Catalog # Aliquot Size H811-40N-10 10 µg H811-40N-100 100 µg H811-40N-1000 1 mg Recombinant Human HCC-1 (CCL14) Recombinant human protein expressed in E. coli cells Catalog # H811-40N Lot # G3211-49 Product Description Specific Activity Recombinant Human HCC-1 (CCL14) was expressed in E. coli cells. The protein accession number is Q16627 Gene Aliases C-C motif chemokine 14, Chemokine CC-1, HCC1, HCC-3, HCC- 1(1-74), NCC-2, Small-inducible cytokine A14, CCL14, NCC2, SCYA14 Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method. Formulation Determined by its ability to chemoattract human monocytes using a concentration range of 2-40 ng/mL. Recombinant HCC-1 was lyophilized from a 0.2 µm filtered 20 mM PB,100 mM NaCl solution pH 7.5. Purity Reconstitution Protocol Recombinant Human HCC-1 (CCL14) resolved on a 17% SDS- A quick spin of the vial followed by reconstitution in distilled water PAGE gel under reducing to a concentration not less than 0.1 mg/mL. This solution can then conditions and stained with be diluted into other buffers. Coomassie Brilliant Blue G-250 Storage and Stability Approx. MW 9.0 kDa The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six Recombinant Human HCC-1 months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. (CCL14) Recombinant human protein expressed in E. coli cells Scientific Background Catalog Number H811-40N Hemofiltrate C-C chemokine (HCC)-1 is a recently described monocyte Specific Lot Number G3211-49 chemoattractant. CCL14 (also known as HCC-1) belongs to the CC Purity 95% chemokine family. Its mature propeptide is a low-affinity agonist of CCR1 Stability 6 mos. at –20oC from date of shipment that is converted to a high-affinity agonist of CCR1 and CCR5 on proteolytic Storage & Shipping The lyophilized protein is stable for at processing by serine proteases. Determination of the amino acid sequence least one year from date of receipt at - of HCC-1 revealed four cysteine residues in positions characteristic of the 70°C. Upon reconstitution, this cytokine C-C chemokine family, and comparison with the sequences of other can be stored in working aliquots at 2° chemokines revealed that HCC-1 was most homologous to MIP-1a. - 8°C for one month, or at -20°C for six However, several functional properties of HCC-1 were atypical of months, with a carrier protein without chemokines. Unlike other chemokines, HCC-1 was expressed constitutively detectable loss of activity. Avoid in a number of tissues and was present at high concentrations in normal repeated freeze/thaw cycles. human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .