key GRN interactions within the sea urchin genus Heliocidaris” Posters and mixer 9:00-11:00 PM Meigs Room Saturday, April 8 Breakfast 7:00-8:30 AM Plenary Session VIII: Environmental and epigenetic influences on development 8:30-12:00 PM Lillie Auditorium Chair: Meike Stumpp (Christian-Albrechts-University Kiel) 8:30-9:00 AM- Meike Stumpp (Christian-Albrechts-University Kiel) “The alkaline larval gut – a key trait under environmental change?” 9:00-9:30 AM-Jonathan Rast (University of Toronto) “The gene regulatory