Collaborative Project (large-scale integrating project) Grant Agreement 226273 Theme 6: Environment (including Climate Change) st th Duration: March 1 , 2009 – February 29 , 2012 Deliverable D3.3-2: The importance of invertebrate spatial and temporal variation for ecological status classification for European lakes Lead contractor: Sapienza University of Rome (UNIROMA) Contributors: Angelo Solimini, Marcello Bazzanti, Luciana Mastrantuono, Francesca Pilotto (UNIROMA), Helen Michels, Piet Verdonschot (ALTERRA), Eleine McGoff, Leonard Sandin (SLU), Gwendolin Porst, Steffen Bader, Elise Münch, Martin Pusch (IGB), Mike Dunbar (CEH), Ralph Clarke (BOURNEU) Due date of deliverable: Month 24 Actual submission date: Month 26 Project co-funded by the European Commission within the Seventh Framework Programme (2007- 2013) Dissemination Level PU Public X PP Restricted to other programme participants (including the Commission Services) RE Restricted to a group specified by the consortium (including the Commission Services) CO Confidential, only for members of the consortium (including the Commission Services) Deliverable D3.3-2: variability of lakes invertebrates Content Non-technical summary ............................................................................................................. 5 Chapter 1. Introduction and aim of the deliverable.................................................................... 6 1.1 Why we need to assess invertebrate spatial and temporal variation in lakes................... 6 1.2 Brief description of case studies....................................................................................... 7 Chapter 2. Time-for Space analysis on a long term data series of macroinvertebrates of the Naardermeer............................................................................................................................... 9 2.1 Introduction ................................................................................................................ 9 2.2 Methods.................................................................................................................... 10 Study area......................................................................................................................... 10 Data collection.................................................................................................................. 11 Data analysis .................................................................................................................... 11 Statistics ........................................................................................................................... 12 2.3 Results ...................................................................................................................... 13 Analysis Multimetric MM dataset 1981 to 2004 ............................................................. 14 Analysis % EPT Abundance dataset 1981 to 2004.......................................................... 14 Analysis Multimetric MM dataset 1992 to 2004 ............................................................. 16 Analysis %EPT abundance dataset 1992 to 2004 ............................................................ 16 2.4 Discussion and Conclusion ............................................................................................ 17 Analysis dataset 1981-2004 ............................................................................................. 17 Analysis dataset 1992-2004 ............................................................................................. 17 2.5 References ...................................................................................................................... 18 Chapter 3. Comparisons of spatial variability of macrozoobenthos between sublittoral and profundal zones in subAlpine lakes ......................................................................................... 19 3.1 Introduction .............................................................................................................. 19 3.2 Methods.......................................................................................................................... 20 Study area, and macroinvertebrate sampling ................................................................... 20 Environmental variables................................................................................................... 21 Data analysis .................................................................................................................... 22 3.3 Results ............................................................................................................................ 23 Spatial component:........................................................................................................... 24 2 Deliverable D3.3-2: variability of lakes invertebrates Variance partitioning:....................................................................................................... 24 3.4 Discussion and Conclusion ............................................................................................ 27 Spatial and enviornmental variables ................................................................................ 28 Variance partitioning:....................................................................................................... 29 3.5 References ...................................................................................................................... 30 Chapter 4. Analysis of invertebrate diversity metrics sensitive to eutrophication in different depth zones of natural Mediterranean lakes............................................................................. 32 4.1 Introduction .............................................................................................................. 32 4.2 Methods.......................................................................................................................... 33 Study area and environmental features of lakes............................................................... 33 Characteristics of the macroinvertebrate dataset.............................................................. 35 Data analysis and selection of metrics ............................................................................. 35 4.3 Results ............................................................................................................................ 37 Relationships among macroinvertebrate taxa, depth zones and trophic levels of lakes . 37 Testing of selected metrics based on macroinvertebrates ................................................ 39 4.4 Discussion and Conclusion ............................................................................................ 40 4.5 References ...................................................................................................................... 45 Chapter 5. Effect of catchment land use on littoral macroinvertebrate response to local habitat structure and trophic state ........................................................................................................ 50 5.1 Introduction .............................................................................................................. 50 5.2 Methods.......................................................................................................................... 52 Data collection and taxonomic resolution........................................................................ 52 Sample sites...................................................................................................................... 52 Habitat descriptors and chemical analysis ....................................................................... 53 Statistics ........................................................................................................................... 53 5.3 Results ............................................................................................................................ 55 Land use effects on the environmental variables ............................................................. 55 Variance partitioning........................................................................................................ 56 Substrate........................................................................................................................... 59 5.4 Discussion ...................................................................................................................... 61 Catchment land use differences........................................................................................ 61 3 Deliverable D3.3-2: variability of lakes invertebrates Substrate and nutrient effects on macroinvertebrates ...................................................... 62 Substrate stratification...................................................................................................... 63 Management implications ................................................................................................ 64 5.5 Conclusion...................................................................................................................... 65 5.6 References ...................................................................................................................... 65 Appendix .................................................................................................................................