Leader in Biomolecular Solutions for Life Science ALOX15B Rabbit pAb Catalog No.: A6865 Basic Information Background Catalog No. This gene encodes a member of the lipoxygenase family of structurally related nonheme A6865 iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, Observed MW while metabolizing linoleic acid less effectively. This gene is located in a cluster of 76kDa related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different Calculated MW isoforms have been described. 67kDa/69kDa/72kDa/75kDa Category Primary antibody Applications WB, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 247 O15296 IF 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALOX15B (NP_001132.2). Synonyms ALOX15B;15-LOX-2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using ALOX15B antibody (A6865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. Immunofluorescence analysis of NIH/3T3 Immunofluorescence analysis of PC-12 Immunofluorescence analysis of U2OS cells using ALOX15B Rabbit pAb (A6865) at cells using ALOX15B Rabbit pAb (A6865) at cells using ALOX15B Rabbit pAb (A6865) at dilution of 1:100 (40x lens). Blue: DAPI for dilution of 1:100 (40x lens). Blue: DAPI for dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. nuclear staining. nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..