9th Adhesion GPCR Workshop Sept. 13–15, 2018 | The Nines Hotel | Portland, OR, USA Organized by Kelly Monk, Ph.D. Professor and Co-Director, Vollum Institute Oregon Health & Science University Day 1 Agenda Thursday, September 13th 9:00-9:10 Opening remarks 9:10-10:30 Session 1: aGPCRs in Development Chairpersons: Simone Prömel Caroline Formstone 9:10-9:30 The enigmatic trans function of the Adhesion GPCR Latrophilin acts non-cell 8 autonomously in fertility Simone Prömel Leipzig University, Leipzig, Germany 9:30-9:50 BAI1/ADGRB1 sculpts the dendritic arbors of hippocampal pyramidal neurons 9 via RhoA-dependent growth restriction Joseph Duman Baylor College of Medicine, Houston, TX 9:50-10:10 Dystroglycan binds Celsr3 (Adgrc3) to regulate commissural axon 10 guidance Kevin Wright Oregon Health & Science University, Portland, OR 10:10-10:30 A role for the Adhesion-GPCR Celsr1 in contact-mediated alignment of cell 11 behaviour Caroline Formstone Kings College London, University of Hertfordshire, Hatfield, UK 10:30-11:00 Coffee Break 11:00-12:40 Session 2: Signaling & Activation I Chairpersons: Ines Liesbscher Randy Hall 11:00-11:20 The physiological role of the mechano-responsive aGPCR GPR133 12 Ines Liebscher Leipzig University, Leipzig, Germany 11:20-11:40 Signaling and trafficking of GPR64/ADGRG2are regulated by its N-terminal 13 fragment and tethered peptide Nariman Balenga University of Maryland School of Medicine, Baltimore, MD 11:40-12:00 Coincidence detection of membrane stretch and extracellular pH by a proton- 14 sensing G protein coupled receptor Maike Glitsch University of Oxford, Oxford, UK 12:00-12:20 Signaling and Regulation of the BAI Sub-Family of Adhesion GPCRs 15 Randy Hall Emory University, Atlanta, GA 12:20-12:40 Lightning Talks Chair: Ryan Gray 12:20-12:25 A Tale of Two aGPCRs (in Sensory Neuron Myelination): ADGRG6 and 16 ADGRG1 Amit Mogha, Oregon Health & Science University, Portland, OR Day 1 Agenda continued Thursday, September 13th 12:25-12:30 G protein-coupled receptor 56 (GPR56) as a potential functional regulator of 17 normal and leukemic human stem cells Heather Duncan McGill University, Montreal, QC, Canada 12:30-12:35 ADGRG6 (Gpr126) regulates EMT of endocardial cells during valve formation 18 Gentian Musa Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany 12:35-12:40 Role of GPR110 in Breast Cancer 19 Meghana Trivedi University of Houston College of Pharmacy, Houston, TX 12:40-14:10 Lunch (provided) 14:10-15:30 Session 3: aGPCRs in Health & Disease Chairpersons: Hsi-Hsien Lin Gabi Aust 14:10-14:30 The Adhesion G protein-coupled receptor G6 is essential for homeostasis of 20 the intervertebral disc in mice Ryan Gray University of Texas at Austin Dell Medical School, Austin, TX 14:30-14:50 Roles of the ADGRA family in glandular development, lineage commitment and 21 tumorigenesis Elena Spina New York University School of Medicine, New York, NY 14:50-15:10 Regulation of canonical WNT7 signaling by GPR124/ADGRA2 22 Mario Vallon Stanford University, Stanford, CA 15:10-15:30 Together or apart, but alwaysclose:Latrophilin-1 mediates axonal attraction 23 induced by proteolytically released Lasso Yuri Ushkaryov University of Kent, Chatham, UK 15:30-15:50 Mechano-dependent phosphorylation of CD97/ADGRE5 at its PDZ-binding motif 24 modulates cellular detachment Gabi Aust Leipzig University, Leipzig, Germany 15:50-16:15 Coffee Break 16:15-17:15 KEYNOTE 25 Ubiquitin and Cell Signaling by Protease-activated Receptors JoAnn Trejo University of California, San Diego, CA Introduction by Randy Hall 17:15-19:15 Poster session – snacks and bar 17:15-18:15 Odd numbered posters present 18:15-19:15 Even numbered posters present 19:15-20:00 Meeting for aGPCR board Ruby Boardroom – see map located in Visitor Information Other attendees free Day 2 Agenda Friday, September 14th 9:00-10:20 Session 4: Structure & Function Chairpersons: Demet Arac Antony Boucard 9:00-9:20 Structural and functional basis of adhesion GPCR activation 26 Demet Arac University of Chicago, Chicago, IL 9:20-9:40 Annotation and quantification of adhesion GPCR splice variants 27 Alexander Knierim Leipzig University, Leipzig, Germany 9:40-10:00 CryoEM visualization of G protein-coupled receptors 28 Georgios Skiniotis Stanford University, Stanford, CA 10:00-10:20 Adhesion G protein-coupled receptors adgrl/latrophilins physically and functionally 29 interact with the actin cytoskeleton Antony Boucard Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico 10:20-11:40 Session 5: Biological Functions I Chairpersons: Felix Engel Kim Tolias 10:20-10:40 ADGRG6 (Gpr126) is a mechano-responsive gene 30 Felix Engel Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany 10:40-11:00 Systematic affinity proteomics identifies functional modules associated with 31 adhesion GPCRs Uwe Wolfrum Johannes Gutenberg University of Mainz, Mainz, Germany 11:00-11:20 The adhesion G protein-coupled receptor GPR97/ADGRG3 is expressed in human 32 granulocytes and triggers antimicrobial effector functions Cheng-Chih (Andy) Hsiao University of Amsterdam, Amsterdam, The Netherlands 11:20-11:40 The adhesion-GPCR bai1 promotes excitatory synaptogenesis by coordinating 33 bidirectional trans-synaptic signaling Kim Tolias Baylor College of Medicine, Houston, TX 11:40-12:00 Community Session Announce 2020 Meeting Location 12:00-14:00 Lunch and extended poster viewing Lunch provided 14:00-15:40 Session 6: Signaling & Activation II Chairpersons: Nicole Scholz Jim Bridges 14:00-14:20 The adhesion-GPCR latrophilin/dCirl shapes the development of the nmj in 34 drosophila Nicole Scholz Rudolf-Schönheimer-Institute of Biochemistry, Leipzig, Germany Day 2 Agenda continued Friday, September 14th 14:20-14:40 The Role of GPR110-dependent signaling in neurodevelopment and neuroprotection 35 Hee-Yong Kim National Institutes of Health, Bethesda, MD 14:40-15:00 GPR126 function is regulated by its extracellular region 36 Katherine Leon University of Chicago, Chicago, IL 15:00-15:20 ADGRB1 suppresses cerebellar transformation by sequestering mdm2 from p53 37 Erwin Van Meir Emory University, Atlanta, GA 15:20-15:40 Molecular Analysis of ADGRF5 Signaling Determinants Required for Alveolar 38 Homeostasis Jim Bridges Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 15:40-16:00 Coffee break 16:00-17:20 Session 7: Biological Functions III Chairpersons: Garret Anderson Benoit Vanhollebeke 16:00-16:20 Role of Latrophilin Adhesion GPCRs in Synaptic Assembly 39 Garret Anderson University of California, Riverside, CA 16:20-16:40 Gpr126 NTF regulates cardiomyocyte depolarization and delamination in zebrafish 40 trabeculation Swati Srivastava Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany 16:40-17:00 Adhesion GPCR: novel targets to modulate glucose homeostasis 41 Doreen Thor Leipzig University, Leipzig, Germany 17:00-17:20 Neurovascular development via Gpr124/Reck-dependent Wnt7/β-catenin signaling 42 Benoit Vanhollebeke Université Libre de Bruxelles, Bruxelles, Belgium 19:00-21:00 Baerlic Brewing Co.– optional. Max and walking directions located in Visitor Information. Day 3 Agenda Saturday, September 15th 9:30-10:30 KEYNOTE 43 Dissecting Neuromodulatory Circuits and Signaling in Affective Behavior Michael Bruchas Washington University, St. Louis, MO Introduction by Kelly Monk 10:40-12:00 Session 8: Signaling & Activation III Chairpersons: Gregary Tall Xianhua Piao 10:40-11:00 Harnessing the AGPCR tethered-peptide-agonist mechanism for high throughput 44 screening of chemical modulators Gregory Tall University of Michigan, Ann Arbor, Michigan 11:00-11:20 Orphan aGPCR GPR110 –a novel potential player in kidney function 45 Sandra Huth Leipzig University, Leipzig, Germany 11:20-11:40 Renal Expression of Adhesion GPCR Gpr116 (ADGRF5) Plays a Role in Urinary 46 Concentration in Mice Nathan Zaidman Johns Hopkins University School of Medicine, Baltimore, MD 11:40-12:00 The adhesion GPCR VLGR1 is a part of focal adhesion complexes, cell migration 47 and mechanotransduction Deva Krupakar Kusuluri Johannes Gutenberg University of Mainz, Mainz, Germany 12:00-12:20 Oligodendrocyte GPR56/ADGRG1 integrates signals from microglia and the 48 extracellular matrix to regulate developmental myelination and myelin repair Xianhua Piao Children’s Hospital and Harvard Medical School, Boston, MA 12:20-12:30 Closing remarks .