MS. MANITA ARYAL PERSONAL DETAILS Address: House No-3, Swagat Margh, Lokhanthali, Madhyapur (Thimi) -16, Bhaktapur, Nepal. Contact Number: +977 1 6631077(R), +977 9841810653(M) or +977 9851140699 (M) Email:[email protected] GPO Box:-5685 Kath Data of Birth: 17 February, 1983 Nationality: Nepali Passport Number: 05717226 MEMBERSHIP Life time member of NESOM (Nepalese Society of Microbiology) Young Scientist Membership of ESCMID (European Society of Clinical Microbiology and Infectious Diseases) EDUCATION Master of Science, Specialization in Medical Microbiology 2007 Tribhuvan University, Kathmandu, Nepal with Distinction Thesis Title: Isolation, identification and antibiotic sensitivity testing of Salmonella serovars from enteric fever suspected patients visiting Bir hospital. Bachelor of Science, Microbiology 2005 Tribhuvan University, Kathmandu, Nepal with First Division Project Title: Microbial analysis of paper money from different places of Kathmandu valley TRANINGS and SEMINARS ESCMID Postgraduate Education Course “Hospital-Acquired Infections: Understanding the Magnitude and Needed Interventions”, Muscat Oman, 24-25 March, 2017 ESCMID Postgraduate Technical Workshop “Anaerobic Bacteria: Next Generation Technology Meets Anaerobic Diagnostics” specializing on bioinformatics, University Medical Center Groningen, The Netherlands, September 26 - 28, 2016. Second National Summit of Health and Population Scientists in Nepal, April 11 -12, 2016. Workshop on Data Analysis using SPSS. Organized by Central Department of Microbiology (CDM), Tribhuvan University (TU), Nepal, June 25-27, 2015. Workshop in Research methodology, Organized by CDM, TU, Nepal, July 27-28, 2014. Workshop on Scientific Writing and Publishing, Jointly organized by American Society for Microbiology (ASM) and CDM, TU on June 23, 2013 1 Aspergilosis (AAA) conferences, Istanbul Turkey, January 26 – 28, 2012 Writing Scientific Paper organized jointly by Shi-Ghan Health Foundation in Collaboration with Nat’I Inst of Trop Med and Public Health Research, January 26 -28, 2009. Training workshop on Bioinformatics organized jointly by Microbiological Students Association of Nepal (MiSAN) and CDM,TU, June 12-14, 2007 Molecular Biology Training at RLABB (Research Laboratory for Biotechnology and Biochemistry), May 21- June 6, 2007 RESEARCH INTEREST Infection control and infectious diseases (E. coli, Salmonella Typhi, Vibrio cholerae, Mycobacterium tuberculosis) Anaerobic Microbiology Water quality analysis Waste water Technology RESEARCH CONDUCTED Detection of V. cholerae from Surface Water of Kathmandu Valley. Research grant provided by Research Center, Institute of Science and Technology, Tribhuvan University, Kathmandu, Nepal. Detection of Salmonella species from Surface Water of Kathmandu Valley. Research grant provided by University Grants Commission, Sanothimi, Bhaktapur, Nepal Study of air mycoflora of Kathmandu Valley Drinking water quality of Nepal. RELEVANT WORK EXPERIENCE Central Department of Microbiology, Kirtipur, Nepal Lecturer and Researcher 2011 December to till date Lecturing in Master level classes on: General Microbiology (Microbial Systematics, Structure and Physiology), Microbial Genetics and Molecular Biology, Microbial Systematics, Microbial Biotechnology and Pharmaceutical Microbiology, Public Health Laboratory, Systematic and Diagnostic Virology, Systematic and Diagnostic Mycology. Conducting Laboratory Classes for Master level classes on: Microbial Genetics and Molecular Biology, Biochemistry and Instrumentation, Human Anatomy and Physiology, Microbial Biotechnology and Pharmaceutical Microbiology, Public Health Laboratory, Systematic and Diagnostic Virology. Supervising students during their dissertation. Patan Academy of Health Sciences, Lalitpur, Nepal Lecturer June 2011 to November 2011 2 Lecturing for MBBS students on General and Medical Microbiology. Amrit Campus, Lainchour Nepal Lecturer and Research Assistant April 2009 to May 2011 Lecturing in Undergraduate level classes on:- Introductory Bacteriology, Growth and physiology of Bacteria, Fundamental Biochemistry of Microbiology, Microbial Genetics, Bio Molecules, Microbial Metabolism, Instrumentation, Microbial Biotechnology (Agriculture, Fermentation, Dairy, Enzyme), Bioinformatics, Medical Microbiology (Medically important Viruses, Bacteria, Fungi), Methods of identifying and typing bacteria, Infection and immunity, Pharmaceutical Microbiology Conducting Laboratory classes for undergraduate level on 3 different levels of Microbiology (General Microbiology, Biotechnology, Biochemistry and Medical Microbiology) Project supervising TECHNIQUES KNOWN Nucleus staining (Prokaryotic and Eukaryotic cells), Nucleic acid Extraction- Extraction and purification of chromosomal and plasmid DNA from bacteria, Extraction and purification of DNA from yeast cells, Extraction and purification of RNA from Prokaryotic and Eukaryotic cells, Confirmation of DNA extraction by Agarose Gel Electrophoresis, DNA Transformation experiment, Detection and isolation of mutants, Plasmid Profiling, RAPD-PCR, SDS Page, Western Blotting Microbiological techniques: Microscopy and staining Techniques, Isolation, Enumeration, Identification and Biochemical characterization of microbes, Quality control on study of different media and reagents. Instruments well versed with: Centrifuges, Colorimeters, UV-Visible Spectrophotometer, Colony Counter, Autoclave, Microscopes Immunological Techniques: Agglutination, Precipitation, ELISA COMPUTER SKILLS Quantitative data analysis (descriptive analysis, inferential analysis- chi square, t-test, ANOVA, co-relation, 95% CI, Standard error, Odds ratio, p-value, Regression analysis). Data entry and Data analysis using computer software (SPSS, STATA, SAS, Epi Info). Bioinformatics PUBLICATION 3 Aryal, M. (2015). Detection of Salmonella species from surface water of Kathmandu Valley. European Journal of Biomedical and Pharmaceutical Sciences, 596-600. Aryal, M., Sharma, S., Jyotshana, S., & Bhatta, D. R. (2011). Seroprevalence of enteric fever by Widal test. Tribhuvan University Journal of Microbiology, 78-81. Sharma, S., Pokhrel, B. M., Manita, A., & Bhatta, D. R. (2011). Antibiotic susceptibility pattern of Gram negative urinary isolates from post operative cardiac patients. Tribhuvan University Journal of Microbiology, 73-77. Subedi, M., & Aryal, M. (2010). Public perception about drinking jar water and its bacteriological analysis. Nepal Medical College Journal, 110-114. POSTER/ ABSTRACT/ PAPER PUBLICATION AND PRESENTATION Poster presented in Second National Summit of Health and Population Scientists in Nepal April 11-12, 2016 "Isolation of Salmonella species from Bagmati River" Poster presented in Aspergilosis (AAA) conferences 2012, Istanbul Turkey "Study of allergens produced by Aspergillus flavus and A. fumigates isolated from the atmosphere of Kathmandu Valley, Nepal." Poster presentation in Aspergilosis (AAA) conferences 2012, Istanbul Turkey “In vivo allergic immune responses of Aspergillus species isolated from Kathmandu Valley, Nepal” Abstract publication on International Symposium on Biotechnology, 2009. "Microbial Analysis of paper money from different places of Kathmandu valley." Abstract publication on 6th Congress of Association of clinical pathologist of Nepal, 2008 "Comparative study on diagnosis of typhoid fever in Bir Hospital Nepal " Publication of article in the Himalayan times on 19th January 2009 "Taking about Mineral Water " MASTERS THESIS SUPERVISED Antibiogram and plasmid profiling of Beta lactamases producing E. coli isolated from UTI suspected pediatric patients. Comparative profile of biofilm producing and non-producing Acinetobacter calcoaceticus– Acinetobacter baumannii complex from different clinical specimens. Comparision of nitrate reductase assay and gene xpert mtb/ rif for detection of rifampicin resistant Mycobacterium tuberculosis in Nepal Tuberculosis Center. Study of cellulolytic fungi isolated from soil and compost. Drug resistance pattern of bacterial pathogens of Enterobacteriaceae family. 4 Detection of AmpC beta lactamase in Enterobacteriaceae isolates among patients visiting Shahid Gangalal National Heart Centre. Detection of SHV, TEM and CTX-M type extended spectrum β-lactamase in E. coli. 5 .