Revista Mexicana de Ingeniería Química ISSN: 1665-2738 [email protected] Universidad Autónoma Metropolitana Unidad Iztapalapa México Reyes-Méndez, A.I.; Figueroa-Hernández, C.; Melgar-Lalanne, G.; Hernández-Sánchez, H.; Dávila-Ortiz, G.; Jiménez-Martínez, C. PRODUCTION OF CALCIUM- AND IRON-BINDING PEPTIDES BY PROBIOTIC STRAINS OF Bacillus subtilis, B. clausii AND B. coagulans GBI-30 Revista Mexicana de Ingeniería Química, vol. 14, núm. 1, 2015, pp. 1-9 Universidad Autónoma Metropolitana Unidad Iztapalapa Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=62037106001 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Revista Mexicana deIngenier´ıaQu´ımica Academia Mexicana de Investigaci´ony Docencia en Ingenier´ıaQu´ımica, A.C. Revista Mexicana de Ingeniería Química Volumen 14, N´umero1, Abril 2015 CONTENIDO ISSN 1665-2738 Vol. 14, No. 1 (2015) 1-9 1 Volumen 8, número 3, 2009 / Volume 8, number 3, 2009 PRODUCTION OF CALCIUM- AND IRON-BINDING PEPTIDES BY PROBIOTIC STRAINS OF Bacillus subtilis, B. clausii AND B. coagulans GBI-30 1 213 Derivation and application of the Stefan-Maxwell equations PRODUCCION´ DE PEPTIDOS´ FIJADORES DE CALCIO Y HIERRO POR CEPAS PROBIOTICAS´ (Desarrollo DE y aplicaciónBacillus de las ecuaciones subtilis de Stefan-Maxwell), B. clausii Y B. coagulans GBI-30 Stephen Whitaker A.I. Reyes-Mendez,´ C. Figueroa-Hern andez,´ G. Melgar-Lalanne, H. Hernandez-S´ anchez´ ∗, G. Davila-Ortiz,´ and C. Biotecnología / Biotechnology Jimenez-Mart´ ´ınez Departamento de Graduados e Investigaci´onen Alimentos, Escuela Nacional de Ciencias Biol´ogicas,Instituto 245 Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo Polit´ecnicoNacional, Carpio y Plan de Ayala, Col. Sto. Tom´as,CP 11340, M´exico,D.F. M´exico. intemperizados en suelos y sedimentos Recibido 4 de Noviembre 2013; Aceptado 24 de Enero de 2014 (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments) Abstract S.A. Medina-Moreno, S. Huerta-Ochoa, C.A. Lucho-Constantino, L. Aguilera-Vázquez, A. Jiménez- Some strains of Bacillus subtilisGonzález, yB. M. clausiiGutiérrez-Rojasand B. coagulans are commercially used as probiotic bacteria and their proteolytic activity is well259 Crecimiento, known. The sobrevivencia aim of thisy adaptación work de was Bifidobacterium to evaluate infantis their a capacitycondiciones to ácidas produce calcium- and iron- binding peptides when grown in culture media with different nitrogen sources. The fermentation media included broths containing tryptic soy (TS), (Growth, casein survival peptone and (CP), adaptation soy of peptone Bifidobacterium (SP), andinfantis a crudeto acidic phycobiliprotein conditions) extract (CPE). Cell-free supernatants (CFS) were preparedL. Mayorga-Reyes, from the P. fermented Bustamante-Camilo, media afterA. Gutiérrez-Nava, 24, 48 and E. 72 Barranco-Florido h of fermentation y A. Azaola- and, tested for degree of hydrolysis (DH). Calcium-Espinosa and iron-binding activities were determined as well. When the inocula were prepared in a medium designed for the265 production Statistical approach of alkaline to optimization proteases of (AP),ethanol the fermentation degree ofby hydrolysisSaccharomyces and cerevisiae the mineral-binding in the activity in the CFS were higher. Thepresence best of resultsValfor® forzeolite calcium-binding NaA activity and DH were obtained when B. subtilis and B. coagulans grew in a CPE-containing (Optimización medium. estadística de Analogous la fermentación results etanólica were de foundSaccharomyces for the cerevisiae iron-binding en presencia activity; de nevertheless, this bioactivity was also highzeolita for B.Valfor® clausii zeolitewhen NaA) grown in broths containing TS-, CP- and, SP. Keywords: bioactive peptides,G. Inei-Shizukawa,Bacillus subtilis H. A. Velasco-Bedrán,, Bacillus clausiiG. F. Gutiérrez-López, Bacillus and coagulans H. Hernández-Sánchez, calcium-binding, iron-binding, proteolysis, spirulina. Ingeniería de procesos / Process engineering Resumen 271 Localización de una planta industrial: Revisión crítica y adecuación de los criterios empleados en Algunas cepas de Bacillus subtilis, B. clausii y B. coagulans se utilizan comercialmente como probioticos´ y su actividad esta decisión proteol´ıtica es de sobra conocida. El objetivo de este trabajo fue la evaluacion´ de estos microorganismos debido a su (Plant site selection: Critical review and adequation criteria used in this decision) capacidad para producir peptidos´ fijadores de calcio y hierro cuando son cultivados en medios con diferentes fuentes de J.R. Medina, R.L. Romero y G.A. Pérez nitrogeno.´ Los medios para la fermentacion´ incluyeron caldos con soya y triptona (ST), peptona de case´ına (PC), peptona de soya (PS) y, un extracto crudo de ficobiliprote´ına (ECF). Se obtuvieron sobrenadantes libres de celulas´ (SLC) a partir de los caldos de fermentacion´ y se determino´ el grado de hidrolisis´ (GH) y la bioactividad fijadora para calcio y hierro. El GH y la actividad fijadora de minerales fue mayor cuando se emplearon inoculos´ preparados a partir de un medio disenado˜ para la produccion´ de proteasas alcalinas (AP). El mejor resultado para el GH y la bioactividad fijadora de calcio se obtuvo con B. subtilis y B. coagulans cultivados en caldo con ECF como fuente de nitrogeno.´ Se observaron resultados similares para la actividad fijadora de hierro, sin embargo, tambien´ se obtuvieron buenos resultados con B. clausii cuando se utilizaron para su crecimiento los caldos ST, PC y PS. Palabras clave: peptidos´ bioactivos, Bacillus subtilis, Bacillus clausii, Bacillus coagulans, fijadores de calcio, fijadores de hierro, proteolisis,´ spirulina. ∗Corresponding author. E-mail: : [email protected] Tel. +(52 55) 5729-6000 Publicado por la Academia Mexicana de Investigacion´ y Docencia en Ingenier´ıa Qu´ımica A.C. 1 Reyes-M´endezet al./ Revista Mexicana deIngenier ´ıaQu ´ımica Vol. 14, No. 1 (2015) 1-9 1 Introduction food. Protein content up to 64% (dry basis) can be reached under the right culture conditions (Pandey and The role of proteins as biofunctional compounds Tiwari, 2010). Within the most important proteins, has been studied in several works. Many of these phycobiliproteins are possibly, the components with proteins show their functional activities directly or the highest commercial value. Due to their protein after enzymatic hydrolysis either in vitro or in vivo. nature, unique color, and other properties with a Proteolytic enzymes have many applications in food wide range of promising applications including the science and technology (Salazar-Leyva et al., 2014) production of bioactive peptides (Simeunovic´ et al., and their action provides a rich source of biological 2012). active peptides whose sequences are inactive inside the The genus Bacillus includes moderately precursor protein. They are released through the action thermophilic, aerobic or facultative anaerobic, of proteolytic enzymes from different origins such proteolytic spore-forming rods. Some strains have as microorganisms, digestive system, etc (Korhonen, been catalogued as probiotics and can produce a broad 2009; Korhonen and Philanto, 2006). Starter and non- spectrum of bioactive peptides, with great potential for starter bacteria used in the manufacture of fermented biotechnological and biopharmaceutical applications foods (Mayo et al. 2010; Gonzales-Olivares´ et (Cutting, 2011; van Dijl et al., 2013, Seyedeh et al., al., 2011; Choi et al., 2012; Ram´ırez-Romero et 2007). The aim of this study was the evaluation of al., 2013) can thus, generate bioactive peptides. the capacity of three probiotic strains of Bacillus to Bioactivities such as mineral-binding have a positive produce calcium- and iron-binding peptides when impact on human health. The deficiency in the grown in culture media with different nitrogen sources, intake of some minerals like calcium and iron is a crude extract of phycobiliproteins included. Two a worldwide health problem. Iron deficiency is different growth media were used to explore their estimated to affect about 30% of the world population effect on the production of proteases: the first was (Figueroa et al., 2012). Iron supplementation in an alkaline media for optimal growth (AM) and the the form of tablets and food fortification has not second, a medium to activate proteases (AP). been successful in developing countries, and iron deficiency is still the most important deficiency related to malnutrition. Dietary factors have been shown 2 Materials and methods ff that a ect iron intake and some milk-derived peptides, 2.1 Microorganisms in particular caseinophosphopeptides (CPP) produced during enzymatic digestion enhance iron absorption The Bacillus strains were isolated from commercial (Jung et al., 2007; Bouhallab et al. 2002). Calcium probiotic-containing products: Bacillus coagulans is an essential mineral in the human body since GBI-30 from Digestive Advantage®, Bacillus clausii it regulates many cellular processes such as nerve from Enterogermina®, and Bacillus subtilis from response, bone growth, muscle contraction, cardiac Salvacolon®. Spore germination was induced by a functions and many more (Sussman et al., 1989; Bass heat treatment at 70°C for 30 min (Vepachedu