See discussions, stats, and author profiles for this publication at: http://www.researchgate.net/publication/253646313 Species of Botryosphaeriaceae involved in grapevine dieback in China ARTICLE in FUNGAL DIVERSITY · JULY 2013 Impact Factor: 6.22 CITATIONS READS 5 103 14 AUTHORS, INCLUDING: Wei Zhang Yong Wang Fudan University Guizhou University 1,001 PUBLICATIONS 9,595 CITATIONS 41 PUBLICATIONS 237 CITATIONS SEE PROFILE SEE PROFILE Jian-Kui Liu Youliang Peng Guizhou Academy of Agricultural Sciences China Agricultural University 39 PUBLICATIONS 260 CITATIONS 83 PUBLICATIONS 1,648 CITATIONS SEE PROFILE SEE PROFILE Available from: Jian-Kui Liu Retrieved on: 06 October 2015 Fungal Diversity (2013) 61:221–236 DOI 10.1007/s13225-013-0251-8 Species of Botryosphaeriaceae involved in grapevine dieback in China Ji-Ye Yan & Yue Xie & Wei Zhang & Yong Wang & Jian-Kui Liu & Kevin D. Hyde & Robert C. Seem & Guo-Zhen Zhang & Zhong-Yue Wang & Sheng-Wei Yao & Xian-Jin Bai & Asha J. Dissanayake & You-Liang Peng & Xing-Hong Li Received: 9 May 2013 /Accepted: 25 June 2013 /Published online: 19 July 2013 # Mushroom Research Foundation 2013 Abstract Botryosphaeria dieback is a serious disease prob- study also revealed considerable differences in the geograph- lem for table and grape wine production worldwide. The ical distribution of Botryosphaeriaceae species in China disease however, has been less well-studied in China. In this with Lasiodiplodia theobromae and Neofusicoccum parvum study, we surveyed Botryosphaeria dieback in 72 vineyards occurring only in subtropical monsoon climate regions, of 20 grape-growing regions in China and found that Diplodia seriata occurring only in temperate monsoon cli- Botryosphaeria dieback occurs in 18 out of 20 provinces. mate regions, and Botryosphaeria dothidea occurring in both Morphological and multi-gene phylogenetic analyses con- temperate and subtropical monsoon climate regions. Analysis firmed that Botryosphaeria dothidea, Diplodia seriata, of 26 isolates showed that there is little genetic variation Lasiodiplodia theobromae and Neofusicoccum parvum are within species. Koch’s postulates were satisfied for all species, associated with different grapevine dieback symptoms. This and pathogenicity testing showed that among the 25 major cultivars growing in China, none was resistant to the four taxa. The current paper represents the first detailed report on : : : : J.<Y. Yan W. Zhang S.<W. Yao A. J. Dissanayake Botryosphaeria dieback of grapevines in China. X.<H. Li (*) Institute of Plant and Environment Protection, Keywords Botryosphaeria dieback . Botryosphaeriaceae . Beijing Academy of Agriculture and Forestry Sciences, . Beijing 100097, People’s Republic of China Grapevine Pathogenicity e-mail: [email protected] < : : < : < * J. Y. Yan Y. Xie G. Z. Zhang Y. L. Peng ( ) Introduction Department of Plant Pathology, China Agricultural University, Beijing 100193, People’s Republic of China e-mail: [email protected] Grapevine trunk diseases are some of the main factors limiting both vineyard longevity and productivity (Pascoe 1998;van Y. Wang Niekerk et al. 2006;Martosetal.2008; Úrbez-Torres 2011; Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, People’s Republic of China Qiu et al. 2011). Woody parts of the vine are infected by the : fungi, which lead to serious decline of the vineyard (Gubler J.<K. Liu K. D. Hyde et al. 2005). It has been reported that the annual value of grape Institute of Excellence in Fungal Research, School of Science, production in California is approximately US$ 23 billion, but Mae Fah Luang University, Chiang Rai 57100, Thailand losses caused by trunk diseases were more than US$ 260 R. C. Seem million (Siebert 2001). Grapevine trunk diseases are primarily Department of Plant Pathology, New York State Agricultural caused by ascomycetes, plus a few basidiomycete species. Experiment Station, Cornell University, Geneva, NY 14456, USA The fungi normally infect vines through wounds and natural Z.<Y. Wang openings, causing decline of the vine as a result of vascular Institute of Plant Protection, Chinese Academy of Agricultural colonization and/or toxin production. Grapevine trunk dis- Sciences, Beijing 100193, People’s Republic of China eases currently include black foot, Botrysophaeria dieback, Esca, Eutypa dieback, Phomopsis dieback and Petri disease X.<J. Bai Guangxi Fruit and Vegetable Research Institute, Guilin 541004, (Pascoe 1998; Úrbez-Torres et al. 2006; Úrbez-Torres and People’s Republic of China Gubler 2011; Hofstetter et al. 2012). Botryosphaeria dieback 222 Fungal Diversity (2013) 61:221–236 is an import cause of trunk diseases. It has been suggested that Fig. 1 a Provinces in China surveyed for grape Botryosphaeria dieback. grapevine Botryosphaeria dieback is the most appropriate name b Distribution of Botryosphaeriaceae species causing grapevine Botryosphaeria dieback in China. XJ: Xinjiang Province, NX: Ningxia for the disease caused by Botryosphaeriaceae and is thus used Hui Autonomous Region, HLJ: Heilongjiang Province, LN: Liaoning in this paper (Úrbez-Torres et al. 2006; Úrbez-Torres 2011). Province, BJ: Beijing City, HEB: Hebei Province, SD: Shandong Prov- Botryosphaeria dieback, caused by species in the ince, SX: Shanxi Province, SHX: Shan’Xi Province, GS: Gansu Prov- Botryosphaeriaceae, has been reported in almost all main ince, HEN: Henan Province, JS: Jiangsu Province, AH: Anhui Province, HUB: Hubei Province, SC: Sichuan Province, CQ: Chongqing City, SH: grape-growing countries, including Australia, Chile, China, Shanghai Province, ZJ: Zhejiang Province, HUN: Hunan Province, GX: Egypt, France, Hungary, Lebanon, Portugal, South Africa, Guangxi Province Spain, Italy and the USA. Botryosphaeria dieback has been reported to cause serious losses as a result of shoot dieback, represent a potential threat to the emergent grapevine indus- accompanied by elongated oval-shaped cankers in the trunk try. However, no detailed information on the occurrence of and often results in shriveling, rotting and dropping of clusters Botryosphaeria dieback in China is available. Consequently, in some countries (El-Goorani and El-Meleigi 1972; Lehoczky the aims of this study are as follows: (1) to evaluate the 1974; Leavitt and Munnecke 1987; Rovesti and Montermini importance of Botryosphaeria dieback in China by means 1987; Larignon and Dubos 2001; Castillo-Pando et al. 2001; of vineyard surveys; (2) to identify Botryosphaeriaceous Phillips 2002; Auger et al. 2004; van Niekerk et al. 2004; taxa associated with grapevine trunk disease by morpholog- Luque et al. 2005; Choueiri et al. 2006;Lietal.2010; Úrbez- ical and molecular approaches; (3) to clarify the geographical Torres 2011). Members of the Botryosphaeriaceae are well- distribution of the taxa associated with grapevine dieback in known pathogens causing cankers and dieback in many woody China; and (4) to determine the pathogenicity of the different plants, and some of them are considered endophytes, weak Botryosphaeriaceae species in the main grapevine cultivars pathogens or are associated with cankers in both introduced growing in China. and native tree hosts (Maas and Uecker 1984; Rumbos 1987; Millholland 1991; Phillips 2002; van Niekerk et al. 2004; Slippers and Wingfield 2007; Pérez et al. 2010; Amponsah Materials and methods et al. 2011). Botryosphaeria dieback has been reported to be caused by 20 different botryosphaeriaceous taxa worldwide, Vineyard survey and fungal isolation including Botryosphaeria dothidea (Moug.) Ces. & De Not., Diplodia corticola A.J.L. Phillips et al., D. mutila (Fr.) Mont., At least two different vineyards were surveyed in each prov- D. sarmentorum (Fr.) Fr., D. seriata De Not., Dothiorella ince throughout all main grapevine-growing provinces in americana Úrbez-Torres et al., Do. iberica A.J.L. Phillips China between 2009 and 2012 to determine the occurrence et al., Lasiodiplodia crassispora T.I. Burgess & Barber, L. of Botryosphaeria dieback (Fig. 1a). Characteristic symptoms, theobromae (Pat.) Griffon & Maubl., L. viticola Úrbez-Torres such as shoot death, elongated oval-shaped cankers in trunks, et al., L. missouriana Úrbez-Torres et al., Neofusicoccum aus- shriveled, dropped and rotted fruit clusters, and whole plant trale (Slippers et al.) Crous et al., N. luteum (Pennycook & death were recorded and photographed in vineyards. Samples Samuels) Crous et al., N. macroclavatum (T.I. Burgess et al.) were collected and taken to the laboratory for pathogen isola- T.I. Burgess et al., N. parvum (Pennycook & Samuels) Crous tion. Diseased tissue samples from symptomatic vines, fruits et al., N. mediterraneum Crous et al., N. ribis (Slippers et al.) or vine nodes were also photographed, recorded and then Crous et al., N. viticlavatum (Van Niekerk & Crous) Crous stored in an incubator (at 4 °C) for later studies. Isolations et al., N. vitifusiforme (Van Niekerk & Crous) Crous et al. and were made from small pieces of canker tissue cut from the Phaeobotryosphaeria porosa (Van Niekerk & Crous) Crous & margin between necrotic and healthy tissues. Samples were A.J.L. Phillips (Ma et al. 2001;Siebert2001; Taylor et al. 2005; surface-sterilized in 75 % ethanol for 1 min and then rinsed Savocchia et al. 2007; Úrbez-Torres et al. 2007a, b, 2010a, b, three times in sterilized water before culturing on potato 2012; Úrbez-Torres and Gubler 2009; Billones et al. 2010). dextrose agar (PDA) at 28 °C. Single conidia were transferred However, the incidence, virulence and symptoms