Appendix: Supplementary tables and raw data Evolutionary genetic analysis of an invasive population of sculpins in the Lower Rhine I n a u g u r a l - D i s s e r t a t i o n zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Universität zu Köln vorgelegt von Arne W. Nolte aus Oldenburg Köln, 2005 Data formats and access: According to the guidelines of the University of Cologne, electronic publication of PhD Theses requires compound documents in PDF–format. On the other hand a simple file format, as for instance ascii-text files, are desirable to have an easy access to datasets. In this appendix, data are formatted as simle text documents and then transformed into PDF format. Thus, one can easily use the “Select Text” option in a PDF viewer (adobe acrobat reader) to copy datasets and paste them into text files. Tables are saved row by row with fields separated by semicolons. Ends of rows are marked by the insertion of “XXX”. Note: In order to recreate a comma separated file (for import into Microsoft Excel) from the texts saved here: 1) all line breaks have to be removed and then 2) the triple XXX has to be replaced by a line break (can be done in a text editor). Otherwise, datasets are composed as indicated in the individual descriptions. Chapter 1 - Supplementary Table 1: Sampled Populations, localities with coordinate data, river basins and references to other studies. Drainage No. Locality GIS References System Volckaert et al. 50°47′N 4°30′ 1 River Neet, S. England River Neet 2002, W Hänfling et al. 2002 52°51'N 2 River Dove, North Yorkshire River Trent 1°37'W Volckaert et al. 2002, 50°48'N River 3 Stream Molebeek-Maarkebeek, Belgium Hänfling et 3°41'E Scheldt al. 2002, Knapen et al. 2003 Volckaert et al. 50°24′N River 4 Stream Trouille, Belgium 2002; 4°00′E Scheldt Hänfling et al. 2002 Volckaert et al. 50°15′N River 5 Stream St-Georges, France 2002; 3°31′E Scheldt Hänfling et al. 2002 52°42'N IJssel Sea, 6 Enkhuizerzand, Netherlands 05°24'E River Rhine 52°47'N IJssel Sea, 7 Wagenpad-West, Netherlands 5°11'E River Rhine Mouth of the River Sieg into the 8 Rhine, North Rhine-Westphalia, 50°46'N 7°6'E River Rhine Germany Lower Sieg at Autobahn BAB 59 west 9 of Siegburg, North Rhine- 50°48'N 7°9'E River Rhine Westphalia, Germany Lower Sieg at Siegburg, North Englbrecht 10 50°48'N7°11'E River Rhine Rhine-Westphalia, Germany et al. 2000 Lower Sieg at Buisdorf, North 50°47'N 11 River Rhine Rhine-Westphalia,Germany 7°13'E River Sieg at Lauthausen, North 50°47'N 12 River Rhine Rhine-Westphalia,Germany 7°19'E Stream Pleis, outlet into the River 50°46'N River Sieg, Englbrecht 13 Sieg at Niederpleis, North Rhine- 7°12'E River Rhine et al. 2000 Westphalia, Germany Lowest reaches of Düssel at 51°17'N Englbrecht 14 Kaiserswerth, North Rhine- River Rhine 6°43'E et al. 2000 Westphalia,Germany River Mosel at Koblenz, Rhineland- 50°23'N 15 River Rhine Palatinate, Germany 7°35'E River Mosel at St. Aldegund, 16 50°5'N 7°8'E River Rhine Rhineland-Palatinate, Germany Stream Rur at Düren, tributary to 50°46'N River Maas, 17 the River Maas, North Rhine- 6°29'E River Rhine Westphalia, Germany River Stream Dhünn in Leverkusen, North 50°49'N 18 Wupper, Rhine-Westphalia,Germany 6°28'E River Rhine Stream Glasewasser southeast of 41°40'N River Lippe, 19 Paderborn, North Rhine-Westphalia, 8°58'E River Rhine Germany Stream Morsbach east of Solingen at River 20 Bridge of B229 across River Wupper, 51°10'N 7°8'E Wupper, North Rhine-Westphalia, Germany River Rhine River Wupper below Stausee River 51°16'N 21 Beyenburg, North Rhine-Westphalia, Wupper, 7°16'E Germany River Rhine Stream Schlingenbach above its 50°59'N River Sieg, 22 Mouth into the River Agger, North /°20'E River Rhine Rhine-Westphalia, Germany Stream Bröl north of Schönenberg, 50°51'N River Sieg, 23 North Rhine-Westphalia, Germany 7°26'E River Rhine Stream Bröl between Bröl and 50°50'N River Sieg, 24 Winterscheidt, North Rhine- 7°22'E River Rhine Westphalia, Germany Stream Logebach east of Ittenbach, River Sieg, 25 50°42N 7°17'E North Rhine-Westphalia, Germany River Rhine Stream Naaf east of Wahlscheid, 50°53'N River Sieg, 26 North Rhine-Westphalia, Germany 7°15'E River Rhine Upper Sieg at Netphen, North Rhine- River Sieg, 27 50°55'N 8°6'E Westphalia, Germany River Rhine Stream Ottersbach at Eitorf, North 50°47'N River Sieg, Englbrecht 28 Rhine-Westphalia, Germany 7°26'E River Rhine et al. 2000 Stream Mengbach in the west of 50°47'N River Sieg, 29 Eitorf, North Rhine-Westphalia, 7°25'E River Rhine Germany Stream Fokkenbach east of 50°33'N River Wied, 30 Niederbreitbach, Rhineland- 7°77'E River Rhine Palatinate, Germany Stream Flaumbach south of Treis- River 50°11'N 31 Karden, Hunnsrück, Rhineland- Mosel, River 7°17'E Palatinate, Germany Rhine Stream Kyll south of Gerolstein, River 50°14'N 32 Eifel, Rhineland-Palatinate, Mosel, River 6°39'E Germany Rhine River Mosel at Kenn, Rhineland- 49°49'N 33 River Rhine Palatinate, Germany 6°42'E Stream Ruwer at Mandern south of River 49°37'N Englbrecht 34 Trier, Hunnsrück, Rhineland- Mosel, River 6°36'E et al. 2000 Palatinate, Germany Rhine River Stream Elzbach at Mannebach, Eifel, 50°17'N 35 Mosel, River Rhineland-Palatinate, Germany 6°59'E Rhine Stream Rotes Wasser north of Bracht 50°56'N River Lahn, 36 and Marburg, Hesse, Germany 8°51'E River Rhine Stream Wisper east of Lorch, 37 50°3'N 7°51'E River Rhine Taunus, Hesse, Germany Ailsbach at Ahorntal, Upper 49°49'N River Main, Englbrecht 38 Franconia, Germany 11°24'E River Rhine et al. 2000 Stream Mucklbrunnenbach at 50°22'N River Main, Englbrecht 39 Tremersdorf, Upper Franconia, 10°57'E River Rhine et al. 2000 Germany Stream Schorgast, tributary to 50°07'N River Main, Englbrecht 40 River Weisser Main, Upper 11°33'E River Rhine et al. 2000 Franconia, Germany Stream Salzbach at Hinterweidental, River 49°12'N 41 Pfälzer Wald, Rhineland-Palatinate, Lauter, 7°44'E Germany Upper Rhine Stream Sauerbach at Hirschtal, River Sauer, 42 Pfälzer Wald, Rhineland-Palatinate, 49°4'N 7°45'E Upper Rhine Germany Stream Hochspeyerbach at 43 Frankeneck, Pfälzer Wald, 49°23'N 8°3'E Upper Rhine Rhineland-Palatinate, Germany Stream Wellbach west of Rinntal, River 49°14'N 44 Pfälzer Wald, Rhineland-Palatinate, Queich, 7°55'E Germany Upper Rhine Stream Maisach at Lindenberg, 50°3'N Englbrecht 45 Upper Rhine Bavarian Swabia / Bregenzer Ach 11°20'E et al. 2000 Stream Kapeller Neubach at 47°39'N Englbrecht 46 Upper Rhine Gestraz, Bavarian Swabia / Leiblach 9°59'E et al. 2000 Reuss at Rotkreuz, Aargau, Alpine 47 47°8'N 8°24'E Switzerland Rhine Goldbach at Steinerberg, Aargau, 48 47°2'N 8°34'E Alpine Rhine Switzerland River Alz at Truchtlaching, Upper 47°58'N River Inn, 49 Bavaria, Germany 12°30'E Upper Danube Stream Rimbach at Hofgiebing, Upper 48°14'N River Inn, 50 Bavaria, Germany 12°14'E Upper Danube Stream Grosse Ohe at Spiegelau, 48°55'N Englbrecht 51 Upper Danube Bavaria, Germany 13°22'E et al. 2000 Lake Königsee at St. Bartholomä, 47°34'N River Inn, Englbrecht 52 Bavaria, Germany 12°59'E Upper Danube et al. 2000 River Teisnach at Patersdorf, 49°1'N River Regen, Englbrecht 53 Bavaria, Germany 12°59'E Upper Danube et al. 2000 Stream Wielenbach at Kronholz, 48°31'N River Lech, Englbrecht 54 Upper Bavaria, Germany 13°11'E Upper Danube et al. 2000 Stream Visbecker Aue northwest of 52°55'N River Hunte, Englbrecht 55 Wildeshausen, Lower Saxony, Germany 8°25'E River Weser et al. 2000 River Werra at Hedemünden, Lower 51°24'N 56 River Weser Saxony, Germany 9°46'E Stream Forstbach between Negenvorn 51°54'N 57 River Weser and Golmbach, Lower Saxony, Germany 9°33'E Stream Maibolte east of Lemgo, 58 52°3'N 8°57'E River Weser North Rhine-Westphalia, Germany Stream Lünzener Bruchbach east of 53°08′N River Wümme, 59 Scheeßel, Lower Saxony, Germany 9°32′E River Weser Stream Gehlsbach at Klein Pankow, 53°22'N River Elde, 60 Mecklenburg-Western Pomerania, 12°1'E River Elbe Germany Stream Seeve below Wehlen, Lower 53°17'N 61 River Elbe Saxony, Germany 9°54'E Stream Krabach at Bach, North 50°45'N River Sieg, Englbrecht 62 Rhine-Westphalia, Germany 7°24'E River Rhine et al. 2000 Stream Giertshagener Bach at 50°45'N River Sieg, Englbrecht 63 Giertshagen, North Rhine- 7°36'E River Rhine et al. 2000 Westphalia, Germany Stream Wahnbach, Outlet into River 50°48'N River Sieg, Englbrecht 64 Sieg at Seligenthal, North Rhine- 7°16'E River Rhine et al. 2000 Westphalia, Germany Volckaert River Nete, et al. 53°14'N 65 Witte Nete, Flanders, Belgium River 2002, 5°04'E Scheldt Knapen et al. 2003 Volckaert et al. 53°14'N River 66 Zwaanebeek, Flanders, Belgium 2002, 4°34'E Scheldt Knapen et al. 2003 Chapter 1 - Supplementary Table 2: SNP Loci, primers and variable sites with ancestral and derived states in phylogeographic lineages of the Cottus gobio complex.