|HAI LUI AUN ACUTUS010070601B2 UN EN KOMMA IN WHITE (12 ) United States Patent (10 ) Patent No. : US 10 ,070 ,601 B2 Olivier et al. (45 ) Date of Patent : Sep . 11, 2018 ( 54 ) IDENTIFICATION AND THE USE OF KRP 9 , 062 ,323 B2 6 / 2015 Olivier et al. 9 ,556 ,448 B2 * 1 /2017 Olivier .. .. .. .. .. .. CO7K 14 /415 MUTANTS IN PLANTS 9 , 637, 754 B2 5 / 2017 Olivier et al . 2004 /0019926 A1 1 / 2004 Frankard et al. (71 ) Applicant : Targeted Growth , Inc. , Seattle , WA 2007 /0044171 Al 2 / 2007 Kovalic et al . (US ) 2007 /0056058 A1 * 3 / 200 .. CO7K 14 /415 800 / 290 ( 72 ) Inventors : Jean Paul Olivier , Seattle , WA (US ) ; 2008 / 0134355 A1 6 / 2008 Van Camp Dayna L . Loeffler , Seattle , WA (US ) 2008 /0216193 A19 /2008 Savidge et al. 2008 / 0307546 A1 12 / 2008 Veylder et al. 2009 /0070894 A1 * 3 /2009 Frankard .. .. .. CO7K 14 /415 ( 73) Assignee : Targeted Growth , Inc. , Seattle , WA 800 / 278 (US ) 2009 /0087878 A9 4 /2009 La Rosa et al. 2009 / 0144863 A1 6 / 2009 Song et al . ( * ) Notice : Subject to any disclaimer, the term of this 2011/ 0135647 A1 6 / 2011 Nakamura et al. patent is extended or adjusted under 35 2012 / 0131698 A1 5 / 2012 Olivier et al. 2012 / 0284813 Al 11/ 2012 Olivier et al. U . S . C . 154 (b ) by 0 days . 2014 / 0143900 A1 5 / 2014 Olivier et al . 2014 /0331362 AL 11 /2014 Olivier et al. (21 ) Appl . No. : 15 /365 , 544 2016 / 0002656 A1 1 / 2016 Olivier et al. ( 22 ) Filed : Nov . 30 , 2016 2017 /0183680 A16 / 2017 Olivier et al. FOREIGN PATENT DOCUMENTS (65 ) Prior Publication Data (65 ) WO WO 1999 / 064599 AL 12 / 1999 US 2017 /0142919 A1 May 25, 2017 WO WO 2000 / 060087 A2 10 / 2000 WO WO 2002 /081623 A2 10 / 2002 Related U . S . Application Data WO WO 2005 / 007829 A2 1 / 2005 WO WO 2005 /024029 A2 3 / 2005 (63 ) Continuation of application No . 14 / 111, 292 , filed as Wo WO 2006 / 081029 A2 8 / 2006 application No. PCT/ US2012 /033047 on Apr. 11 , WO WO 2007 /016319 A2 2 / 2007 WO WO 2009 /092009 A2 7 / 2009 2012 , now Pat . No . 9 , 556 ,448 . wo WO 2010 /099083 Al 9 / 2010 (60 ) Provisional application No . 61 /474 ,201 , filed on Apr. WO WO 2012 / 065166 A2 5 / 2012 WO WO 2012 / 142106 Al 10 / 2012 11 , 2011 . WO WO 2012 / 142116 A2 10 / 2012 (51 ) Int. CI. A01H 6 /46 ( 2018 .01 ) OTHER PUBLICATIONS C12N 15 /01 ( 2006 .01 ) A01H 1 / 02 ( 2006 .01 ) Cheng et al , 2013 , The Plant J ., 75 : 642 -655 . * A01H 1/ 04 (2006 . 01) (Continued ) (52 ) U . S . CI. ??? . .. A01H 1 /02 ( 2013 .01 ) ; A01H 1/ 04 Primary Examiner - Jason Deveau Rosen (2013 .01 ) ( 74 ) Attorney, Agent, or Firm — Cooley LLP ( 58 ) Field of Classification Search None (57 ) ABSTRACT See application file for complete search history . The invention provides a plant cell, part , tissue culture or whole plant comprising at least one disrupted KRP gene of ( 56 ) References Cited the present invention . The present invention also provides methods of increasing weight, size, and / or number of one or U . S . PATENT DOCUMENTS more organs , and /or yield of a plant by utilizing the dis 5 , 958 , 769 A 9 / 1999 Roberts et al . rupted KRP genes of the present invention . Furthermore , 6 , 087 ,175 A 7 / 2000 John methods of breeding plants to produce new plants having 6 , 114 ,608 A 9 / 2000 Mettler et al. increased weight, size , and /or number of one or more 6 , 559 , 358 B1 5 / 2003 Murray organs , and / or yield are provided . The present invention 6 ,710 , 227 B1 3 / 2004 Inze et al. 7 , 122 ,658 B1 10 / 2006 Lappegard et al. provides isolated Kinase inhibitor Protein (KIP ) Related 7 , 329 ,799 B2 * 2 / 200800 : Savidge . CO7K 14 /415 Protein (KRP ) polynucleotide sequences and isolated KRP 426 / 531 polypeptide sequences and methods of their use . Exemplary 7 , 553, 954 B2 6 / 2009 Andersen et al . plants include wheat, rice and soy bean . 7 , 803 , 990 B2 9 / 2010 Abbitt 7 , 807 , 872 B2 10 / 2010 Frankard et al . 8 ,431 ,775 B24 / 2013 Hegstad et al. 10 Claims, 17 Drawing Sheets 8 , 742 , 205 B26 / 2014 Olivier et al . Specification includes a Sequence Listing . US 10 ,070 ,601 B2 Page 2 References Cited Kwon , T . K . et al. “ Identification of cdk2 binding sites on the p27Kipl ( 56 ) cyclin -dependent kinase inhibitor ” , Oncogene , 16 ( 6 ) :755 - 762 ( 1988 ) . OTHER PUBLICATIONS Leenhardt et al. “ Wheat lipoxygenase activity induces greater loss of carotenoids than vitamin E during breadmaking ” , J Agric Food U . S . Appl. No . 14 /715 ,317 , Notice of Allowance dated Apr. 18 , Chem . , 54 ( 5 ) : 1710 - 1715 (2006 ) . 2017 , 10 pages. 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